Thin Layer Chromatography (TLC)

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We know how to perform extraction, which can separate compounds on the basis of differing solubilities. But what if they have the same solubility? Well there is another technique that utilizes differences in polarity, and it's called chromatography. There are many varieties of chromatography, but the best way to introduce the concept will be to look at thin layer chromatography, or TLC for short. Check it out!
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Опубликовано:

17 май 2024

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Комментарии : 137

@skxjxhxh 4 года назад

You just summarized our 3-hour lesson into 9.5 minutes

@mariadelcarmenarteagasanch8822 3 года назад

exactly, He is the best

@emteemte3985 Год назад

He is my hero chemist. I love orga.che. lab because of him

@masskiller9206 3 года назад

7:52 "It’s usually difficult to predict what will work best, so just try something and see what happens. " professor dave motivates

@drewkrahn 2 года назад

So much easier to understand than our lab manuals. Thank you!

@ramil8029 5 лет назад

Getting ready for the MCAT exam. Your videos are THE BEST on youtube for all of ochem and gen chem material! Thank you so much!

@kevinnguyen1133 4 года назад

Same here!

@minaabdulaal5549 2 года назад

Hope you got into med school :)

@mr.umbrella3979 7 месяцев назад

this is so sweet@@minaabdulaal5549

@meganevans8006 3 года назад

I don't know why o-chem lab manuals have to be mystery novels. Thank you for your videos!

@andym.s.5231 2 года назад

Mystery novels 😭😂😭

@patiencechepkorir5596 2 года назад

I Know right 😂😂

@Alex-gb4vn 3 года назад

DAVE. Keep up the good work. Thank you so much for your contributions. You really explain things in a manner that is so straightforward and easy to follow. I appreciate you so much.

@lalaland774 4 года назад

Since we’re on quarantine my Organic Chem lab professor is using your videos and basing the work he gives around your videos

@danielmeaders4005 4 года назад

This honestly seems like an issue of legality and compensation. The situation is the same at my university (however not for this video), I don't think your school is writing checks to professor Dave... but they are certainly getting their monies.

@XTheSpartanX7 3 года назад

i wish mine did that

@jameshall1300 Год назад

@@danielmeaders4005 it's probably kind of new legal ground to be honest. Before quarantine happened and e-learning became almost the de facto standard in most places, using internet resources so heavily in class was probably very limited. I'm not sure ad revenue really would be fair compensation for that type of usage.

@iuliiamairansaeva9169 2 года назад

I'm happy you've decided to do youtube... Some of us watching you right now will become great chemists in the future, and you'll be one of the main reasons we'll manage

@nikkaagbayani8905 3 года назад

Mahal na po kita Professor Dave. Ikaw po ang pinakadakilang guro na nakakapagpaintindi saakin ng mga bagay na mahirap intindihin.

@sarahholland5980 3 года назад

thank you! prepping for lab quizzes using your videos bc they are so much easier to understand than our manuals

@veronicaaguiniga8496 Месяц назад

Being a visual learner instead of trying to understand material that needs to be read, I feel that this video on TLC should be shared with students that would like a better insight/understanding on this topic. From watching this video I actually learned that the TLC plate itself has hydroxyl groups that is dipole-dipole, allowing it to interact with other polar substances.

@ginamarie7603 3 года назад

great for visual learners, finally a video that makes it easy to understand. thanks.

@senku7996 3 месяца назад

I'm an msc student and you helped me pass my enterance exam and now helping with my final exams in grad school, you're a life saver! :')

@R_samurai779 3 года назад

Thanks a lot man! Our schools told nothing about how this is typically used as an analytic technique

@BIAKANOORWorld 5 лет назад

always satisfied with your explanation prof., a huge fan of you from India

@PG-qn8od 5 лет назад

Jesus, man, your oc videos are helping me sooo much right now, thank you a lot! ❤️

@gabyyoga8385 3 года назад

Incredibly easy to understand thank you so much!

@ParedonesAmarillosNO 4 года назад

What solvent would you use to prepare the samples for cannabidiols. Thank you for your class! Love learning.

