Workshop 1: FRET application in plant biology 

In many biological research projects, a high spatial and temporal resolution for the observation of in vivo protein interaction is needed, e.g. in order to follow changes of interactions and complex formation over time. In vivo Fluorescence or Förster resonance energy transfer (FRET) measurement allows detailed analysis of interacting molecules in their natural environment at a subcellular level. Especially, FRET-FLIM (Fluorescence lifetime imaging microscopy) measurements provide an extremely powerful and reliable tool meeting the demands for investigating in vivo protein interaction also quantitatively and with high precision. During the workshop, we will introduce in detail how to practically perform in vivo FRET measurements in living plant tissue and discuss potential pitfalls and points of consideration.
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Presenter:
Stefanie Weidtkamp-Peters, Head of Center for Advanced Imaging (CAi) at Heinrich Heine- University Düsseldorf (Germany)
Yvone Stahl, Group leader at the Institute for Developmental Genetics, Heinrich Heine University, Düsseldorf (Germany)
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This video is recorded on 25.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences takes place on November 23. to 27. 2020 via Zoom.
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