Тёмный
No video :(

The Spectrophotometer: A demo and practice experiment 

BioNetwork
Подписаться 83 тыс.
Просмотров 740 тыс.
50% 1

The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light.
In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring.
Included in the video is a short animation that illustrates the inner workings of a spectrophotometer.
Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video.
Practice using a virtual spec www.ncbionetwo...

Опубликовано:

 

25 авг 2024

Поделиться:

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист
Посмотреть позже
Комментарии : 147   
@wheresjohn1944
@wheresjohn1944 8 лет назад
very relaxing review before a lab practical
@fariashinshin1041
@fariashinshin1041 2 года назад
Exactly
@thanushk
@thanushk Год назад
Exactly
@walsh0
@walsh0 11 месяцев назад
Exactly
@randomdude4360
@randomdude4360 10 месяцев назад
Exactly
@HiddenTalent_07
@HiddenTalent_07 8 месяцев назад
Precisely
@feminico2613
@feminico2613 3 месяца назад
bsmls 1b was here
@achlys7290
@achlys7290 3 месяца назад
wala tayong ganto sa school scamm
@BrennanDemarest
@BrennanDemarest 9 лет назад
Excellent video. Clear cut and well filmed.
@telracsazyl1243
@telracsazyl1243 3 года назад
no cap. this channel goated
@amailyakter6589
@amailyakter6589 3 года назад
Thank you so much for such an effective demo, I got the idea of how to use this machine
@athiramalukilikood6075
@athiramalukilikood6075 5 лет назад
Thankyou so much, today is my practical examination, and it was really really useful one😍
@jamalfareedi996
@jamalfareedi996 3 года назад
One of the best review of Spectrophotometer in lab
@theoryofeverything4237
@theoryofeverything4237 3 года назад
very nicely explained and the content is so smooth and crisp :)
@binbinky78
@binbinky78 12 лет назад
best explanation out there on how to use it. Thank you!
@tinaishemachida9798
@tinaishemachida9798 Год назад
could have never found a better explanation than this yohhhh!
@vahidmirkhani
@vahidmirkhani 5 лет назад
6:07 What does this plot mean? What's our interpretation? As you can see, the absorbance is relatively high for wavelengths below 600, but 600 above is not absorbed and hence, transmitted. So the photons reaching your eyes from going through this solution, mostly have wavelengths between 600 and above and hence, the red color. I hope this helped a bit.
@Teekay_ZA
@Teekay_ZA 5 лет назад
It helped a lot! Thank you
@noork.631
@noork.631 2 года назад
Thank u soo much
@leventnergiz
@leventnergiz 2 года назад
Thank you for your remarkable conclusion. it meant a lot to me.
@ChadCarney-hu3du
@ChadCarney-hu3du 7 лет назад
Now I have a way to measure if an increase/decrease of algae occurred in a sample of gymnodinium!
@davidmonroy3965
@davidmonroy3965 7 лет назад
How do you determine the wavelength that should be used? can it be a constant, and keep it the same for all my samples?
@darksector1389
@darksector1389 3 года назад
I'm assuming they are using a hydrogen source light where it has a constant wavelength when it enters the spectrometer. Then use this equation: d sin(theta) = m (lambda), where d = grating distance (which is written on device), m = 2 (using Balmer series for visible wavelengths). Then you get four discrete wavelengths of: 410 nm, 434 nm, 485 nm, 656 nm which are the spectrum of hydrogen emission.
@kusalseven3563
@kusalseven3563 2 года назад
Imagine working in a lab full of these retro style equipments 😌
@The_Bees_Knees93
@The_Bees_Knees93 12 лет назад
have a bio lab practical tomorrow, easily learned how to use it more by watching this video. thanks!!!! :)
@cophernicus510
@cophernicus510 2 года назад
That intro music was fire 🔥
@thainguyen5553
@thainguyen5553 11 лет назад
That's awesome! It would help me a lot for my experiment next week :D
@oldboy4782
@oldboy4782 6 лет назад
Relaxing demonstration with fine video shot
@rrbuff1960
@rrbuff1960 11 лет назад
I am not totally sure of your question here. The sample concentration is irrelevant for this activity. If you plot wavelength on x axis (380, 405, 430 nm....... ) versus the measured absorbance on the y axis you will find the wavelength of maximum absorbance, called lambda max. Each color has its own lambda max. That is the goal for this particular lab.
