Tecan's Magellan Microplate Reader Software - Creating a Method with Standard Curve Analysis

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This video describes how to create a simple method file in Tecan's Magellan software that will measure samples and derive concentrations for the samples using a standard curve. The method created here is relevant for use in ELISA assays, Protein & Nucleic Acid quantitation, or other assays where a standard curve is used. This video content does not contain instructions on how to optimize the measurement settings of your microplate reader.
Please return to this channel in the future or subscribe in order to be notified when new content is added. Links to more advanced Magellan concepts and tips for optimizing settings for Absorbance, Fluorescence, and Luminescence detection will be added soon.
The information provided in this video is not a substitute for official training from the manufacturer.
Magellan - How to run a method, collect and analyze data with a standard curve • Tecan's Magellan Micro...

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9 мар 2016

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Комментарии : 10

@cathyschannel28 Год назад

how can we set up two plates--one is for standard curve and another one is for sample plate?

@arpitkhanna3039 3 года назад

Thanks for the information its really helpful I have a question suppose i have generated a kinetic read of 90 mins data wd basic protocol now i want it to export it in a column format( like all A1 wells time points together) not in a plate map manner Can we do it...? Or before read only we have to do setup? I tried but its always gets exported to excel in plate format😊

@videotutorialsforscientifi3584 3 года назад

Sorry for the very delayed response. When editing your method, navigate to the screen where you can edit settings such as Data handling and select “data export”. In the data export window select the “export options” button and pick the export option “result” called Table(data in rows). This will export information for each well across the spreadsheet in rows so that each column will contain a specific type of data you are exporting such as, raw, mean, concentration, etc.

@videotutorialsforscientifi3584 2 года назад

Hi I apologize for never replying to you. I should have turned off comments because I am not monitoring these videos for questions. Either way, here is an answer to anyone else who has a similar question. As you are creating a method or reviewing data that has been collected, there are settings under "Data Export" where the format of the exported data can be driven into matrix, column, or row formats. You will find these in Data Export, "Export Options" visible in the video starting @10:26. To have your data appear with the wells running down the left side of the spreadsheet and columns of data types running across the top, select "Table(well data in rows). Thx

@CaaaroM 4 года назад

Hi! I am using a Tecan Sunrise microplate reader with the Magellan software for doing an standard curve but I have a problem. Whenever I want to run my curve, the machine says that I don't have enough base points... so, what I tried to do was a blank reduction (I am not sure if this was what I supposed to do but I gave it a chance) but I do not have the automatic option in the transformation section to do this. I tried to fix this by putting the formula (x-BL1) directly but it doesn't seem to be working. Can you help me with this please? Thanks!

@videotutorialsforscientifi3584 4 года назад

please go ahead and email me your magellan method directly to rcavicke@roadrunner.com and I will take a look. Because this is not an official Tecan site, I have to take your inquiry to my personal email address. Thanks for understanding.

@videotutorialsforscientifi3584 2 года назад

As long as you have defined at least two wells with the identifier called ST1_1 and ST1_2, and valid data points are collected for these wells, the software can plot a curve. Once you read the plate, if all of the data points for your standards are "OVER" , or if only one standard has a valid data value and the rest are "OVER", and beyond the OD range of the instrument, it will not be able to plot any points you'll get an error that there are not enough base points.

@ZeGZG 4 года назад

ChroMate 4300 PC controlled ELISA microplate reader Measures Absorbance of 96 wells in approximately 12 seconds User-friendly intuitive software Automatically positions plate, blanks, reads and calculates results Uses standard microwell plates for all reactions open system Four filter model (405 nm - 630 nm) Optional 6 filter VIS model (405 nm - 630 nm) I need a definition ChroMate 4300