Hi, I'm Adwoa, a Master of Public Health graduate and a Biotechnologist. I enjoy learning in a relaxed and animated fashion, so I set out to share my learning style via areas and techniques I use in medical research.
Adwoa Biotech covers techniques you're likely to use in a medical research laboratory.
Are you... 📌 Analysing Western Blot Data? See ⚖️ HOW TO QUANTIFY WESTERN BLOT BANDS using ImageJ | Area Under The Peak Method
📌Performing Drug screening Assays? See 💊 DRUG DISCOVERY AND DEVELOPMENT via High Throughput Screening // UPDATED
📌 Doing CRISPR CAS9 Experiment?: See 🖥️ BIOINFORMATICS OF CRISPR CAS9 | Designing guide RNAs (gRNAs) for Genome Editing
📌Analysing Data?: See 🛑 CONCEPTS IN DATA ANALYSIS: MEAN, STANDARD DEVIATION, FOLD CHANGE & GRAPHING of data in excel
I hope you enjoy learning from these videos as much as I enjoy making them :)
Please consider subscribing if you find the content helpful.
References to deepen your knowledge : Books "Principles of Instrumental Analysis" by Douglas A. Skoog, F. James Holler, Stanley R. Crouch This book provides comprehensive coverage of analytical techniques, including the use of internal standards in various instrumental methods. ISBN: 978-0495012016 "Quantitative Chemical Analysis" by Daniel C. Harris This text covers the principles and practices of quantitative analysis, including the application of internal standards in different analytical procedures. ISBN: 978-1464135385 "Handbook of Analytical Techniques" edited by Helmut Günzler and Alex Williams This handbook includes detailed discussions on various analytical techniques and the use of internal standards to improve data quality. ISBN: 978-3527296539 Articles and Papers "Use of Internal Standards in Quantitative Analytical Chemistry" by Alan G. Marshall and Bruce S. Meyer Journal of Chemical Education, 1977, Vol. 54, No. 10, pp. 640-643. This article provides an overview of the principles and applications of internal standards in quantitative analysis. DOI: 10.1021/ed054p640 "The Role of Internal Standards in Quantitative Analysis by Mass Spectrometry" by Chris B. Stack, David Muddiman Analytical Chemistry, 2011, Vol. 83, No. 12, pp. 4649-4660. Discusses the use of internal standards in mass spectrometry to enhance quantitative accuracy. DOI: 10.1021/ac200160w "Application of Internal Standards in Chromatographic Analysis" by James W. Taylor Journal of Chromatographic Science, 2002, Vol. 40, No. 6, pp. 292-299. Explores the use of internal standards in chromatographic methods to improve quantification. DOI: 10.1093/chromsci/40.6.292 Online Resources National Institute of Standards and Technology (NIST) - Analytical Chemistry Division The NIST website provides guidelines and resources related to analytical methods, including the use of internal standards. NIST Analytical Chemistry Division International Union of Pure and Applied Chemistry (IUPAC) - Compendium of Chemical Terminology (Gold Book) The IUPAC Gold Book offers definitions and explanations of terms used in analytical chemistry, including internal standards. IUPAC Gold Book Practical Guides "Quantitative Analysis Using Internal Standards in HPLC" by Jürgen Hochstrasser A practical guide on the application of internal standards in high-performance liquid chromatography (HPLC). Available online as a technical note from various chromatography companies.
Oh sorry to hear that it wasn’t clear for you, but thanks for the feedback. Will keep it in mind for future tutorials. In the meantime you can let me know what was not clear.
LOL! I appreciate the feedback. I will try to make future videos clearer. Was there any question you had in particular that I could try to answer for you?
I did DNA extraction by method of Maxi prep for the first time. I didn’t even understand the others steps. I’m willing to learn more methods and other steps.
It takes time to digest all the information but repeat it several times and it'll be locked in. The Maxiprep allows isolation of larger amounts of DNA. What was your sample source for the extraction?
Greetings from America. I love your teaching approach Adwoa. Your channel is one of my favorites! Can you create a video similar to this showing how internal standards are used for a calibration curve. From what I understand ratios are key for the internal standard approach. Any information on this would be much appreciated!
Hello Doctor! I was diagnosed with HTLV-1, I have hyperthyroidism, osteoarthritis in my spine and osteoarthritis in my knees (I had surgery on my spine and a knee replacement). Do these bone problems and hyperthyroidism come from HTLV-1? I am 64 years old and female! A hug from Brazil!
Hello! Apologies, I'm not a doctor. I am not aware of these conditions being caused by HTLV-1, so please consult your doctor. Here's a link to a video discussing the causes of hyperthyroidism: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-hxzJxCONxqk.html I pray you find the underlying cause, so that you can find curative treatments. All the best!
I want to ask a question. My English skill is not enough to understand . It could be silly question sorry . I am trying to learn statistics . I would love to do statistics of my researches my own . I want to do a research about "Mortality and complications after percutaneous endoscopic gastrostomy)PEG): a retrospective one-centered study" My universe is 908 PEG . After excluding criterias I dont know how much will stay but probably around 600 cases I want them all. How can I calculate for that retrospective research sample size. 1-After PEG minör complication rate : %18-38 major complcation %2-4 2-After PEG mortality rate in 30 days : %3-23 I want to do multiple regression analyses . I have 2 dependent variables "mortality after peg " and "complications after peg" I have 31 independent variables. I do calculate at GPower . F test- Linear multiple regression : fixed model, r2 deviation from zero: A priori Effect size 0.15 , a 0.05 B: 0.95 number of predictors 31 Sample size 264. ***My question is this how can ı find effect size? Do you suggest me any other advice for my research sample size calculate ? Thanks for helping and sorry for baby english and long question.
