siRNA mechanism | short interfering RNA mediated gene silencing 

Suman, I have watched all the videos of rnai, you are a true genius Suman, God bless you always

I have been following you from last years of my clg life, I'm a Micro stud and wanted to tell you that a lot and lot of students of NBU are crazy about you - it's like if we want to know any topic in deep ,we simply log in to you ...just to let you know that a hell lot of clg studs watch you and we talk about you and we all have seen the journey of your channel.... Thank you so much for being with us from our first yr to last one ❤

All the 4 videos of RNAi are awesome, it cleared my doubts regarding RNAi, thank you Sir.

You are a competent teacher.. And know how to convey your information in easier ways.. Thank you

Sir,You made me fall in love with Life Science.

I'm a med student and you helped me a lot about understanding the siRNA. Many thanks.

PLEASE BECOME A PROFESSOR. I learn more from your channel then from my professors.

I am learning all my topics from one and only yours channel seriiously. First thing first that comes in mind when i have a problem in any topic is "shomus biology". Thank you so much. 😍 love from Pakistan! Stay blessed 🤞

khub bhalo...sir ...this is the first time i am relying on any video lecture ...and its really fruitful.

Crystal clear. Great job.

Thanks to you I am doing better in my QBM class at UCF.

Thanku so much for such a valuable lacture.😀🤗

Sir, thank u very much you saved my time and made very easy to learn these things and watched your lectures more to study my molecular pharmacology subject.

Best video I have seen on this topic... thanks a lot

THANK YOU SIR for teaching this difficult topic in such a simple way .

Brilliantly explain the topic...👏👏👏

4 years ago, when I began to look for jobs, I studied on your channel. Now I am looking to change my job, I studied on your channel again. Thank you!

Thanku so much sir for your nice explanation 🙏

Thank you sir.Your videos help me a lot😊

Highly admirable teaching approach Suman, Keep it up. Very easy to understand such a complex process particularly for a beginner, applauds!!!!!!!

Respect for you.

Hi Suman sir...I feel the extreme joy of learning while watching your videos....Can you please make a video on RNA i antagonist process .....how the plant viruses suppress the RNA i ....

Thank you very very much for your detail introduction of RNAi machanism! you are the best teacher.

really amazing very clear concept.

wow.....Great explanation.....tq somuch...... .

no wrds for ur works ......really impressive ☺☺

Dada u r just too awesome,pls keep helping us n god bless u

U r best teacher.... thnkuuu so much fr making this concept so easy😊😊

dude you rock this was such an immense help!!!!

Thank you so much, this is such a wonderful and clear lecture! You really explained everything perfectly well. Thanks for your effort!

Bigger thanks for you Gad bless you 🙏🙏🙏🙏

Thank u so much sir , this topic is to easy to understand and thank u again

Amazing! Thank you ☺️☺️☺️!!

auspicious video dada....

Once I had dumped this rna interference part coz it was so hard..but now I understood everything and its easy..thanku so much sir..u are really awesome.

Suman, thank you so much for your videos. They really help to understand the concepts thoroughly!

You made my day

You are outstandingggggg. Love from Pakistan👍subscribed.

Very nice presentation

very helpful and very nicely explained.... thank you

my overseas professor of all time

No words to express 🤗

you are just fabulous.

Great, practice a bit on process flow fluency avoiding corrections and hesitations you have mastery over the subject

Excellent sir tq sooo much 🙏🙏🙏🙏🙏🙏🙏🙏🙏

thank you very much. very good explanation.

In your RNAi video you mentioned Argonaut to be RNAse type 3 but here you said Argonaut is RNAse H type. So which one is correct?

Amazing explanation 👏🏻

thank you for your trainer videos. I want to shoot video about siRNA designing on a specific example

u r d best.. sir thank you so mch fr ds amazng descriptn abt Rnai

No doubt great teacher you are😊..but plz make it clear that..only siRNA need NPC complex for its transportation (from nucleus to cytoplasm) or miRNA and shRNA also need????

very nice teaching sir.....

sir, RNase III is endonuclease so how can it cleave from ends (sides)??

Super very understanding thanks

Sir...If Dicer has endonuclease activity then why is it chopping off the siRNA from the sides as 9:30? Please explain it Sir. Thank you.

Hey you are amazing in teaching and make concept so clear it help me a lot in my net preparation. Do you have ecology videos also If so please reply??

very clear

Very useful sir, thank you sir

Thank you very much, you are amazing. I have been following your tutorials for a long time. But in this video I think there is a mistake, you said RISC is constructed of slicer and Argonaut, this is a mistake because the slicer is Argonaut, but other parts are components like GW182.

thank you so much sir really helpful

Sir, which books are u referred ? Plz tell me sir....it will help for my research work

gr8 how to do it practically on any cell..please show practical video or give us link

sir, its Very knowledge full video, it makes my whole concept clear about siRNA but i wanna know about the various types of siRNA like survivin and many more. So can u pls help me sir

Your videos are really a great help. Wanted to ask: Can we Use RNAi in immunising the cell against the RNA/ genetic information of HIV viruses?

Many thanks for the video. Very informative as usual. However, if I'm not wrong, there is a mistake. In the RNAi introductory video, you say Dicer is RNase H, but you say Argonaut is RNase H in this video.

Can anyone explain what is the difference between Dicer and Drosha (also Slicer/Pasha)?

Amazing explanation about interference mechanism thanks sir

Sir whether the double stranded rna is produced from the target sequence and it will cleave the same target mrna

should it actually be that coding and non-coding strand of DNA is being transcribed, so RNA can base pair not transcription of RNA?

have u cleared net jrf but very nicely and intelligently done

fabulous sir

RISC , (RNA induce silencing complex) not RNA interfering silencing complex

very helpful 😊

RNase III is a endonuclease enzyme and how it able to cut in the sides or in the end of the strands ?????

Thank you dude

Very nice

What are the intranuclear processing of a ds rna before leaving the nucleus??

Thanks sir ........

Sir does dsrna produce when the plant get infected with virus or its present already bin plant???

Sir, can u tell me if this can work in acellular organisms

What will happen to the passenger strand? whether its get degraded or not?

Which strand remained attached to the RISC complex if it would of been the antisense one then and only then we get an inhibition effect of target mRNA.. what if that strand is a sense strand.. then the whole process will be futile?.. plz answer the query.. it was a helpful video though..

nice!

Cloud you please do videos on MLS 4550 Clinical Immunohematology?

Sir Can you please recommend some molecular biology books.

bro you say that in miRNA after forming RISC there are two domains one is paz that attach with single stranded mRNA & another is pivi domain have RNase activity..OK in all type of so,sh,mi RNA strands are unwinds by helicase?? are some factors involved??

very useful thnks

doesn't the helicase come into play in mi RNA processing?? pls specify as I'm having doubt

Thank you so much!!!!! :)

After cleaving the mRNA , whats the fate of guide strand further??

How double stranded Rna is introduced

sir most of the plant viruses are single standed so what is the use of rna silencing in case of plant-virus system

what about the guide rna strand?? after cleaving the target rna strand what happens to the guide rna

This method use in Corona disease ??????

If so rna block the expression of m-RNA then how normal translational takes place

One requesting for make pi or Piwi rna

Could it potentially cure HIV in the future? RNAi seems to be able to tamper with RNA replication of viral RNA in cells.

dicer(RNase III) cleave ds RNA in pre mi RNA in cytoplasm and drasha cleave mi RNA in pri mi RNA form..

9.23 endonuclease...how??dicer is cutting from the end...then how?

sir I need online study of my Pg medical microbiology course