@OluwatoyinAdelusi
@OluwatoyinAdelusi 12 лет назад
You just saved me from failing a bio lab practical! Thank you so much!! :D
@telracsazyl1243
@telracsazyl1243 3 года назад
@@sasmitvaidya3594 its 8 bro
@ibby_h5574
@ibby_h5574 3 года назад
@@telracsazyl1243 was 6 at the time he commented
@josephbenobasa
@josephbenobasa 10 лет назад
This would come in handy for my lab tomorrow
@bennettegaper4344
@bennettegaper4344 5 месяцев назад
Thank you so much
@A_NILAVIDILAUTAMI
@A_NILAVIDILAUTAMI 2 года назад
Hi, this is a very interesting video and easy to understand. I'm currently a second year pharmacy student, and I am learning about Spectrophotometer. Can I use this video for my college project? I'll give BioNetwork some credit or acknowledgement in my video. Thank you so much, stay safe and healthy!
@NCBioNetwork
@NCBioNetwork 2 года назад
Yes, you are welcome to use the video as part of your project. Please provide the appropriate reference / citation to this video. Thank you!
@user-uj2le6bc5v
@user-uj2le6bc5v 3 года назад
what is the purpose of using blank solution?
@streaminggames9186
@streaminggames9186 8 лет назад
nice video helped me to learn more
@user-tm8up3db2d
@user-tm8up3db2d Месяц назад
Very good story ❤❤🌹
@louisegabrielbotastas9583
@louisegabrielbotastas9583 Год назад
Bery helpul in our chemistry class
@celticbard9313
@celticbard9313 8 лет назад
Awesome job explaining this, thanks.
@RedGoddesscom
@RedGoddesscom 12 лет назад
Thank you for upload. The explanation how it works is very good!
@rajput9s764
@rajput9s764 5 лет назад
Awesome and very useful😊😊
@charleskingsley4290
@charleskingsley4290 11 лет назад
This video was very helpful to me. Thank you so much for the video
@johnfernandes9004
@johnfernandes9004 10 лет назад
THANK YOU SO MUCH
@Naveenbabuborugadda
@Naveenbabuborugadda 9 месяцев назад
What is the blank solution used here?
@Emotions_of_chemicals
@Emotions_of_chemicals 8 лет назад
nice explanation...thanks bio network..
@kevingp12
@kevingp12 11 лет назад
I have a practical too on monday!!!
@vinhluong3336
@vinhluong3336 2 года назад
Why does transmittance of a blank have to be adjusted to 100% before recording the transmittance (or absorbance) of sample at any wavelength?
@NeuralManifolds
@NeuralManifolds 7 лет назад
At 2:07 what adjustment does the spectrophotometer makes inside when Transmittance is set to 100% while blanking ? what is behind that 100% transmittance knob?
@dr.hebasabry1521
@dr.hebasabry1521 4 года назад
Same question I need an explanation please
@vincaalkaloids6732
@vincaalkaloids6732 8 лет назад
Thank you very much.
@kiribundi
@kiribundi 11 лет назад
Yes, if your concentration is higher, your absorbance will consequently be higher
@HiddenTalent_07
@HiddenTalent_07 8 месяцев назад
Idk why I find this video so peaceful ahaha
@laraibfatima1643
@laraibfatima1643 Год назад
Sir I have a question ⁉️ 1.If you tell the transmitted light in %....then absorbed light must be in %......but you taught absorbed in (nm) and transmitted in % 2..if 10% is transmitted then 90% should be absorbed or used anywhere else?
@allroundergamer1738
@allroundergamer1738 5 лет назад
Thank you 🎈
@mapalojohnchanda4107
@mapalojohnchanda4107 Год назад
Absorbance spectrum for red food colouring
@munkhundarga9081
@munkhundarga9081 10 месяцев назад
VEry nice bro
@abdullahdar2527
@abdullahdar2527 Год назад
Even the man looks like Animated AI robot
@arbazkohati747
@arbazkohati747 7 лет назад
tkanks for demo and practice exp
@infomatics315
@infomatics315 6 месяцев назад
Can this be used for analysis of phosphorus content in plants
@Cosmicvisionz
@Cosmicvisionz 2 года назад
How can we calibrate spectrophotometer for lower concentrations of iron? Like in 1-5 PPb.
@nicolelevy219
@nicolelevy219 5 лет назад
Excellent
@Sluo1947
@Sluo1947 12 лет назад
that was beautiful :')
@shahdragb9062
@shahdragb9062 2 года назад
well explained
@FeliciaAdedotun
@FeliciaAdedotun 11 лет назад
Very helpful....thanks
@sanofarnisha5899
@sanofarnisha5899 6 лет назад
Thanks a lot
