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What Are: Knockout Mice? 

Cyagen
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From the Creation of the First Knockout Mouse Scientists have come very far; with CRISPR technologies being the main method used by genetic knockout scientists today, the traditional esc based technology explained in this video is something of the past, mainly due to the length of time these projects take to complete (8-12 months).
Please note that any percentages mentioned in this video should be taken with a grain of salt as they are not exact values. For example, the targeting efficiency of vectors vary greatly not only from loci to loci, but also from vectors to vectors. 10% is just a rough average targeting efficiency in our hands.
More information about our knockout mice could be found at:
www.cyagen.com/us/en/service/...
Cyagen is the worlds leading provider of genetically modified mice and rats. We have produced over 50,000 projects to date. We also specialize in embryonic stem cells and the special media used in this video for research purposes.
You can find all our products at www.cyagen.com
Website: www.cyagen.com/us/en/
Twitter: cyagenbio?lang=en
RU-vid: / @cyagen
Linked-in: / cyagen-biosciences
Facebook: / cyagenbiosciences
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17 дек 2020

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Комментарии : 26   
@Cyagen
@Cyagen 11 месяцев назад
More information about our knockout mice could be found at: www.cyagen.com/us/en/service/crispr-based-genome-editing-knockout-mice.html
@rhyslormand703
@rhyslormand703 8 месяцев назад
Explained 100x better than my professor! Awesome video.
@Cyagen
@Cyagen 8 месяцев назад
Thanks, we are happy it helped better explain knockout mice for you!☺
@timeaboroczky3364
@timeaboroczky3364 3 года назад
Thank you ! very helpful and informative
@izzyp1162
@izzyp1162 2 года назад
quick and easy explanation, thanks.
@Senara_99
@Senara_99 2 года назад
Thank you very much! ❤️
@zhansayabauyrzhanova2492
@zhansayabauyrzhanova2492 2 года назад
Perfect explanation
@michael2539
@michael2539 2 года назад
Very helpful. Thanks
@KevinT
@KevinT 3 года назад
Hi Cyagen I have a quick question: how are we putting an antibiotic resistance gene into a eukaryotic cell? The stem cells from the blastocyst are from a eukaryotic organism.. so I'd assume that antibiotics wouldn't be able to kill these cells anyways? Please let me know if I'm misunderstanding something. Thanks!!
@jumanajamal8945
@jumanajamal8945 Год назад
I was actually wondering the same thing, why do we add neomycin, if eukaryotic cells don’t get affected by antibiotics.
@zhansayabauyrzhanova2492
@zhansayabauyrzhanova2492 2 года назад
Thank you!
@divinaryf3703
@divinaryf3703 3 года назад
thank you!
@yonetmen
@yonetmen 3 года назад
Is there anyone here who can explain to me why do we always obtain heterozygous cells after homologous recombination?
@ashwinimurthy8733
@ashwinimurthy8733 3 года назад
Bcos AA *aa =Aa crossing over
@richashrivastava3981
@richashrivastava3981 7 месяцев назад
If we do PCR of all ESC, how are we left with cells to be implanted in the new mice blastocyst?
@Cyagen
@Cyagen 7 месяцев назад
Typically, one or more subcultures of cells can be made for analysis and selection of the correctly modified cells.
@richashrivastava3981
@richashrivastava3981 6 месяцев назад
Thanks@@Cyagen
@shri_harshabhat4069
@shri_harshabhat4069 11 месяцев назад
Pls send notes of this
@Cyagen
@Cyagen 11 месяцев назад
Thanks for your interest in our knockout mice. More information could be found at: www.cyagen.com/us/en/service/crispr-based-genome-editing-knockout-mice.html
@sanchitapaul1223
@sanchitapaul1223 3 года назад
Thats helpful.
@ive1111
@ive1111 Год назад
Just wondering, are there technologies or drugs that can knock out Genes by simply giving oral drugs while being pregnant?
@Name-zd5fq
@Name-zd5fq 2 года назад
Nobody: Pfizer: "Let's knock out humanity!" :D
@lucasjones3338
@lucasjones3338 2 года назад
Yeah, after I got the vaccine I stopped existing.
@Name-zd5fq
@Name-zd5fq 2 года назад
@@lucasjones3338 You will never have children.
@KlearChristal
@KlearChristal Год назад
@@Name-zd5fq pretty sure thousands of vaccinated people have already had children lol
@Name-zd5fq
@Name-zd5fq Год назад
@@KlearChristal LOL...let's see how long you'll laugh. :D
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