Scanning Electron Microscopy (SEM) Lecture with Animations and Real Measurement

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Scanning Electron Microscopy (SEM) is explained in this video and using a scanning electron microscope a sample is studied for demonstration. The first part of the lecture is dedicated for learning the basics with the help of animations and the second part shows how the real SEM actually works.
The SEM demo measurement was done at the Institute of Physics, University of Tartu
Here is our short video series, where we use SEM to study a variety of samples from everyday life and popular science:
• Under the Scanning Ele...
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/ captaincorrosion
Visit our SEM image gallery:
captaincorrosion.com/gallery/

Опубликовано:

31 июл 2024

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Комментарии : 73

@amosakande6141 2 года назад

Best video on SEM ever! Thanks

@user-dq3pb5oj6v 4 года назад

Thank you for the demonstrating. It is really a clear and easy to understand the scanning electron beam by your method. Really Thanks.

@CaptainCorrosion 4 года назад

Hey! Thanks for the feedback. We really appreciate it!

@drjahanzeb1135 9 лет назад

Thanks Dr Maido. Sure today I will see you SEM images video.

@paulblasingame 6 лет назад

Great video. Thank you!

@sheetalmore2052 6 лет назад

Finally I got the basic concept atlest .😫. Thanks so much.. 😘

@youngs1072 7 лет назад

It is a great and very useful video about SEM. Thank you, my friend from Estonia.

@CaptainCorrosion 7 лет назад

Thank you for watching our video!

@muratoztrgt7625 3 года назад

Nice video. I saw and learned many things. Thank you.

@drjahanzeb1135 9 лет назад

Thanks Maido.... it is very informative.

@bludog4657 7 лет назад

Very nice. Thank you for the details in every step of the process.

@CaptainCorrosion 7 лет назад

Thanks a lot! We might make an updated version of the video at some point in the future as the hardware of our company received a major upgrade, allowing us to make better animations.

@MimiTai677 Год назад

Nice video presentation and exaplanation

@arcanity4343 9 лет назад

Hi Maido, This is probably the most helpful video about the SEM I've watched, you're using a modern SEM and you're not making it too complicated, thanks a lot! The only thing that I wish you had shown was the elemental composition analysis because that still confuses me a bit whenever I try it. One question: how do you decide which accelerating voltage to use for studying a certain specimen, and why is it important?

@arcanity4343 9 лет назад

***** Thank you, that's very helpful! Yeah, the part when the analysis program gives me weird elements for the peaks is what I'm having problems with, but I hope that as I use the SEM more it'll be much easier. Glad to know that I'm not the only one for whom that happens to. Thanks :)

@imebasseyisua-ikoh4167 8 лет назад

+Maido This is just what i needed. Just got better understanding of SEM-EDX. Thank you

@ronaksinghbansal7269 6 лет назад

You have a very Excellent way of teaching and your university have a very good lab.

@CaptainCorrosion 6 лет назад

Thanks!

@amudaabdulgafarkayode9185 5 лет назад

Great video. Quite illustrative!

@CaptainCorrosion 5 лет назад

Thank you Sir!

@filmitech8605 2 года назад

Very informative lecture Sir.

@jaykumarvaidya1662 7 лет назад

Thanks

@jyotid8866 6 лет назад

Very nicely explained Thank you so much

@CaptainCorrosion 6 лет назад

Thanks! It was one of our first videos and perhaps we might make a more detailed and better one in the future. Learned a few more tricks since then =)

@robertonoeloyanin5262 8 лет назад

This is the Great Thing To US!!! Thank you Scientists!! :) :-)

@CaptainCorrosion 8 лет назад

Captain Corrosion at your service!

@youcefdjedi5563 9 лет назад

hello Maido you are required to analyze a biological sample by SEM. How can you eliminate the differences between the properties of the sample and the physical properties of SEM? thank you. الترجمة

@rabeaeshkal1889 7 лет назад

really thanks for this video

@CaptainCorrosion 7 лет назад

You are welcome! Might make an updated and more detailed version in the future though as Ive got more experience now with this technique.

