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Thin layer chromatography (TLC) principle explained 

Shomu's Biology
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This lecture explains about the thin layer chromatography technique. TLC principle is well explained with example. Thin layer chromatography is the easy method to separate different types of chemical molecules from each other with the help of thin layer chromatography plate also known as the TLC plate. Thin layer chromatography separates molecules based on the differential movement rate in the tlc stationary phase which is calculated with the retention factor or Rf value.
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Thank you for watching the video lecture on the thin layer chromatography or tlc.

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1 июн 2024

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Комментарии : 474   
@saitejaediguttasaiteja8555
@saitejaediguttasaiteja8555 6 лет назад
Thank you very much sir, I got 94 th in CSIR JRF without coaching, I followed your vedios, really God will give great blessings to you sir.
@musicaddictedgirl3935
@musicaddictedgirl3935 2 года назад
Hii I m also preparing for CSIR net can I get notes
@bellam3173
@bellam3173 5 лет назад
Harvard needs to hire you as their professor! My professors in America are not as good as you! They don't explain the principles as you do! Good job!
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Thank you so much for appreciating my efforts
@anand-uk8ek
@anand-uk8ek 5 лет назад
Dude seriously....U must be kidding me..Huh?
@artishettiwar1800
@artishettiwar1800 2 года назад
@@shomusbiologyofficial 3333333333333333333333333333333333333333333333333333333333333333333333333
@mercymunkho5344
@mercymunkho5344 2 года назад
@@shomusbiologyofficial l)
@pradeepm7298
@pradeepm7298 2 года назад
Hlo I'm following from 3yrs ...your videos was very helpful for my degree biochemistry.......now I'm graduated with 75% thank you so much sir
@anmolgulati27
@anmolgulati27 6 лет назад
Thank you so much for such a simple and very informative video I have my exam on 30 and I will be able to understand all the chromatographic techniques from your lectures
@yashsuryawanshi6107
@yashsuryawanshi6107 3 года назад
I was not able to understand this concept for a week, But then I met Shomu sir in this video and Voila....It is fitted in my mind the easiest way. Thank you, Sir!
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@kapilmehta3976
@kapilmehta3976 5 лет назад
what a explanation sir I always watch u r video before my exam and my all concept is clear due to u r video thank u so much sir👌👌👌👌🙏🙏🙏
@jalilkhan1897
@jalilkhan1897 5 лет назад
I was worried about my paper Biochemical techniques which is going to be held tomorrow... Then i came to ur channel n saw all topics related to biochemistry here.. thanku so much sir. Allah bless u
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Thank you. Glad you liked my lectures
@Quotila0
@Quotila0 5 лет назад
My paper is after few days.
@chinmayeethakur5223
@chinmayeethakur5223 Год назад
These videos are like saviours ..I am brushing my Biotech syllabus , that I studied 7 years back ..this is helping me
@shomusbiologyofficial
@shomusbiologyofficial Год назад
All the best
@thecurious2825
@thecurious2825 5 лет назад
very easy wording,nice communication skills....great job,keep it up
@nenavathkaveri2422
@nenavathkaveri2422 3 года назад
Great work. Amazing ..Thank you sir Easy understanding each and everything..
@NehaSBaxla
@NehaSBaxla 6 лет назад
somu sir you are too great 🤗😊you r my saviour for all my exams 😄
@chejarlamounika6759
@chejarlamounika6759 2 года назад
Tq sir, very clear and easy way of understanding the concepts , The way of teaching u did was fantastic sir, thanks a lot.
@anaghathomas7411
@anaghathomas7411 5 лет назад
Good class sir.. Ur class is really informative. Thank u
@Jasmine_Jaiswal
@Jasmine_Jaiswal 5 лет назад
Thanks guru ji💞...you are the only one who helped me alot in exams since 1st sem till now🙏...
@Virat-rq7qf
@Virat-rq7qf 2 года назад
Tuje samj me aaya?
@ishalingayat3909
@ishalingayat3909 5 лет назад
Blesss u ....U r doing a great Job.....Go ahead 👍
@paridhopavkar9874
@paridhopavkar9874 6 лет назад
Thanks alot Sir.. It's helping me a lot.. 😊🙏
@jemimalarrson8756
@jemimalarrson8756 2 года назад
i always used to watch ur video's before my exams and it's really useful and the way u taught us is awesome sis tnk u so much❤
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
You're welcome. Glad you like it
@idrisd52
@idrisd52 6 лет назад
Awesome explanation sir...Thankyou
@bhagyajitdas1498
@bhagyajitdas1498 6 лет назад
Thank you sir for your explaination.. helped me on my exam....both TLC and ion exchange!
