Southern Blotting | Basic Procedure | In Hindi

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#Southern #Blotting #BaaYo
Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labelled probe hybridization.Basically, the DNA fragments are separated on the basis of size during electrophoresis. Separated DNA fragments after transferring on nylon membrane, the desired DNA is detected using specific DNA probe that is complementary to the desired DNA.A hybridization probe is a short (100-500bp), single stranded DNA. The probes are labeled with a marker so that they can be detected after hybridization.
Procedure/ Steps
1. Restriction digest: by RE enzyme and amplification by PCR
2. Gel electrophoresis
3. Denaturation: Treating with NaOH
4. Blotting
5. Baking and Blocking with casein in BSA
6. Hybridization using labelled probes
7. Visualization by autoradiogram
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27 сен 2024

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Комментарии : 369

@BaaYo Год назад

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

@pronoobvines6178 4 года назад

Really 1st time in my life I found a madam with lots of concept and basic knowledge 😁😁😁. Sorry if I hurt anyone

@BaaYo 4 года назад

Thank you MR.

@Salmankhan-dx1do 8 месяцев назад

🤫

@anitathakur4514 4 месяца назад

Same here mam nhi hota to pta ni ky hota

@zeeshantayyabi1392 5 лет назад

A big big big thankkkk u ma'am.......u have helped out me with great difficulty in this topic.......hatsoff to ur explaination🙌🙌

@dilipsangam2091 4 года назад

Wonderful

@dilipsangam2091 4 года назад

Wonderful Presentation.

@rimjhimBedi 2 года назад

Thank God I've got to watch your vids. Otherwise I would have skipped these blotting related questions in exams. Thanks to you and ur amazing teaching skills that made me secure good marks.

@anamikabhardwaj5170 Год назад

Seriously last moment pe video dekh Rahi aapka. And it's awesome... thanks alott...ekdum crystal clear concept...😊😊

@BaaYo Год назад

Thank you

@kashifahmad287 4 года назад

thank you mam so muchhhhh from Pakistan. now my presentation is ready for tomarow.

@oishe3988 4 года назад

Tomorrow

@ammarsayal 2 года назад

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@kanikamalhotra7220 Год назад

Thanku so much mam graduation students needs teacher like you........thanku soo much this is very helpful video ❤

@BaaYo Год назад

Thanks a lot

@sreemotichatterjee4345 3 года назад

simply explained such a big concept. Thanks a lot ma'am for making the concept literally crystal clear.

@rajyagurukrupa7398 Год назад

Thank you so much for this explanation....you are truly amazing ma'am ❤️❤️ your short videos are much better than other 1 hour lactures...

@Euphoria321 Год назад

Love the way you deliver lecture❤ simple, elegant, full of basics❤ thankyou Madam!

@subhrajeetdash6226 5 лет назад

Thank you mam..I am from chemical engineering and I find it useful in my work too

@ph100k3 2 года назад

Thank you mam...For Very Easy Way teach us❤💯🙌...Huge thanks to you and your team😍😇

@BaaYo 2 года назад

Thanks a lot 😊

@muhammadosama1874 2 года назад

👏👏👏👏👏👏👏..u deserve best teacher award ....teacher of the RU-vid award

@fh19293 4 года назад

Thanx a lot for clearing this topic in such a beautifully explained manner😁😁😁...just a request, can u plz explain the topic of 'autoradiography'

@takini4918 2 года назад

Bdi aasani se concept clear ho jate hai aapki videos dekh kr. Dhanywad

@official.rajarshidutta 5 лет назад

Mam, I am so glad to see your channel grow. I hope you remember me. I have been your subscriber when this channel had hundred subscribers. I pray this channel hits a million.

@BaaYo 5 лет назад

Thank you Rajarshi :)

@sanju-bt8ss 3 года назад

Thank you so much mam

@priyanshipatel2484 4 года назад

Really a great explanation, it helped me a lot, thank you mam

@noumansaeed7853 3 года назад

Very interested and beneficial lecture about southern blotting Thanks such a great work

@satyabratatripathy1613 4 года назад

It's amazing mam.. Really it is very useful for graduation students... Ty so much mam for clearing the concepts of us

@rishuyadav9667 3 года назад

I have hardly seen teachers with such conceptual clearity and confidence like you. Warna aajkal to sab bs book se as it is copy paste krte h blindly. Also use of regional language is appreciated. 👍 Commendable.