@mehrnooshalemi7894 5 лет назад

It was the best video which I found for SEM. Would you please also make a video which describes how to prepare a good sample for the test?

@CaptainCorrosion 5 лет назад

Thanks! Actually we have a whole series dedicated to sample preparation as each case requires a unique approach. Here is the link to the videos: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-fnRnoJigsu0.html

@manovas1731 5 лет назад

how to identify the different composition elements and level of distribution

@GaryHB57 9 лет назад

Arcanity, the choice of accelerating voltage, or beam energy, is complicated. First, in most SEMs, resolution is better at higher voltages. If you are lucky enough to have a modern field emission system, then you can go to very low voltages at high mags. Besides that, more surface detail is visible at low energies. Also, less damage happens at low energy. Finally, sample charging depends on beam energy. If you play with the accelerating voltage for nonconducting samples, then you might get lucky and find a value for which the primary electron flux balances the secondary and backscattered electron fluxes, and charging can be minimized. That is tricky business. In general, use the lowest energy you can get away with and still have the resolution that you need. Of course, if you do EDX, then you must use certain voltages to get the signal that you are looking for.

@vinaypatil8728 Год назад

Great

@Shumayal 5 лет назад

Excellent video. I loved it. Only thing missing was EDS.

@CaptainCorrosion 5 лет назад

Thanks for the feedback. You might want to check out our "under the scanning electron microscope" series, where we also use EDS :) ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-fnRnoJigsu0.html

@ravanansathesh 4 года назад

Hello, it is a nice video.. Am using zeiss EVO 18 floor model and Jeol 6000+ bench top both SEM...But ur Microscope is very nice and costly.. ❤️❤️

@CaptainCorrosion 4 года назад

Thanks! Please share your opinion on bench top SEM. We were thinking of getting one for the students to play with. The other ones here might be interested in your feedback as well since a bench top SEM is generally obtainable already at a low price.

@litzenjateandoenredbull9554 3 года назад

WOW!!!!

@TheMellowgirl 4 года назад

lovely video

@CaptainCorrosion 4 года назад

Thank you Mellowgirl!!!

@TheMellowgirl 4 года назад

you're welcome

@Moha85iq 5 лет назад

Hello In the case of the sample a dielectric material Is irradiated before imaging or not and why

@CaptainCorrosion 5 лет назад

Hello! Studying dielectric samples with SEM is difficult as every time you scan over it during imaging, it charges up - this means that electrons cannot easily escape the studied site, causing the sample to locally heat up and also the locally charged site interferes with the electron beam, causing additional artifacts on the image. So dielectric samples should be covered with a thin layer of metal (e.g. Pt by magnetron sputtering). Another alternative is to use ESEM, which doesn't require samples to be coated with metal.

@sahasrapriya8160 7 лет назад

A very useful video. Thank you very much. I have a question: doesn't the electron beam damage the sample ? and how thick can the sample be in order to withstand the electrons ? Thank you in advance !

@CaptainCorrosion 7 лет назад

Hello and thank you for the question! It really depends on the sample and the beam current. If the beam current is sufficiently high, then it is possible to drill a hole through any material. In scanning electron microscopy one should use as low beam current as possible when studying substrates that get easily damaged by electrons. Coating the sample with a thin layer of conductive material (Au, Pt) would also help a lot. I remember how I once had NaOH crystals on the substrate and they were destroyed rather fast under the electron beam (barely got 1 image). Conductive carbon nanotubes on the other hand are much easier to study as the charge and heat is quickly carried away. So ideally you can easily study nanoparticles if they are properly prepared (e.g. apply nanopowder on silver paste on a conductive holder).

@sahasrapriya8160 7 лет назад

Captain Corrosion thank you very much once again ! followed it better now.

@CaptainCorrosion 7 лет назад

You are welcome!

@nurulshahzira769 4 года назад

Hi. U have a great video here! I have a question. Can SEM analyze the microstructure of a metal surface that is covered with epoxy but with exposed area of 10mm^2 only??

