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What Is Sanger Sequencing? 

Promega Corporation
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Learn about a workhorse technique of lab research since the late 1970's.
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24 авг 2020

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Комментарии : 24   
@promega
@promega 2 года назад
See how the ProDye™ Sanger Sequencing System is used on the Spectrum Compact CE System in a proof-of-concept workflow for environmental microbial monitoring programs that require species-level identification: bit.ly/3Pjvhwm 👉Helpful Links Subscribe to Promega on RU-vid: bit.ly/2YtQhtS More resources on our website: bit.ly/3BFhFna Link to product page: bit.ly/3av8PMX
@hifza2107
@hifza2107 20 дней назад
What is Sanger sequencing? A conventional method used since 1977, is still used today In this we denature the DNA, by increasing the temperature. Cool down to cause primers to anneal. DNA polymerase then extend the DNA fragments until a fluorescently labeled dideoxyribonucleotide is reached. The chain is terminated. The colour of the florescent indicates the base at which the chain is terminated. When the DNA fragments are separated by size, we can determine the sequence of terminal nucleotides and determine the sequence of DNA/genome
@leenahmed147
@leenahmed147 Год назад
Spectacular explanation! It’s amazing how you simplified it thank you
@AliShaikh_744
@AliShaikh_744 5 месяцев назад
Very concise yet gets the concept across in an excellent manner which is very easy to understand! Thank you!
@beckseamons
@beckseamons 8 месяцев назад
Wow. Amazing explanation, it really helped me understand how genius this method is.
@ZARIASALIM
@ZARIASALIM 8 месяцев назад
Great explanation
@greennamm1907
@greennamm1907 5 месяцев назад
Thank you! You're a lifesaver!
@lojainashour
@lojainashour Год назад
Great thanx
@egegezer8220
@egegezer8220 21 день назад
Finally a good video
@user-nf2in4fs5u
@user-nf2in4fs5u 3 месяца назад
Great video! One thing--I think it's misleading to say the ddNTP is "randomly" inserted. It's inserted according to Watson-Crick rules.
@poldi7
@poldi7 3 месяца назад
He means that, by chance, a ddNTP is inserted instead of a normal dNTP, which would not stop elongation.
@user-nf2in4fs5u
@user-nf2in4fs5u 3 месяца назад
Yep, I get that! Just pointing out that his statement could be misinterpreted. @@poldi7
@poldi7
@poldi7 3 месяца назад
@@user-nf2in4fs5u Yeah, that could be true. You‘re right
@rabia4009
@rabia4009 3 месяца назад
I don't understand how we get to know about the sequence and why we are terminating it with ddNTPs?? Can someone help me with this?
@barbarosxeczaci7410
@barbarosxeczaci7410 2 месяца назад
When we terminate the chain with ddNTP’s we understand that in this spesific place there is spesific nucleotide that we added in the tube and after that you repeat the action and figure out all of different spesific places too. I hope i could explain you
@Baseds__Backup_Account_3
@Baseds__Backup_Account_3 2 месяца назад
It's between anger and sadness actually, lol.
@nandagopal-bj8zh
@nandagopal-bj8zh Год назад
wow
@zemaxe13
@zemaxe13 8 месяцев назад
@0:48 ... is missing a hydroxyl group which allows the next nucleotide to bind - you mean it does not allow the next nucleotide to bind, thus ending in termination...
@promega
@promega 8 месяцев назад
Hi there, thanks for your comment. The dideoxynucleotide is what terminates the chain.
@CoalCrafteris
@CoalCrafteris 6 месяцев назад
Hydroxyl group allows the next nucleotide to bind, thus ddNTP (which is missing one) terminates the sequence, he explained it fine.
@aditisoumendra1150
@aditisoumendra1150 3 месяца назад
Yeah, basically we need a hydroxyl group at 3' carbon atom in a pentose, for phosphodiester bond to be formed and for DNA sequencing to happen, right.. so ddNTP lacks that 3' OH group as well. So no further bond formation, and no more sequencing.
@fay4038
@fay4038 Год назад
📚🇧🇷🙏
@aa6678
@aa6678 2 месяца назад
Huh ?
@James-ke5sx
@James-ke5sx День назад
It still doesn't explain exactly how it's done.
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