@elmirazarei2306 3 года назад

well and a simple-explained tutorial. tnx, prof.!

@bhawanagahlot9540 4 года назад

Explaination which u gave is amazing.. u have professional teaching skill...

@Ambient_Scenes 3 года назад

Thank you for the video! One question: If you have no clue what your sample is made of, how do you know whether to use UV light, or a stain to visualize the dots? Also how do you choose the right dye? Also, what do you do if some components of the mixture only get stained by on stain, while other components only get stained by another stain? Is there a huuuuge amount of different stains the organic chemist has to experiment with or are there just a few that will cover pretty much most chemicals?

@sarahmitchell8344 3 года назад

This has changed my life

@ValentinaLopez-bv6ro 3 года назад

Perfect explanation. Thank you

@pallobbarai4152 2 месяца назад

Thank you, your lecture is amazing, easy to understand, animation is excellent.

@ayasamer7731 3 года назад

At the morning of my exam .. soo helpful a huge thanks to u ❤

@emteemte3985 Год назад

U r unique .i take lab abt tlc anc CC and you make it easy and clear thank you. I wish you good health and long life

@mr_mars_vr 2 года назад

Thank you for this.

@aang7505 4 года назад

thank you so much! I just wanted to ask, do you benefit more from patreon or from RU-vid channel memberships? I really appreciate your help, this explanation was so clear

@ProfessorDaveExplains 4 года назад

Patreon takes a smaller cut so that is much better, thanks so much for your support!

@kikimiki177 Год назад

is the polarity of the samples the only thing that impacts how far each of them will travel, or does their relative mass also have an impact?

@user-kr4ns9vk7j 2 года назад

Hello Doctor I am now working on an experiment, which is to prepare Schiff bases and run by escalation with the condenser for no less than 6 hours, and I want to follow the end of the interaction with the technique TLC (The solvent used for the reaction is ethanol The question is what polar and nonpolar solvents can be used in chromatography and what ratio shows the endpoint of the reaction )

@chickenlover4ever 2 года назад

Amazing video, thank you so much!

@mahmoudothman7910 5 лет назад

Nice new hair-cut Dave 😊

@siddheshdeshpande3034 2 года назад

Avadli ka tula

@Jdoug305 2 года назад

@@siddheshdeshpande3034 yes I like

@siddheshdeshpande3034 2 года назад

@@Jdoug305 mr cheeks from Maharashtra

@Jdoug305 2 года назад

@@siddheshdeshpande3034 aklakam brother 😁

@vintilovwhitefox5365 10 месяцев назад

Thank you dude, it helped me a lot

@lyricmelody4650 3 года назад

This is so helpful! Thank you :)

@vvideo7162 Год назад

Wow sir it's an amazing lecture I have ever seen thanku so much sir ,for clear my doubts 🥰🥰🥰🥰🥰

@ihechisomakagha2568 7 месяцев назад

Just Thank you ❤

@Leen-ih8ql 2 года назад

what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help

@benzjoshwade3462 3 года назад

Hello. This might be a dumb question but why does Silica (SiO2) have hydroxy groups?

@anounymous7988 2 года назад

You're amazing bro god bless you

@elizaahmed2066 2 месяца назад

Sir my compounds are non polar compounds. so which solvent do I use for dissove the compound and what solvents do I use in the mobile phase? sir can you tell me a little bit? I am facing a lot of problems in tlc checking

@sohampowdwal8180 Год назад

in professor dave we trust

@mawadaabdalaal9916 Год назад

شكراً جداً بسيط و مختصر ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️

@mohinikulkarni4129 2 года назад

I loved watching this TLC 👍

@sagagayathri9570 4 года назад

I had a doubt.... how to know what component has been seperated...and also what is the use of rf value

@asmaaaboalmagd792 Месяц назад

Thanks professor

@cesca3994 4 года назад

well explained! Thank uuuuuuu.

@sweetoldsoul4454 2 года назад

Thank you 🙏

@Thaumius 5 лет назад

Will you be doing HPLC/GC tutorials?