Hi! As I am not a statistician, I cannot fully help. However, based on what I've understood of your question, you may be able to calculate the odds ratio for the percutaneous endoscopic gastrostomy vs. those without the intervention. This would give you the effect size parameter to input into the sample size calculations. For the regression analysis, perhaps a correlation coefficient would be the best way to assess it's effect. Please confirm theses approaches with a qualified statistician :)
Additional Essential Cell Culture Calculations (making up media and determining amount of drug treatment): ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-04y4Dl0uTA8.html
Hi Wendy, Yes, most individuals infected with HTLV-1 remain asymptomatic carriers throughout their lives. Only, a small proportion of infected individuals develop HTLV-1-associated diseases.
I'm not sure about this one but the two main clinical conditions associated with HTLV-1 are adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
Reference 2): Sequencing artifacts derived from a library preparation method using enzymatic fragmentation: journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227427&type=printable
Reference: DNA Library Preparation Methods for Next-generation Sequencing: Tone down the bias www.sciencedirect.com/science/article/abs/pii/S0014482714000160
References: 1) High rhesus (Rh(D)) negative frequency and ethnic-group based ABO blood group distribution in Ethiopia: www.ncbi.nlm.nih.gov/pmc/articles/PMC5530478/#:~:text=In%20Africa%20and%20Asia%20the,negative%20phenotype%20is%20even%20rarer. 2) Chapter 2: Blood group antigens are surface markers on the red blood cell membrane by Dean L. Bethesda (MD): National Center for Biotechnology Information (US); 2005. www.ncbi.nlm.nih.gov/books/NBK2264/#:~:text=Blood%20group%20antigens%20are%20either,the%20transfer%20of%20sugar%20units. 3) Blood groups (Better health channel VIC health) www.betterhealth.vic.gov.au/health/conditionsandtreatments/blood-groups# 4) Blood Groups and Compatibilities www.rch.org.au/bloodtrans/about_blood_products/blood_groups_and_compatibilities/
Hi Maame Adwoa, please i have a malaria vaccine to analyse the protein content. I have the Bradford Protein kit from thermofisher and the Albumin standards for the curve. can you help me with how to go about it?
Hello Alhassan, You will need make a 6-point serial dilution of a protein standard for which you know the starting concentration. e.g. Bovine Serum Albumin (BSA). You will then measure the absorbance of that standard against your protein of interest, in this case, your vaccine. If you have a plate reader, it may be also be easier assay it with that instrument (provided you have enough reagent for the required plate reader vol., typically 200ul). I have a video on here, that discusses how to approach protein determination so I'll link it below.
Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
The usual strategy is to leave the drug on for the entire 72hrs and assess following the designated time. However, it depends on the stability of the drug that you are assessing. If you know that your drug of interest is unstable and is completely degraded after say, 24hrs, then it may be wise to replenish.
hi thank you for your explaination. I have a question, if I measure dose dependency, in specific, I measure basal value of each well for 10 mins, and then add agonist and measure for 30 mins more. Then its supposed to have many points, then how should i choose value to make Z' factor?
I am a complete novice with ELISA but i need to run some blood tests that the UK does not offer, im going to buy all the equipment to conduct the following tests: MSH TGFB1 VEGF MMP9 C3A C4A Can you recommend a good book for the protocols and what elisa tests would be appicable for these tests (dircect/indierct etc.....) The top one is melanocyte stimulating hormone and i need to see a quantity not just sample presence in all of them Thank you, great videos
Hi @Rayshoesmith In terms of whether you perform a direct or indirect test, this will depend on how abundant the target is. If you suspect very low expression levels, then go for indirect. Regarding the melanocyte stimulating hormone where you need to see quantity not just presence, you will need to include a 6 or more point standard curve. This will help you report quantities. Regarding a book: I found this one online: ELISA: Methods and Protocols (Methods in Molecular Biology, 1318) 2015th Edition www.amazon.com/ELISA-Methods-Protocols-Molecular-Biology/dp/1493927418 All the best!
Thank you so much for this practical lecture for university students like me who cannot have the condition and biotechnological facilities to practice. I wish you all the best in 2024, Madam!
Why do we need to be able to do such calculations? In order to maximize all available storage space most solutions are stored in a concentrated form (known as stock). These solutions are then diluted to the required strength as and when required. This also means the same solution substance may be used for a different range of needed concentrations.
What is a good H-index? Hirsch suggests that after 20 years of research, an h-index of 20 is good! It means that you have 20 publications with at least 20 citations. 40 is outstanding, and 60 is absolutely exceptional.
I analyzed your channel and I was impressed to see your video quality. your video thumbnail is very eye-catchy. However, you are not getting proper views and subscribers. Below we found some problem for your channel - Your video title is not SEO friendly Don't have proper tags and keywords Don't have meta tags for video ranking Descriptions are not optimized Please fix this problems to get more views and subscribers
Other terms used to describe water quality in the lab includes: MQ/MilliQ water for type 1 water; Elix water for type 2 water and RO or demineralised water for type 3. Note that distilled water is water that is clarified through heating the water until it enters vapour form, and then cooling the water back to liquid form. Most labs do not use this method of clarification, preferring to use reverse osmosis, due to its greater efficiency.
Note: some labs avoid using antibiotics in their Media as they don't want to risk masking low level infection or worse, facilitate the emergence of antibiotic resistant strains. Get more comprehensive knowlege via this article from pubmed: www.ncbi.nlm.nih.gov/pmc/articles/PMC7149418/pdf/main.pdf
I want to use artificial intelligence to come up with guide RNA sequences and target sequences in Pam sequences. but i also want a way too verify that the artificial intelligence, aka chat gpt 4 is actually correct. What tools can I use to verify findings? Chop chop is incomplete and only provides target sequences etc.
