Learn about a workhorse technique of lab research since the late 1970's. If you like these videos, check out our Student Resource Center for more helpful articles and tools: www.promega.com/MoreStudentRes...
See how the ProDye™ Sanger Sequencing System is used on the Spectrum Compact CE System in a proof-of-concept workflow for environmental microbial monitoring programs that require species-level identification: bit.ly/3Pjvhwm 👉Helpful Links Subscribe to Promega on RU-vid: bit.ly/2YtQhtS More resources on our website: bit.ly/3BFhFna Link to product page: bit.ly/3av8PMX
What is Sanger sequencing? A conventional method used since 1977, is still used today In this we denature the DNA, by increasing the temperature. Cool down to cause primers to anneal. DNA polymerase then extend the DNA fragments until a fluorescently labeled dideoxyribonucleotide is reached. The chain is terminated. The colour of the florescent indicates the base at which the chain is terminated. When the DNA fragments are separated by size, we can determine the sequence of terminal nucleotides and determine the sequence of DNA/genome
When we terminate the chain with ddNTP’s we understand that in this spesific place there is spesific nucleotide that we added in the tube and after that you repeat the action and figure out all of different spesific places too. I hope i could explain you
@0:48 ... is missing a hydroxyl group which allows the next nucleotide to bind - you mean it does not allow the next nucleotide to bind, thus ending in termination...
Yeah, basically we need a hydroxyl group at 3' carbon atom in a pentose, for phosphodiester bond to be formed and for DNA sequencing to happen, right.. so ddNTP lacks that 3' OH group as well. So no further bond formation, and no more sequencing.
