Spore cleanup

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Mushroom Cult demonstrates rescuing germinated chestnut spores from an agar plate that has sporulating mold contamination. Why? What if this was your best plate from a rare spore print you only had access to one time. you better be ready. Practice when it doesn't matter so you are ready when it does.
Experimental Comentarty Contest!
Send your audio commentaries to mushroomcultpro@gmail.com
The best commentary will be added to the demonstration section of the video. Must be in mp3 format and no longer than 4 minutes and 20 seconds.
Submissions are due by January 25th
Get critical Myco-Ninjas!

Опубликовано:

21 окт 2024

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Комментарии : 69

@SirBoden 3 года назад

Move the contaminated lid once, slowly. Taking it on and off is fanning the dish.

@PuppetMasterdaath144 Год назад

'fanning the dish' is this CIA cryptic messaging or smt

@Kitopa 4 года назад

Very nice video, hope to see you post more soon. I would follow your process throughout the growth of your plates, grain, substrate, etc!

@mushroomcult 4 года назад

I am working on following the process of collecting a wild mushroom, cloning, cleaning and so on. It will take some time but it's on the way.

@hakasonma8588 Год назад

I just noticed you don’t really do air filter or SAB do you normally do it like this or just for the video? Looking to start agar plates but if I have them have some much equipment may just stick to injections even if slower lol

@kylewalter6714 3 года назад

That spray bottle would fit in the SAB much better than the bottle that came with the yellow spray top! Thanks for the tip!

@mushroomcult 2 года назад

glad to help

@righteousmonarch9499 3 года назад

This is the perfect video I was looking for thank you so much!

@mushroomcult 3 года назад

Glad it was helpful! let me know if you have questions

@dreadtrain2846 4 года назад

Use an SAB, don't say you don't need one. You need one if you don't have a flowhood, your bathroom is not sufficient. Make it super clear. There is enough misinformation out there without new peeps thinking a bathroom is the way to go. This is my biggest gripe heh.

@mushroomcult 4 года назад

thanks for your comment. I agree. Im working on a series for best practices. I am planing to be mush more clear on the cointent. I want to be a trustworthy resource. I am also wanting to push boundaries. many people in this community are stuck in a ritualistic and dogmatic devotion to a specific TEK without thinking about why.

@AaronAlso 3 года назад

A cheap 3x3 grow tent is exponentially better than a bathroom. I use a tent, SAB and a UV sterilizer for agar work.

@fishmut Год назад

Bathrooms usually the best place to grow mold to , wet damp conditions , not a place to do this work at all.

@daryushnadjafi6673 3 года назад

Thanks for the video, great info and straight to the point 👍. I'm subbed. For the first time did 12 no pour agar 3 weeks ago, in one jar there is a tiny miniscule black speck. To me it doesn't look like any of the contamination I've seen in videos. I'll keep an 👁 on it. Do you have any idea what it could be? It wasn't there in beginning, and I don't c it growing.

@mushroomcult 3 года назад

has the speck grown? it could just be a piece of black material

@daryushnadjafi6673 3 года назад

@@mushroomcult hi thx for your response. No the speck has not grown for a week sinds post. U think it's benign? Have no idea where it came from, I did a spore rig to no pour agar. Could it be a small cluster of spore?🍄🕉

@lukebrietzke8514 3 года назад

Well that was easy. Thanks for the info.

@mushroomcult 2 года назад

Glad it helped

@AaronAlso 3 года назад

The description is my nightmare scenario. This is why I keep two slants of all my best cultures, in a fridge.

@mushroomcult 3 года назад

exactly! I recommend throwing this away and not following my example. i just wanted to demonstrate the process.

@davids11131113 Год назад

I’ve tried save a few agar plates it never worked for me….probably beyond saving and contam had already spread extensively

@mushroomcult Год назад

Yah. This example is past when I would normally bother but it is good practice for when you have an emergency

@davids11131113 Год назад

@@mushroomcult One time I got in some agar dishes and 1 of the lids was cracked, I decided just to try it anyway for the hell of it I taped the crack with some package tape, and put some mycelium on it. Next day I noticed a bit of mold had showed up right under where the crack had been, so I opened it back up in still air box and cut out the mold spot then carefully wiped the rest of the agar with a paper towel I sprayed with some alcohol. It worked and no more mold showed up.

@AS-ll5nd 3 года назад

After pouring and sealing agar plates, how long should I wait before starting work with them? Would it be sensible to wait 3-4 days to see if any contamination from the agar technique is present?

@mushroomcult 3 года назад

that would be a good practice. as you get more secure in your technique you can use them as soon as they cool but set a couple to the side as blank controls.

@johnhacker6480 2 года назад

A tip for the next video, ur voice is a little bit too lound, lower it next time :)

@danjf1 2 года назад

Maybe write your sharpie notes on the plate NOT the cover. Small detail, but sometimes stuff happens. Don't like the bathroom non still box, non flow hood, etc...MT, ASCP

@mushroomcult 2 года назад

what do you think of this lab setup? ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-KmPHX1ZH-L0.html

@JavierFernandez01 2 года назад

Interesting stuff.