@TheViewer318
@TheViewer318 10 лет назад
Hye.. I have a question. If this is for adrenaline test in saliva, how could it help..? the output are all numbers on transmissions and absorbency. the aim to test the adrenaline is to detect stress, and through this test, how could it help? Thank u in advance.
@Dai_rui
@Dai_rui 3 года назад
Hey are we increasing the wave length?
@aurynhs196
@aurynhs196 5 лет назад
can you find starch concentration using uv spectrophotometer?
@Mobashar61
@Mobashar61 8 месяцев назад
Nice❤
@woosungchoo3282
@woosungchoo3282 2 года назад
lol why do you increse wavelenth by 25 each time just partice?
@anisarashid1135
@anisarashid1135 5 лет назад
why it's adjusted to 100% T every time wavelength is changed?
@deepakchoudhary9146
@deepakchoudhary9146 3 года назад
Watching in online practical )
@hezakadoo6514
@hezakadoo6514 2 года назад
The soundtrack tho 👌
@temesgendeka4094
@temesgendeka4094 3 месяца назад
Can you apload another video for polyvinyl alcohol
@bhartiandana8314
@bhartiandana8314 3 года назад
It helps alot 👍
@gianpd1834
@gianpd1834 8 лет назад
someone know why they didn't make transmittance vs wavelengh tab?
@debjitmukherjee8494
@debjitmukherjee8494 11 лет назад
the video was very very helpful to me.,i request maintenance guide about the spectrometer video present it.
@snwbubble
@snwbubble 4 года назад
why so serious?
@nayanpanchal5538
@nayanpanchal5538 3 года назад
How we evaluate concentration
@Mauberetl
@Mauberetl 12 лет назад
Can you tell me, what is the concentration of your samples, which you inserted into the sample compartment? I'm confused, varying wavelengths when you use?
@jennifermiranda6998
@jennifermiranda6998 Год назад
I was thinking the same thing. If the dilutions are made of the same solution, then why vary the wavelength? That and/or molar absorptivity should be the same, unless again, the samples are different. Also, no need to place the blank every time you run a sample made of the same solution (but only being diluted). Blank should be read again if you are running a different solution sample. Again, that's just me. I think they did these customizations for demonstration purposes.
@Mrsemanmahmoudm
@Mrsemanmahmoudm 12 лет назад
so good
@serena3
@serena3 3 года назад
What will happen if you forget to wipe the cuvette before putting it in the spectrophotometer?
@tamileditingstudios9325
@tamileditingstudios9325 2 года назад
It will show wrong value.
@shyamkumar-cz5ow
@shyamkumar-cz5ow 11 лет назад
thats awesome! but i wanna know why are we changing wavelength of light for same samples?
@crimsonwood5264
@crimsonwood5264 4 года назад
To get a different absorbance values and use it to plot your graph because in each wavelength length the value of absorbance will change.
@talibmuzafar8836
@talibmuzafar8836 7 лет назад
how to calibrate the spectrophotometer?
@jiteshyadav3466
@jiteshyadav3466 4 года назад
Sir I want to check copper purity 99.99%. How to check this process.
@peterluxus7382
@peterluxus7382 5 лет назад
you don't this manually in 2019???
@atishkumar708
@atishkumar708 3 года назад
purpose of blank?
@chiomaobunadike3313
@chiomaobunadike3313 2 года назад
I still don’t understand what we would do with these values
@dailygrow6961
@dailygrow6961 3 года назад
Fascinating to see. Build one from paper on my channel 😂
@aashinabhatia1612
@aashinabhatia1612 4 года назад
What's the purpose of blank here
@raqiamalik9419
@raqiamalik9419 2 года назад
hi, why you test the sample again can you reply me
@user-gi1wr7ni4s
@user-gi1wr7ni4s 3 месяца назад
To measure absorbance and transmission at different wavelength
@anannaone
@anannaone 4 месяца назад
@pinkyy3
@pinkyy3 11 лет назад
Me too!! Ahhhh :S good luck!!
@jayeelli3906
@jayeelli3906 3 года назад
how to analyze an unknown substance using spectrophotometry?
@ishanilahiri7387
@ishanilahiri7387 3 года назад
If you know what kind your unknown substance is like is it a protein or a reducing sugar, then prepare a standard curve of the substance (of known concentration) closest to your unknown substance, and then map it out.
@jayeelli3906
@jayeelli3906 3 года назад
