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This is the easiest way to make plant tissue culture media! 

Plant Cell Technology
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Want to get started in plant tissue culture but not sure how to make plant tissue culture media? Well, we've got something special for you: our very own lab director, Francisco, is spilling the beans on "The Easiest Way to Make Plant Tissue Culture Media."
This video is perfect for anyone who's been curious about tissue culture but didn't know where to start.
Francisco has figured out a method so simple you'll be wondering why you haven't tried it sooner. He walks you through every step with such clarity that even absolute beginners will feel like pros by the end of the video. It's all about breaking it down to the very basics of the process and showing you that, yes, you can do this at home!
And if you're thinking, "This is awesome, but I want to learn even more," then you're in luck! We're hosting a master class that's literally designed to turn you into a tissue culture PRO in just two days. Check it out and sign up here: plantcelltechnology.com/master-classes/
Time Stamps:
• 0:01 Intro
• 0:10 Materials and Equipment
• 1:00 How to Make Tissue Culture Media
• 4:30 Calibrating the pH
• 6:43 Pouring media into vessels
• 7:02 Adding Agar to the media
• 9:05 How to autoclave the media
• 10:00 Adding plants to the tissue culture media
• 10:50 Outro
💡 Special Offer: For those kickstarting their tissue culture journey, use Francisco's link to save 10% off your first purchase: www.plantcelltechnology.com/?...
Don't miss out on all the fun and learning. Follow us on our social media channels for more tips, tricks, and plant science magic:
🌿 INSTAGRAM: / plantcelltech
🌿 FACEBOOK: / plantcelltechnology
🌿 LINKEDIN: / plant-cell-technology-...
🌿 TIKTOK: / plantcelltech
🌿 TWITTER: / plantcellnews
🛒 Products We Used in the Video:
- Supreme-Grade Agar: bit.ly/3T4nZy3
- MS Media w/ Vitamins: bit.ly/3YPAaAp
- PPM™: bit.ly/3ZLhXES
- Gellan Gum: bit.ly/421HWto
- IBA Solution: bit.ly/3ZysQKA
- NAA Solution: bit.ly/3LsMP9p
- BAP Solution: www.plantcelltechnology.com/b...
- TDZ Solution: www.plantcelltechnology.com/t...
- Kinetin Solution: www.plantcelltechnology.com/k...
- Square Media Vessels: bit.ly/3J7yigg
- Round Snap-Lock Containers: www.plantcelltechnology.com/r...
- Biocouplers™: bit.ly/3J3aD0r
- Biocoupler™ Glass Set: bit.ly/3mHm6v3
- Biocoupler™ Bundle: bit.ly/3Jui3eR
- Laminar Flow Hood: www.plantcelltechnology.com/2...
#planttissueculture #plantcelltechnology #tissueculture #micropropagation #plantscience #tissuecultureplants #tissueculturemedia

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6 июл 2024

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Комментарии : 25   
@steeeebs7286
@steeeebs7286 3 месяца назад
Francisco, where do you get those containers? The ones we got from Karat melt in our autoclave.
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
They're the Round Snap-Lock Containers from our PCT Store, you can find them here: www.plantcelltechnology.com/round-snap-lock-containers/
@chuck45790
@chuck45790 3 месяца назад
Hi. I have a quick question. Can i take a small tissue culture plant with roots puchhased from ebay and put it a biocoupler or would there be issues
@Parkfolia
@Parkfolia 3 месяца назад
Genius idea!! 🤯 How important is it to make media and sterilize it right away? Would it affect the media at all to leave that jug in the fridge for a day or two (and hope nobody tries to drink it 😅)
@daytrails
@daytrails 2 месяца назад
Giving this method a try now. Thank you for sharing. Any concern with using RO water? It comes out of my filter right around the target pH. EDIT - I re-watched the video, you mention reverse osmosis is OK around 0:55
@PlantCellTechnology
@PlantCellTechnology 2 месяца назад
That's great!
@chugginbeers
@chugginbeers 3 дня назад
4.5 grams of ms medium? Murishige and skoog with vitamins?
@PlantCellTechnology
@PlantCellTechnology 2 дня назад
yes, that's right!
@assassins_garden_nm
@assassins_garden_nm 3 месяца назад
If the Agar doesn't gell up what do I need to do? Add more agar?
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
How much agar do you use in 1 L of media?
@AquaMayne
@AquaMayne 3 месяца назад
Does plant cell technology’s ms media not have sucrose? Oh no my plants I just TC’d might be In Trouble 😢
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Uh oh! Yes, our media doesn't contain sucrose. It's mentioned on the website and will also be written in the manual. "MURASHIGE & SKOOG MEDIUM (W/ VITAMINS & CALCIUM CHLORIDE, AGAR)"
@daytrails
@daytrails 2 месяца назад
You are not alone! I just made this same mistake with my last batch, too.
@AquaMayne
@AquaMayne 3 месяца назад
Will my plants die if I did not add sugar to the PCT media?
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Yes, sugar is the source of carbon for plants. So, you need to add it to the tissue culture media.
@AquaMayne
@AquaMayne 3 месяца назад
Thanks, Just re-did all my cultures @@PlantCellTechnology
@Kay42oo
@Kay42oo 3 месяца назад
why aren't you not adding any pgrs?
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
PGRs are added at the multiplication and rooting phase of plants. Generaly, the initiatin media doesn't involve PGR. But, it totally depends on the protocol you follow for your plant.
@gardeningonly
@gardeningonly 3 месяца назад
Why my media is solid?
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Solid media results from adding agar or gellan gum (a solidifying agent) to the media.
@gardeningonly
@gardeningonly 3 месяца назад
@@PlantCellTechnology thank you
@sallymark8778
@sallymark8778 Месяц назад
you are to far from the mic
@chrisna5668
@chrisna5668 3 месяца назад
So counting 20 drops is easier than the flick of a thumb? No thanks I'll continue to use my metered pipette before I'm counting drops.
@KyLinda
@KyLinda 3 месяца назад
you know? why say anything at all?
@JustMe-fl1db
@JustMe-fl1db 3 месяца назад
It's easier to buy pre-made media... Perspective, tools-on-hand, and budget are all factors that determine the meaning of "easy."
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