@husnainaziz8623 3 года назад

Bravo, never learned these topics with such clarity. U seems a born teacher mam🤗

@sumitpatel868 5 месяцев назад

Daimmmmmnn , thank you so much 🥺🥺🥺 I was so worried about genomics, you explain so well and logically ♥️🥺

@parvinbegumaaraa2289 3 года назад

Mam, thanks a lot, I have little bit doubt, but get clarified after saw this video, one kind request, can U do the same in English for better understanding to all. Thank you mam. Nice explanation.

@maheklakkad9564 6 месяцев назад

Very clear concept , easy to understand, amazing way of teaching, mam thank you

@BaaYo 6 месяцев назад

Thank you. Please share and subscribe

@chintakayalpurnima1741 2 года назад

Hello ma'am. I love all your videos. I am myself a Faculty in University, but I love the way u explain. Beautiful

@jawadjoya3004 10 месяцев назад

Well informed and have concept after almost two years thank you mam

@sanchariguha5337 4 года назад

Mam this is really helpful , can you please also give us some links on written notes , like from where we can read this . It would be very helpful . Thank you again .

@nanavatijayesh8143 3 года назад

Your teaching technique is wonderful and makes direct digestible by mind

@vikrambiradar6411 2 года назад

Love you mam ... You saved my biotech exam paper from back log

@junaidaziz6933 11 месяцев назад

I am student of BS Microbiology And Molecular Genetics 🧬 😅 from University of Punjab Lahore Lahore. RU-vid is great teacher and this one mam too

@aishwaryawadkar2240 3 года назад

U r amazing.. The way U teach this biotech concepts is hilarious... Thankssssss alot mam.. U r best teacher 🙏🙏

@abhishekbbetageri6027 2 года назад

Huh? What is hilarious in that?? Ma'am is not cracking jokes. Btw, juz check the meaning of hilarious.

@knowmore2800 2 года назад

thank u mam u made me understand the concept easily and clearly.

@ubaidshaikh2153 Год назад

I just see your Vedios and write confidently in Exam. Thank you and God bless you!

@BaaYo Год назад

Thank you

@Maitri24 Год назад

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@pankajsaklani2343 5 лет назад

I am eagerly waiting for every video of you and it proves to be very helpful for me, god bless u

@riyarajput4117 Год назад

Thanks from my heart . Mam you explain it in a very easy way thank you so much h❤

@umeshrai9323 4 года назад

Very nice explanation. I appreciate your lacture. .now i feel easy on the topic southern bloating

@haleemasadia852 2 года назад

Thanks yeh thanks Dil ki gehrayon se

@simransadhwani5176 2 месяца назад

Wonderful explanation mam... really love it my concept is fully clear now

@twinkle1548 2 года назад

Wow!! Ma'am very nice explanation, literally have no words to explain. Big fat thank you!!!!💕

@sofiaadil7419 5 лет назад

Mam i wanna ask a question.....why you upload a video in a month...... please upload regular videos on biotechnology topics regularly, so we can get better benefits from you. Please Consider my Thought. Thank You Mam Lots of love from your Fan

@BaaYo 5 лет назад

Thanks for the feedback Technical Sofi. You are right, I have not been able to make videos as regularly as I should have. Got occupied in some other projects. But I am trying to make time for more videos and become regular, Hope I am able to meet the expectations soon.

@sofiaadil7419 5 лет назад

@@BaaYo Thank you Mam for your reply, and i hope the Baayo Family will increase rapidly with your hard work and also with our support.

@aksciencetutorial5702 4 года назад

Wow mam superb teaching mujhe aap ka jyda samjh ata hi you are great mam

@twicey422 2 года назад

mam why there is no "eastern blotting "techniq

@rajarshisen7543 4 года назад

Mam your explanation is superb and very clear. All your videos are too good mam. I am a very big fan of your teaching.... Thank you so much mam🙏🏻🙏🏻.

@researchease 4 года назад

Thanks and love from China. Simple and easy way me best explanation.

@Pharmacy891 2 года назад

Mam very very thanks from my heart .for understood in easy of this difficult topic

@proudtobeveterinarian6717 Год назад

Mam 😊 now I became huge fan of you ...

