In this demonstration cuvette is filled with chemical (for measurements 3/4 cuvette is enough). Some times when using with full cuvette, the chemical gets spilled into the cuvette holder. If the chemical is highly corrosive, it attacks the inside electronics and optics of the spectrophotometer. As a precaution, it is better not to use full cuvette chemicals and also better to use a cap for the cuvette (it is available). I have seen this problem before and sharing this with the students and the instructors.
impressive presentation ... for one & all new to spec's . now i want to see more vid's you've posted. by showing the lo - A end of the spec's ability to measure dilute solutions an answer to my question is had. i wanted to know this in order to test a tlc strip's ability to detect ug amounts of a cd. also found it interesting your method has you re-use the same cuvette. thanks too for the rundown of the prep'n of each flask with those iron reagents. this will call for some homework study to understand why this is done. and to learn what a test of the un-altered iron would have told you.
When it was said "Wipe the Glassware container down if you touch it with your hand," may I assume the comment was made to refer to an "ungloved' hand but touching with a "Clean Gloved Hand" is OK? I am new to this.
That Spectrovis thing is cool. Its alot better than the spectrophoto meter machine its biger and havier this one is simple and easy to use. It must be expansive. Thank you for the video:)
@@syedmbilal7741 There are several reasons your spectrophotometer could be larger. It likely depends on the type of source and the type of detector your particular instrument has. Diode array instruments are typically preferred for teaching instruments, as they have few moving parts and are quick and relatively cheap. Instruments containing monochromators are preferred for higher quality measurements (like those in a research lab).
good afternoon, good video. I can determine any colored salt with this technique? I want to analyze Au, Cu, among others. It is possible with this technique thank you
hi there, i want to ask you is it possible to measure the number of ferrous iron? I work with the bacteria which able to reduce ferric iron into ferrous iron in the anaerobic condition. And i have a difficulties to estimate the number of ferrous that produced. Because i thought that ferrous iron inside the medium which put in the anaerobic condition will oxidized directly when it's contact with oxygen in the aerobic condition. Is it wrong? thank you
+Kristin Widyasari Hi Kristin. You can measure ferrous ions by difference. You take an amount of your solution, you oxidize the iron ions you have in solution to Fe3+ and measure how total iron ions. After that you realize the purge procedure in another amount of your solution (the same, but another aliquot) and measure the total ferric ions without preoxidation of the iron ions (u can use a N2 or argon atmosphere). The difference is the amount of ferrous ions. Bye (PS.: Sorry my english. This language is not my native one)
Kristin Widyasari You're welcome, Kristin. You can use some electroanalytical method, too. Amperometry is a good choice, so you can measure the concentration of Fe2+ and Fe3+ ions in real time while your biological experiment is proceeding. Wish you to have a great day, too.
Yes, but you'll need to extract the iron from the food. Typically high iron foods like spinach work fairly well. The Harris Quantitative Analysis book has a procedure for such an analysis.
