Making Tissue Culture Media for 100 Deli Containers!

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Watch our lab director, Francisco, make media and sterilize 100 Deli Containers to transfer some plants into new media for our upcoming Tissue Culture Master Class: Houseplants this January: www.plantcelltechnology.com/m...
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We are Plant Cell Technology, a leading plant tissue culture industry organization since 1995. We develop, produce, and market innovative solutions for global plant tissue culture operations and hobbyists. Our well-researched, certified, and trusted products keep your cultures healthy and contaminate-free.
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Опубликовано:

21 дек 2022

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Комментарии : 37

@dr.greenthumbs345 Год назад

The tip about using the slow cooker liner to autoclave the deli containers is priceless. Thanks! The larger "Oven Bags" are also available if anyone is interested. Made from the same nylon blend as the slow cooker liners. They are 23" x 19" and heat resistant to 400°F / 204°C.

@jaybien1 Месяц назад

YES

@AndresAQChilo 8 месяцев назад

Muchas gracias. Excelente video!

@PlantCellTechnology 8 месяцев назад

Thanks! Happy to know that you enjoyed the video!

@BB-dd1qk Месяц назад

Ok here’s a hack go to restraint supply buy ketchup cups and lids They are gamma sterilized, so if you just open them in front of your Flow Hood, important to them and put your lids on, you’ll be fine

@hxx117 Год назад

What is the Plant Cell Technology catalog equivalent of those extra vitamins 16:46 you applied and what is the dosage for both houseplants and carnivores? Thank you for so much knowledge, Francisco!

@LilHer08 Год назад

@Plant Cell Technology - you added an extra ml of BAP to the center beaker. Time stamp 16:34. The start of you adding BAP was 16:14. Just thought I would let you know as I am not sure what the outcome could be.

@PlantCellTechnology Год назад

Great catch. Thanks for sharing.

@Astrid-oq3kk Год назад

Hi, I've noticed you calibrate the PH before adding the gelling agent, why is that? Doesn't gellan gum risk impacting the PH of the medium?

@PlantCellTechnology Год назад

Hi Astrid, a good quality gelling agent does not change the pH of your media. The reason I calibrate the pH before adding the gelling agent is to keep the probe clean. I also added the gelling agent to the bottles.

@Astrid-oq3kk Год назад

@@PlantCellTechnology OK thanks :) I'm stuck with a big bag of poor quality agar at the moment (it has sugar in it, so I deduct the sugar from my recipe) so I think I'll keep measuring after adding it at the moment

@alfred282 3 месяца назад

Great video! Thanks for sharing.. just wondering, how long do you autoclave the container and the 1 litre media?

@PlantCellTechnology 3 месяца назад

You need to autoclave the media at 15lbs (121°C) for 15 minutes.

@alfred282 2 месяца назад

@@PlantCellTechnology thanks for the reply!

@PlantCellTechnology 2 месяца назад

@@alfred282 Happy to help!

@nakamurajinusuke Год назад

what grow light do you use for tissue culture?

@PlantCellTechnology 4 месяца назад

We generally use LED lights for the tissue culture plants.

@charliemagoo7943 9 месяцев назад

in this video you seal the lids before cooling . others you seal the lids???

@ShubhamKumar-fh5hd Год назад

Can I use UV light for sterilization?

@PlantCellTechnology Год назад

No. For effective tissue culture media and tool sterilization, the autoclave is essential.

@adedoyinadetunji2078 10 месяцев назад

what is the size of your pressure cooker?

@PlantCellTechnology 10 месяцев назад

It comes in different volumes. so, depending on how much media you need to sterilize you can choose either a 5L, 10L, or 20L pressure cooker for your use.

@alexiszisakos295 7 месяцев назад

I just started with tissue culture and was wondering if your hormones are heat stable or simply do not degrade with the temperatures in the pressure cooker? Sadly, i'm not able to buy the ingredients from PCT since the shipping is more than a hundred bucks, so i hope this counts for the hormones of other companies too.

@PlantCellTechnology 7 месяцев назад

Some plant hormones are autoclavable and some are not. Which hormones do you want to know about? Also, we have distributors worldwide who can help you save on the shipping cost. If you can let us know where you are located, we can connect you to the closest distributor to help you with your tissue culture requirements.

@alexiszisakos295 6 месяцев назад

@@PlantCellTechnology Thank you for your answer! I'm currently using NAA, IAA, IBA, Kinetin and BAP. I'm located in south-western Germany and would love to use the products of PCT! Also, it would be great to know with which amounts i need to compensate a potential loss of phytohormones which are partly degrading under 120°C.

@PlantCellTechnology 6 месяцев назад

Hi Alex, Here are the details of our German distributor: They will help you with all of your tissue culture requirements. Also, we couldn't get what you wanted to say with this "Also, it would be great to know with which amounts I need to compensate a potential loss of phytohormones which are partly degrading under 120°C." Can you rephrase the part so we can help you better? Thanks! @@alexiszisakos295

@alexiszisakos295 6 месяцев назад

@@PlantCellTechnology Sure. So the datasheet of my hormones says that all of the hormones i'm using are autoclavable (marked with a CA) while some are marked with CA/F. So the hormones are autoclavable but there is some potential degradation during autoclaving which can be compensated by adding a little more. So the question is how much is basically degrading... Is it 1/4 or half of it? (I know i can simply add them by sterile filtration 😄)

@nejc1 6 месяцев назад

@@PlantCellTechnology Can you share details of the German distributor, I'm interested in some of your products too.

@tzi0m 4 месяца назад

What is the size of your containers?

@PlantCellTechnology 4 месяца назад

These are 12 oz containers. We sell the products we use on our website!

@ShubhamKumar-fh5hd Год назад

Is there any alternative of tween 20 ?

@PlantCellTechnology Год назад

You can use Triton-X-100. Get more insight on this page: www.researchgate.net/post/What_can_be_used_as_a_cheaper_alternative_of_tween_20_especially_for_plants_having_more_dense_trichomes#:~:text=We%20use%2070%25%20ethanol%20for,Good%20luck.

@BB-dd1qk Месяц назад

Few drops of peroxide per liter