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+bhagyajit das glad it helped you
@learneasy8226
@learneasy8226 4 года назад
i almost forget the practical that i had done in my grad. thanku to remind me.
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome
@ucdiaries8064
@ucdiaries8064 5 лет назад
Extordinary..awesome..mind blowing sir
@nusrataane1488
@nusrataane1488 4 года назад
Sir wonderful explanation sir..can you plz describe mushroom cultivation compose, materials used in composing and different formulation used in composing , compost preparation and its methods...it will be your most kindness
@sridivyaaluri1215
@sridivyaaluri1215 3 года назад
Wow sir ur explanation is very clear and very good. U explained in such a way that I need not read it again anymore. Thank you very much 👏
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@prashanthraju4013
@prashanthraju4013 6 лет назад
Superb explanation. Tq very much
@vishwaskashyap308
@vishwaskashyap308 6 лет назад
Excellent demonstration
@swapnilombale4329
@swapnilombale4329 5 лет назад
It's qualitative...technique becoz one can identify the substance presence in mixture with the help of Rf value!!😊
@shujauddin5054
@shujauddin5054 6 лет назад
ThanQ sir...simple method say explain kiya Gaya hay..I know every one pick this lec
@achinidesilva591
@achinidesilva591 Год назад
I rarely post comments. But I really want to thank you for these lectures. Not only this video, I usually watch your videos whenever I can't understand a topic and I have never been disappointed. Thank you very much sir. By the way, I am watching this after five years of uploading but still it is thousand times better than my professor's explanation
@shomusbiologyofficial
@shomusbiologyofficial Год назад
Thank you so much for appreciating my efforts
@you-lf2uj
@you-lf2uj 2 года назад
Our teacher teaches us the principles for almost 2 months but still unable to get the grasp of it making me confuse everytime. Your teaching is clear and simple and I'm very thankful since I was having a hard time, may god bless you
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
Thank you so much for appreciating my efforts
@archna6436
@archna6436 6 лет назад
Thanks sir. you really enhance my understanding on the whole chromatography chapter.
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+archna that's nice to know
@Natureloverkerala
@Natureloverkerala 6 лет назад
Thank u sir.... excellent class
@ashwinipatil2581
@ashwinipatil2581 4 года назад
♥️Ur explaination is just osum yar nth is there that u cant explain ur very perfect in explaination 😇 in easy & understanding way ur all videos. Hlp me allot 😊tq so much bcz of u i scored hight marks in exam 😊tq onces again 😊
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Thank you so much for appreciating my efforts
@patige3930
@patige3930 5 лет назад
very much helpful brother keep it up👍
@mutaheramutahera5824
@mutaheramutahera5824 6 лет назад
I like sir ur explanations. Please Try to give like this other topics.
@BMAnahi
@BMAnahi 6 лет назад
Thanks man! Very Usefull video. From Colombia. :)
@durbadalgoswami
@durbadalgoswami 5 лет назад
Kindly share the standard Rf values of amino acids in TLC with Phenol water as the solvent medium It would be of great help
@nk714
@nk714 4 года назад
Thanks to explain in easy language......I also explained my friends easily
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome
@ChemistryEdu3
@ChemistryEdu3 6 лет назад
this is informative thank you
@jayantidas6930
@jayantidas6930 5 лет назад
Wonderful....well explained sir...Thank you so much sir...it is very helpful for me...Thank you...And I want to tell you that I regularly attend your videos before I start a new chapter....
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@ot-rp5tj
@ot-rp5tj Год назад
Very beautifully explained
@kiranbadiger6165
@kiranbadiger6165 4 года назад
TQ u Bhai ....... This concept of chromatography contains 30marks in our examination.........
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Glad to hear that you're getting benefit from my lectures
@harshoza1399
@harshoza1399 7 лет назад
Enjoyed your Lecture sir. Can you please make some videos related to SOS DNA repair mechanism and Centrifugation. Your Videos are great. Thanks for being a Long distance teacher.
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+Harsh Oza Okay. I will keep that in my mind
@DanishKhan-cq1dg
@DanishKhan-cq1dg 6 лет назад
Tomorrow is my paper and i had this chromatography topic.. I was going to drop this topic.. But MAN you saved my day...Tysm❤
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+Danish Drop thank you. Glad you liked my lectures. Please subscribe and share
@ghumketuovertheindia848
@ghumketuovertheindia848 6 лет назад
Such a nice explanation
@nyanrapier4952
@nyanrapier4952 7 лет назад
We were just about to start using this principle in our lab. Good timing and great video.
