Thin Layer Chromatography and Retention Factor 

Yep, and it could also be alumni for TLC which is slightly less polar than silica. Anyway what confuses me the most about TLC is that the stationary phase is highly polar, and for our substances to travel up the plate they need to be soluble by the mobile phase, which could be polar or non-polar, so if our mobile phase is non-polar and the substance is polar, then it will not be dissolved by the mobile phase and it will have high attraction to the stationary phase so it really won't move much or at all, however if our substance is non-polar, and our mobile phase is polar meaning it still won't dissolve the substance, then that means the substance doesn't have attraction to either of the phases, so what happens? Does our retention factor stay at or close to 0 as well in this case?

Your view is too narrow. I've took IB Bio and AP Chem and both courses cover this topic.

@@lynny7868 ohh

We are addressing this topic in Organic Chemistry Lab now. I do not remember this being taught in Bio I or II. It would be interesting to see what unit in Bio you were taught.

@@SolaScriptura49 we are studying this in college in biology chapter 1of first year and chapter name is cell structure and function. This is in the topic of techniques for studying cell structure

Very interesting, I do not remember that being a topic in bio I, what a great introduction though!! We just finished recrystallization and are now in solubility in orgo. I would say this is used both in bio and chemistry.

Hope so bro you are clear now with this point after two years 😂..

Hope so you are clear now with this question after 4 years😂😂