@@ishanilahiri7387 hi thank you. i love u so much
@Mauberetl
@Mauberetl 12 лет назад
nice..
@Digital_Guidance
@Digital_Guidance Год назад
Show calculation part
@Zept41
@Zept41 6 лет назад
What song is this ? Dammmn
@miklaszjay
@miklaszjay 7 лет назад
Looks like he knows what he's doing, but his data sheet is kinda sketchy. He's going to run into trouble when he's making the plot...
@fss1704
@fss1704 6 лет назад
miklaszj non automatic spectrometer in 2018? You've gotta be kidding me, my DIY spectrometer cost $50 and does auto sampling directly to my cellphone screen, seriously, all of that user interaction and sample being completely taken in a different direction each time should make your results have a mount everest of error.
@ethicalrevolution3294
@ethicalrevolution3294 4 года назад
@@fss1704 What is your phone spectrometer like?
@zodd0001
@zodd0001 6 лет назад
It takes a lot of time make just one complete spectrum. It must be an old version of spectrophotometer or just a didactic one.
@fss1704
@fss1704 6 лет назад
zodd0001 fking yeah, i build an automatic spectrophotometer in my bedroom pretty much the size of a cigarette box that can connect to my cellphone via non standard bitbanged infrared signals using an adaptor in the audio jack to send commands to the spec microcontroller and receive the digital data via the jack microphone input, i spend 50usd in it and the result is pretty much high grade lab quality.
@fss1704
@fss1704 6 лет назад
Theoretically you can do the read using an USB sound adaptor and bypass the input cap on the microphone, then use the sound output of a moving loudspeaker with a prism glued on top to filter the light, and a photodiode in the mic input to read the level of absorbance on each wavelenght.
@yxz458
@yxz458 8 лет назад
u don't have to set the transmittance to zero before putting in the blank, since the trans/abs will be changed to either 0 or 100 after putting in the blank solution. this extra step is confusing and unnecessary...
@fss1704
@fss1704 6 лет назад
Joanna Zhang i made a diy version for 50usd and it can plot the results in the screen of my cellphone, and it's a LOT more precise than this "remove x and exchange it by z" error monstruosity. Good fking luck correlating all of this data points from a moving sample.
@alvinlisyandi8541
@alvinlisyandi8541 3 года назад
Ilmunya sangat bermanfaat tapi sayang saya tidak bisa bahasa Inggris
@oldboy4782
@oldboy4782 6 лет назад
I almost gonna sleep
@t.s.m7116
@t.s.m7116 4 года назад
استمر حبي
@ezg_
@ezg_ 8 месяцев назад
عاش مال تقنيه 😂😂
@jordanmellis314
@jordanmellis314 6 лет назад
Why did we have to set the wavelength to specifically 380 nm?
@gatovillano7009
@gatovillano7009 5 лет назад
My god, that machine looks older than me =P
@brianvandenberg4467
@brianvandenberg4467 6 лет назад
6:07 Organic biotags charging for fun
@mataralhammadi3268
@mataralhammadi3268 4 года назад
1:20 looks like the guy from Jurassic Park
@Salkebulan
@Salkebulan 11 лет назад
Score !
@indrarayendra5530
@indrarayendra5530 3 года назад
Dewissengi aga kasi bettuanna
@user-jm5wz5yr6k
@user-jm5wz5yr6k 4 года назад
جميل
@ulfataabid2809
@ulfataabid2809 3 года назад
I have a qstion sir .. can u please help me sir ..... Make a report about Spectrophotomter from zero to 100... I don't know sir what I write in this assignment .... Becz of covid I never seen this practical .... How can I write .. can u plz help me sir so I can learn something about this and I can submit my assignment... Waiting for ur rply sir
@rebeccalames2495
@rebeccalames2495 3 года назад
bakit pa kasi online class
Далее
UV Visible Spectrophotometer
14:19
Просмотров 202 тыс.
Using a Micropipette - University of Leicester
8:49
Spectrophotometry and Beer's Law
6:25
Просмотров 524 тыс.
Using a spectrophotometer
3:32
Просмотров 73 тыс.
UV Vis spectroscopy 🌟
9:18
Просмотров 578 тыс.
How do you use a Micropipette? A step-by-step guide!
6:09
Spectrophotometry Explained For Beginners
4:39
Просмотров 74 тыс.
Using a pH Meter
4:48
Просмотров 776 тыс.
A better description of resonance
12:37
Просмотров 1,4 млн
Spectrophotometric Determination of Iron
14:05
Просмотров 217 тыс.