@aniketdas7803 Год назад

Very helpful, thank you mam ❤

@sultanmehmood5022 2 года назад

Thanx a lot, mam its so easy way explained, shall never b forgotten. I shall search for other blotting also

@shahbaloch4814 3 года назад

The amazing excellent teaching method easly understanding style it is very helpful thanks A lot madom 😍

@shreyaskulkarni5312 2 года назад

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@VetAnilturkiya 3 года назад

Ma'am I love your teaching style help full conceptual

@ektasharma4816 2 года назад

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@syedmedicose7477 2 года назад

excellent maam.... stay blessed

@NavjotKaur-sf9ie 4 года назад

very nicely explained...Keep it up👍👏

@Greensignal2022 2 года назад

lecturer and lecture both r awesome..hamara dhyan kidar gayahi nahi .video sey bahey😁

@PraveenKumar-bv2xe Год назад

Thank you mam for making this topic very simple and easy.

@kashwiagrawal9785 Год назад

A very good and clear explanation, your video helped me a lot...thank u so much

@tamanaah-u4g 9 месяцев назад

Thank you ma'am excellent 👍 teaching

@nidhi878 4 года назад

Thank you so much it is really very much helpful

@zaminali4937 Год назад

Amazing teaching skill

@honeypatel1860 2 года назад

Really a great explaintion, thank you mam 🤗

@ruchikumari1865 3 года назад

This video are very help for me... Thanks mam 👍

@simmianam7721 4 месяца назад

Awesome explanation... thank you so much ❤

@muhammadnazakat9416 3 года назад

Thank you mam nice to meet you.

@jaydeepdumadiya2464 3 года назад

Great ma'am... God bless you 🙏🙏🙏🙏🙏🙏🙏🙏

@youngcartoon7196 3 года назад

The way u explain it's very very very nice maam i m ur huge fan 🥰🥰

@mahmudulhasan-ws5jw 2 года назад

Thank you for clearing in such easy way

@preetsaroay 4 года назад

Thank You So Much Mam.. I love your way of explanation.... Thnku very much....🙏😘❤️

@ganga6102 3 года назад

Very very thank you ma'am for explain the difficult process. 🙏🙏🙏🙏🙏

@rekhakushwaha4744 4 года назад

Mam aap bhut acha padhti h thanku so much

@ShivPrakash 5 лет назад

Awesome keep doing best...👍👍👍

@BaaYo 5 лет назад

Thanks Shiv Prakash 😊

@prachijain8031 2 года назад

Very good explanation 👏👏👏👏

@Prince_sen_0001 Год назад

Best teacher ☺️ thanks dii🎉

@ramchandrasuthar2848 5 лет назад

Nice analysis mam

@SimplePharmaBasics 3 года назад

Excellent explanation ma'am 👍🤩✨

@easywaytolearn5782 Год назад

It's a amazing explanation lot of ty ma'am . ❤❤❤❤

@BaaYo Год назад

Thanks for liking

@crazycoins8923 3 года назад

Hats off mam👍🏻👍🏻👍🏻👍🏻thank you so much.

@shazmanimushtaq8359 Месяц назад

Very nice explanation

@Shamasimplelivingirl 3 года назад

Excellent explanation Ma'am 👍👍👍 Thank u so much 🥰

@PrabhakarKumar Год назад

Thank you mam 😊 for your easy explanation to this topic

@talhawaheed438 Год назад

Awesome explanation.... 👌

@vandhanavandhu8090 Год назад

What a combo beauty with brain 🌝

@sayambabu7365 3 года назад

Best one yet 🤟🤟..

@jaypalshiyal767 2 года назад

Thank you soo much maam ❤️

@SiblingsArtZone Год назад

thank you so much mam.. very helpful video

@mahimatomer8408 5 лет назад

Amazing video mam🙏

@Kb-cp3vk 2 года назад

Great ! Thank you ma'am

@sagarmali3250 3 года назад

Excellent Mam keep it up 💯

@sumangawariya1161 Год назад

Thanks you so much ma'am 🥰🥰🥰

@arjunt1453 3 года назад

Thank u Baayoo ♡

@KrishnaKumar25261 3 года назад

thank you mam for crystal clear concept.

@shatrudhangupta2163 4 года назад

Very good explanation for southern bloting.

@22mayurigadhave13 2 года назад

concept clear doctor

@aishwaryawadkar2240 9 месяцев назад

U r jst amazing.. Luv u mam❤

@Nomadicsumit 4 года назад

Really mam u r great and way of explanation is awesome ❣️

@Meenakshi08_x8e 3 года назад

If we directly use RNA then also we have to perform aagrose gel electrophoresis process?????

@Aditi_choudhary944 Год назад

Fruitful to me THANKS mam

@takini4918 2 года назад

Thank you so much ma'am.

@rubababbas8696 4 года назад

Thank you so much mam .... From Pakistan