@jamegumb6625 3 года назад

I know nothing about mycology so don’t get frustrated at my ignorance but could you drop a small amount of bleach or alcohol just on the bacteria to kill it or would that kill the mycelia too?

@mushroomcult 3 года назад

It will probably kill both. This wouldn't be a good practice even if it works. The idea is to move the cleanest mycelium away from contamination. Antibiotics can be added to the Agar plates that can accomplish what you are thinking but it needs to be added before the plates are inoculated. Does that answer your question?

@jamegumb6625 3 года назад

@@mushroomcult one more question, I lost one of my weights on my pressure canner and I can only get 10 pounds or pressure for my grain spawn jars, can I still get proper sterilization?

@mushroomcult 3 года назад

@@jamegumb6625 you need to run it maybe 25 - 30 % longer. You could also put a wet towel over the weight to hold more pressure if you are able to monitor it.

@KevinLeRoy1 2 года назад

@@jamegumb6625 or tape a few coins on it till it wiggkes at the right pressure :)

@chrisbelanger6467 4 года назад

I like your style.. you a good person

@mushroomcult 4 года назад

I appreciate that!

@nachospopthe3rd564 2 года назад

I just chuck mine at the local trash pandas

@mushroomcult 2 года назад

I have to say it is quite delicious

@nachospopthe3rd564 2 года назад

@@mushroomcult they can have a lil agar as a treat lol

@zebraney5247 4 года назад

I also noticed you’re not taking from the leading edge

@mushroomcult 4 года назад

great observation. thanks for your comment!

@Christine.corneille 2 года назад

There are spores on the floor in the flat i rent. Two days after cleaning they come back all the time. Look like dust but it 'isn't.

@mushroomcult 2 года назад

exactly. a still air box is very helpful to keep your work clean

@Trenscendent 3 года назад

So you used the plate you were cooling the blade down in for the final inoculation and you didn't flame sterilize the blade use for the final inoculation?

@mushroomcult 3 года назад

i'll need to watch that again but i sterilize the blade, cool it in a clean plate before making a transfer. is that what you saw?

@Trenscendent 3 года назад

@@mushroomcult no, watch it from 7:00. You pull a new blade from it's packet but never flame sterilize it before using it. And it appears you use it on the same plate you were using to cool down previous blades.

@ClaydogJr 2 года назад

@@Trenscendent why would you flame sterilize a brand new blade

@Trenscendent 2 года назад

@@ClaydogJr because brand new blades aren't sterile, duh

@ClaydogJr 2 года назад

@@Trenscendent that’s a surgical blade they come sterile

@zebraney5247 4 года назад

Why don’t you flip the fish you’re xferring from? Also you shouldn’t have to open the dishes youre inoculating As far as you are, I like to only open one side of the dish. Just enough to get the wedge in. You should wipe your dish with alcohol after you pull your parafilm. I get what you’re saying about being gentle with the dish but it’s honestly not that big of a deal, I’ve dropped dishes with trich in them and still was able to clean them up no problem.

@mushroomcult 4 года назад

Are you saying you would do the transfer from an upside down dish? I'll need to try this and see how it feels. I like your comments about the fine details. ill watch the video again and see if i see whet you are seeing. Thanks a lot!

@zebraney5247 4 года назад

Dish, not fish lol. But yes, your multi spore dish should be upside down imo, otherwise it’s ripe for more contams on your clean culture you’re about to xfer.

@ancestralhypnosis 3 года назад

@@zebraney5247 i was told that the reason we flip lids dishes is to avoid condensation

@zuul902 3 года назад

your videos audio is always so quiet...

@mushroomcult 3 года назад

I only want to speak to people who listen carefully.

@zuul902 3 года назад

@@mushroomcult 🤣💯

@jaimedelgado7529 3 года назад

hi, beginner here. very similar situation. could u explain why it's not just easier to go with a Qtip and some H202 and clean up the mold ? I really like to save my clean plates as they are a pain to make. I have a cat (litter box), mold on some walls and an indoor grow op (mildew, botrytis - these things happen) so if I do what u did I'm risking the same thing happening. like I said tho, I'm only a beginner trying to see what works best for my situation. thanx

@mushroomcult 3 года назад

trying to remove mold will cause spores to spread all over the plate and in the air. you always want to move the clean mycelium away from the contamination on to an new plate. check out my video on the no pour agar technique. it will help you make agar plates without the hassle. does this help?

@jaimedelgado7529 3 года назад

@@mushroomcult I've actually seen the video you recommended. instead of using tiny jars (which I don't have) can I pour the agar on contaminated petri dishes instead, put the lids on the dishes and PC the heck out of them ? I think theres no problem, my fear is the water/humidity go inside the plates. you know yourself these lids aren't very snug