@shomusbiologyofficial
@shomusbiologyofficial 7 лет назад
+Nyan Rapier hope I can help
@loverajput1982
@loverajput1982 4 года назад
Brilliant teacher .......👍👍👍👍 Provide us concentrated knowledge🕵‍♂.....
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Glad to hear that you're getting benefit from my lectures
@sulemankhanvirologist5015
@sulemankhanvirologist5015 5 лет назад
Respect sir...i learn alot of knowledge... in Microbiology...immunology virology 😍😘😊
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos. Please subscribe and share
@sulemankhanvirologist5015
@sulemankhanvirologist5015 5 лет назад
In sha Allah sir
@mutaheramutahera5824
@mutaheramutahera5824 6 лет назад
Very good explanations..
@madhukarbellary6983
@madhukarbellary6983 6 лет назад
Very good presentation.
@gracekollipara9185
@gracekollipara9185 3 года назад
This is so useful . Hats off for your effort sir. With the help of your video I hope I can give my best in my seminar
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@eaktakandpal6445
@eaktakandpal6445 5 лет назад
Thanks for this valuable information it is very helpful
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad you liked it
@ashishmohankhare6753
@ashishmohankhare6753 6 лет назад
thank u sir ...very nice explaination..keep posting such videos
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+aShish Khare glad to hear that
@mountcarmel7116
@mountcarmel7116 3 года назад
what is the effect of the similarity or difference polarity of the mobile phase and the solute we are computing; does it make a difference. i think i did not hear you saying about it except you said about the polarity of the stationary phase versus the solute.
@sreelakshmiperozhi
@sreelakshmiperozhi 4 года назад
Thank you for such a wonderful session.This lecture helped me a lot............Thank you so much
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@sreelakshmiperozhi
@sreelakshmiperozhi 4 года назад
@@shomusbiologyofficial Sir, I am persuing my graduation in BOTANY right now... and during these lockdown days I attempted to explain the first chapter of CBSE 10th std BIOLOGY (Life Processes ).. @my channel sreelakshmiperozhi Sir please see that and give me your most valuable suggestions........... You are the right person to hear from... Please sir..
@sushantpatil5169
@sushantpatil5169 5 лет назад
You are amazing.... your explanation language is very easy to understand easily thank you so much
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Thank you very much for appreciating my efforts
@khantheelucidator3018
@khantheelucidator3018 3 года назад
I help my friends understand such concepts and Sir Shomu you are the reason why they are getting the concepts.... Thank you very much
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Thank you so much for appreciating my efforts
@chitradhauria9420
@chitradhauria9420 6 лет назад
thank you sir you help us a lot
@logeshwarim2097
@logeshwarim2097 4 года назад
U r simply amazing sir ....
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Thank you so much
@jyoti2298
@jyoti2298 4 года назад
Thank you sir .... your vedios really helping for me ..... thank you sir 🙂❤️❤️
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@raginirawat1915
@raginirawat1915 6 лет назад
awesome sir... please also make video on Thermogravimetry Analysis and Potentiometric titration... i've got my externals on 7th of march 😢
@merriamwebster377
@merriamwebster377 4 года назад
nice explaination sir...
@sultanshashaik8146
@sultanshashaik8146 6 лет назад
thank u sir,,, your videos are super i am watched your every videos ,,, its very useful videos ,,,i am also subscribed your channel
@nijoradeka5294
@nijoradeka5294 5 лет назад
Be a teacher as like you....thanks a lott sir....
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@udayakumaram314
@udayakumaram314 4 года назад
so amazing your guidance and keep going...
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome
@novembern5973
@novembern5973 5 лет назад
very helpful thanks sm
@kavyap4453
@kavyap4453 3 года назад
Thank you for the great way of explanation and the lecture
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@hbbsanyasinaiusaragaradm2574
@hbbsanyasinaiusaragaradm2574 6 лет назад
good explanation Sir
@ramachandransekar3751
@ramachandransekar3751 5 лет назад
Wow... Thank you so much bro... Great job
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Thank you. Glad you liked my lectures
@marjiasultana7658
@marjiasultana7658 6 лет назад
very very and very well explained
@visheshshukla1533
@visheshshukla1533 5 лет назад
You are fabulous.. really u r doing a very good work..love from Himachal
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@lalitabhimewar3021
@lalitabhimewar3021 3 года назад
You are just awesome sir..very nice explanation..the bestest ever👍😍
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
Glad to hear that you're getting benefit from my lectures
@mekhelegaaurjitega3246
@mekhelegaaurjitega3246 6 лет назад
Very nice lecture pls upload whole bsc point
@catarinavagari8965
@catarinavagari8965 3 года назад
This was fantastic; thank you! I really appreciate the work you put into this.