@CaptainCorrosion 4 года назад

If the surface is covered with epoxy, then you'll see only the top layer with secondary electrons. However, with dielectric coatings like epoxy, you will need to apply a layer of conductive material on it (e.g. Pt, Au) via sputtering to be able to study the sample in SEM.

@nurulshahzira769 4 года назад

@@CaptainCorrosion understand sir. Thank you so much! Im studying corrosion on magnesium plate. Wish me luck ☺

@CaptainCorrosion 4 года назад

@@nurulshahzira769 Good luck!

@sheetalmore2052 6 лет назад

But how this emitting electrons gives the image? I mean electrons are going into the detector then image? Silly question may be.. 😭 help me.

@CaptainCorrosion 6 лет назад

Hello and thank you for the question! When you irradiate the sample with a narrow beam of electrons, then you basically know the X and Y coordinates and measure the intensity of the signal (amount of electrons) coming from that location. The intensity can be understood as a Z coordinate, which is essentially a shade of grey, ranging from black to white. Therefore, by scanning row by row across the surface with the beam of electrons, you will get a data that consists of three coordinates and with a computer software you can easily convert it into an image. Note that in this method higher Z value (brighter spot) doesn't really mean its higher but rather it shows that we have a higher yield of electrons from that specific site.

@yashwanthshouri3534 7 лет назад

how can we measure the coating thickness

@CaptainCorrosion 7 лет назад

Hello and thank you for the question. It really depends on the substrate and coating as there are lots of methods to choose from. For instance, making cross-sections with focused ion beam and studying them with SEM. Or you can use special tools to cut precise slices of the material and image it from the side with SEM. We will publish a video in the end of march where we study a tooth under the scanning electron microscope - for that purpose we cut it into half as well.

@whynot345 5 лет назад

Sir, can tell me the exact application and outcome of the SEM process??

@CaptainCorrosion 5 лет назад

Heya! SEM has many applications - we try to cover some of them in our video series, where we study a variety of different samples. The link to the playlist is in the description of this video.

@Jitender_Chaurasia 8 лет назад

do we need to etch metal samples before doing SEM

@rupankaur7459 8 лет назад

+Jitender Chaurasia Yes, the sample should be etched to generate a better picture.

@CaptainCorrosion 8 лет назад

+Jitender Chaurasia Depends on what you want to see. Generally you only need to remove the "dirt" from metal substrates if you want to see the natural surface. If it contains oil leftovers from manufacturing or fat from fingers (mixed with salts), then you need to clean it with deionized water and organic solvents such as acetone. If the substrate is generally clean from such pollution, then a rubber syringe is good enough to remove the loose particles. Generally I wouldnt recommend etching - unless you are studying corrosion or want to remove the natural oxide layer for EDX analysis.

@CaptainCorrosion 8 лет назад

+Rupan Kaur The picture might be more interesting indeed after the etching as the sample would be covered with valleys and craters but generally any pre-treatment and cleaning/refreshing of the sample should be as non-destructive as possible!

@imranmirchohan781 Год назад

Can we find out the depth of pitting corrosion by SEM?

@CaptainCorrosion Год назад

We did that by making a cross-section with FIB and then studying it with SEM. If the pitting goes very deep, then you might as well try mechanical cross-sectioning.

@Mrswamy32 9 лет назад

can you tell me where can I download this animation ?

@Mrswamy32 9 лет назад

***** Thank you... I am lecturer, I am teaching SEM for Undergrads. if possible to provide this animation it will be very helpful... I will provide due credits to you in my classes. Can you please share this animation ? thanks

@Mrswamy32 9 лет назад

***** Thank you, I will look into it... very kind of you...

@dillipmohanty8345 4 года назад

Can we see the real atomic structure of metal though electron microscope sir

@CaptainCorrosion 4 года назад

Yes. But you will need a transmission electron microscope for that purpose and prepare a very thin sample. In the current video we use a scanning electron microscope, which is cannot see atoms but is great for studying surfaces with a resolution up to 1 nm.

@dillipmohanty8345 4 года назад

@@CaptainCorrosion thank you sir