The separation of different polar analytes is dictated by the choice of HILIC stationary phase. The method development procedure we presented in the video is an universal approach for any regular silica-based column. As this type of column is not stable under high pH, we do not recommend to use basic buffer for the mobile phases. Again, selecting a suitable HILIC stationary phase and work on the pH range from 2-7 is the key for method development.
