Widefield and Confocal Fluorescence Microscopy 

I am doing PhD now too!

oooh

Nothing like taking a thick stack only to find a massive black square on your embryo when you're ready to shoot your overview. Moral of the story, take your overviews first.

Or may be the case where the wavelength, Which emits , are not belong to visible light

Hmm, I always thought the term applied to any electron moving to a lower energy shell. Does it not? Fluorescence is just a subset of this phenomenon.

@@ProfessorDaveExplains Actually you are correct. Relaxation is only electron movements, from higher energy state to lower ones; either it is non-radiative or radiative. But term of relaxation is mostly understood as vibrational relaxation, which is non-radiative. Some researchers may call the radiative relaxation as transition, although both are the same. For Master student or higher, your term is OK (hopefully they can distinguish), for Bachelor this may distract if you do not explain completely.

Nvm, just found one lol

Yep just that one for now but I’m planning a proper psychology series!

@@ProfessorDaveExplains Thank you, hoping to be a therapist in the future.

@Matt Parker HEY MATT!!! You are a complete and utter ignorant FOOL!! Are you talking about luciferase and luciferin? Yeah. CHEM ICK ALS!!! EVERY THING is a chemical bud!!! You are BREATHING chemicals right now! And you drink a toxic chemical every single day! Ever hear of dihydrogen monoxide?? What about the deadly chemical Pentahydroxyhexanal??? That is inFUSED in you right now! Luciferase is an enzyme. Enzymes are PROTEINS!!! And luciferin is a compound that has fluorescent properties. These are used in the DEVELOPMENT of many biologically active substances, including VACCINES! An assay method was developed using the reaction of this enzyme and its substrate to help determine the efficacy of biomolecules. They are not PART of the COVID-19 vaccines. JUST LIKE human tissue is NOT in the vaccines. Cell lines derived from aborted fetuses from 50 years ago (amazing that these cell lines are still with us), originally developed to help in CANCER research, are also used to develop various vaccines including the mRNA vaccines of Moderna and Pfizer Biontech. The cell lines are use to proof the efficacy of immune responses in human cells. Pull your dumbass out the conspiracy crap and actually learn some REAL SCIENCE!!! Dave didn't answer you because your piddly ass is not worth his time.

@@rickkwitkoski1976 hey Rick, take a break buddy

It absolutely is not impossible. We can sequence genomes. We know the organismal identity of any genetic sequence we are working with. This is trivial.

@@ProfessorDaveExplains thanks for taking the time to respond to my question, i do appreciate it, i thought the question of identifying viruses might relate to the above topic, similar to the use of luciferase to assay transcriptional activity of regulated activation sequences of DNA - pubmed.ncbi.nlm.nih.gov/2698191/ (as in the use of the property of fluorescence to give us information on things). i could ask you about 20 questions and i wouldn't want a focus on a trivial topic but i'll ask is there a method to cause bioluminescence of viruses to identify viruses or can fluorescence microscopy identify viruses? what's the control to distinguish which of the rna is cellular or viral? again, thanks very much for your response

No problem, happy to help, though of course my utility is limited to very general questions such as your first one. I don't know much about the specifics of laboratory techniques as I'm not a microbiologist. Someone else wrote these microscopy scripts.

@@ProfessorDaveExplains Gentleman, thanks very much for your time and patience.

@@ProfessorDaveExplains professor dave, you should make a video on how race realism conspiracy theories are pseudoscientific. thanks for reading.

You will be hard pressed avoiding it 😉

@@DrBovdin i really cant.....it is part of the curriculum. Unless i had chosen the teaching curriculum. Bacharelado 👈

@@mariocesarsousa that’s what I meant. You can’t avoid an integral part of one of the most powerful and most commonly used analysis tools we have in the field.

Look forward to it. It's one of the coolest things I ever encountered in biophysical chemistry!