Raph- darker skin, bolt plastron scar, green eyes, and simply the attitude face he has. Leo- more like a bluish green skin, blue eyes, smoother head, and the calm look. Donnie- Oval head, gap tooth, paler skin, taller thinner look, and that grin. Mikey- Freckles, Lime green skin, baby blue eyes, flatter head, slightly more pudge, that big goofy grin and silly personality.
That "wake up leo" scene is from frozen isn't it? Also, good story on the one where they all think Splinter doesn't recognize them. But why are they already calling him Sensei? Wouldn't they have been calling him "Dad" or something like that at such a young age?
Leo has cyan eyes, Raph has lime green, Donnie has red/urban and mikey has baby blue. There eyes are how I tell them apart, also Raph has a chipped shell shaped like a lighting bolt. Mikey and Donnie are the lightest skin tones. Raph is the darkest, and leo is second to him.