@ProfessorDaveExplains 5 лет назад

i hadn't planned on it, but i'll put it on the list! it is a big one.

@moonlillac Год назад

thankyou, it help me so much.

@chemistryforsociety3448 2 месяца назад

Excellent...... thanks a lot

@LOADEDUPTV 5 лет назад

Hello Dave, please talk about Keto Enol Tautomerism

@ProfessorDaveExplains 5 лет назад

check my tutorial on michael addition

@luftatmer 4 года назад

man that was a great video!

@YouMockMe 4 года назад

I came back to this a few times haha... great vid

@mustakim2144 3 года назад

Thank you!

@carolynenglish3068 3 года назад

Brilliant!

@chorgulio 4 года назад

So what is the typical solvent used for TLC? would that depend of the sample/compound to be analyzed?

@ProfessorDaveExplains 4 года назад

in the lab i would typically use a mixture of hexanes and ethyl acetate. it's kind of personal preference to an extent.

@mariamgad284 5 лет назад

very excellent 💙💙

@chemistry7554 4 года назад

Good personality with hair cut,by the way vedio is help full............

@pintukumargupta8415 10 месяцев назад

Nice easy to understand thaks

@tanzimnishad3892 Год назад

Sir How can I separate a mixture with 37 compounds in it using column chromatography

@specialparadise 5 лет назад

Wow what a haircut Professor! You look good either way lucky you.

@DapaChrons 5 лет назад

New haircut! looking good either way

@kennyyong7694 3 года назад

Excuse me, do you know how to prepare silica gel to absorb moisture or silica gel to act as the stationary phase in thin layer chromatography. I need to make my own TLC plate

@ProfessorDaveExplains 3 года назад

Hmm no I've never made plates before. But check the tutorial that follows this one on column chromatography, it may help a little.

@tarahclue6737 2 года назад

Thank you

@esmeralda804 8 месяцев назад

ME ENCANTAAAAA❣

@pharmacist9193 2 месяца назад

Thank u❤❤❤

@karenopt 4 года назад

If the solvent is polar, then the polar molecules will have a smaller Rf and non polar molecules would have a larger Rf? Or is it the other way around?

@ProfessorDaveExplains 4 года назад

yes you have it right!

@saiteja6742 4 года назад

Sir why do we use organic solvent(toulene) as mobile phase in thin layer chromatography ?? Pls reply for this sir

@ProfessorDaveExplains 4 года назад

what else would we use?

@yasigerman731 Год назад

thank you thank you thank you

@137bob3d 4 года назад

superb presentation. and good of you to cite how TLC is a stepping stone to column chroma' ... and to work out the solvent(s) x:y ratios w/ TLC for upper half separation. eager to see the col. chr. vid's on any time-correlation in top to bottom flows vs TLC. and how you go about collecting the eluent with the separated product and nothing else. also will be listening for info on the details of how one chooses a silica mesh size. and possible re-usability of silica for those of us who know how costly that stuff is. and lastly how to choose column size vs number of grams of material to be separated.

@roseycheekies51 2 года назад

i love you dave

@natehcsingh8634 3 года назад

Came in jee mains 2021 february attempt . Thanks Jesus↗↗

@superhessu3 2 года назад

4:56 just marking for my upcoming university course

@ayaalhashimi3100 2 года назад

Please what mean tlc its same column ?

@HeyImLucious 5 лет назад

If only this video came out a few years ago... I wouldn't have looked like such a fool in my research lab haha

@prateekkulkarni2639 Месяц назад

A day before jee mains 2024

@Ambient_Scenes 3 года назад

Why do you need a capillary tube to place the solution mixture on the plate? Can't you just use a pipette or dropper or something?

@ProfessorDaveExplains 3 года назад

the capillary tube is much better for applying the solution because you press it against the plate and it is adsorbed, a dropper would be terribly imprecise

@Ambient_Scenes 3 года назад

@@ProfessorDaveExplains Thanks for clarifying.