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@catarinavagari8965
@catarinavagari8965 3 года назад
@@shomusbiologyofficial You have no idea how much this is helping me right now during the pandemic when I don't have the same level of access to help. Please keep up the great work!
@habiblucky5259
@habiblucky5259 4 года назад
amazing, you are an extraordinary sir!👌
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Thank you so much for appreciating my efforts
@rishitapatel7543
@rishitapatel7543 2 года назад
Thankyou so much.. today is my examination and your video being soo helpful for me...
@shomusbiologyofficial
@shomusbiologyofficial 2 года назад
Thank you so much for appreciating my efforts
@swostishrestha3624
@swostishrestha3624 5 лет назад
Truely very well explaned 🙏Thank you so much !! Please make video on interferences in electrophoresis 😢🙏
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@luckylahari1348
@luckylahari1348 2 года назад
super and good explanation
@siddheshbachhav7430
@siddheshbachhav7430 3 года назад
Concept of tlc in very simple language 🙏
@anithakumaryab2571
@anithakumaryab2571 6 лет назад
sir ...the class was good...nice lecture....please do more lyk this...
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+NAVANEETH s Kumar thank you
@chinmayeemohapatra2826
@chinmayeemohapatra2826 3 года назад
Very nice explanation sir thank you for making video on this topic 🙏
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome. Glad to hear that you're getting benefit from my lectures
@medicabdullah9789
@medicabdullah9789 2 года назад
You're the man!
@msd8264
@msd8264 5 лет назад
Nice teaching sir. What are the differences between chromatography in biology and chemistry in Csir chemical science exam oriented sir.
@ahmadscience9692
@ahmadscience9692 5 лет назад
very good personشخص رائع
@khadizakhadu4080
@khadizakhadu4080 4 года назад
Amazing i m speachless
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
Thank you so much for appreciating my efforts
@farwanaveed997
@farwanaveed997 5 лет назад
Its amazing sir .... Thank u so much
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
Glad to hear that you are getting benefit from the videos
@shallukhatri4592
@shallukhatri4592 6 лет назад
Pretty helpful.. Thank you.. 😊
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+Shallu Khatri you're welcome
@sitaramahirwar1584
@sitaramahirwar1584 5 лет назад
A lot of help Thank You Sir
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
You're welcome
@vaishalisonvane3965
@vaishalisonvane3965 6 лет назад
amazing... keep it up
@lovepeople4271
@lovepeople4271 6 лет назад
Allah give reward and help in your work
@kalyanlohar476
@kalyanlohar476 Год назад
Very nice informative lecture sir... Excellent.. Thank you 🤗🤗🤗💞
@shomusbiologyofficial
@shomusbiologyofficial Год назад
Thank you so much for appreciating my efforts
@imtamang9800
@imtamang9800 6 лет назад
Very helpful
@shomusbiologyofficial
@shomusbiologyofficial 6 лет назад
+mina tamang you're welcome
@ranjansaikia5125
@ranjansaikia5125 6 лет назад
Sir thank u very much, can u plz elaborate about dark field and phase contrast microscope, plz sir
@payalasreenivas
@payalasreenivas 7 лет назад
good morning sir,your lectures were so inspirational continue. .......
@shomusbiologyofficial
@shomusbiologyofficial 7 лет назад
+Payala Sreenivas thank you.
@ismaelnaruto30
@ismaelnaruto30 6 лет назад
mashAllah brother, keep it up
@akhilsusmith5255
@akhilsusmith5255 5 лет назад
Great job brother Great explanation skills...
@shomusbiologyofficial
@shomusbiologyofficial 5 лет назад
You're welcome. Glad you liked my lectures
@SafdarHussain-cn3fr
@SafdarHussain-cn3fr 3 года назад
thanks for sharing such informative knowledge
@shomusbiologyofficial
@shomusbiologyofficial 3 года назад
You're welcome
@lazarusraju7169
@lazarusraju7169 6 лет назад
is it helpful for qualitative analysis,sir???to determine what components are actually present in the sample?
@nancyjajodia7977
@nancyjajodia7977 4 года назад
Amazing explanation!
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome
@samsungkhana3500
@samsungkhana3500 4 года назад
Thank u sooo much sir...it will help for me
@shomusbiologyofficial
@shomusbiologyofficial 4 года назад
You're welcome
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