In this video we run samples on Agilent's Bioanalyzer to look at the nucleic acid distribution. First, take out the RNA dye concentrate, which is shipped to you and allow to equilibrate at room temp. for 30 minutes. We use the RNA 6000 Nano kit. You will need the gel, make a dye-mix, and use the marker and ladder shipped with the kit and agilent's Bioanalyzer instrument and software.
After preparing the gel and loading the RNA samples, open the instrument by lifting the front Metallic silver colour part of the instrument. Once lifted gently close then open the software and select the correct chip that you about to run. The agilent bioanalyzer instrument enables automated electrophoresis of DNA, RNA and protein. There are different reagent kits depending on what you’re trying to do. So, for RNA, for example, you may buy the bioanalyzer RNA 6000 PICO kit. This is if you have very little amount of RNA that you expecting. e.g. as little as 50 picogram of total RNA. You can use it for your total RNA, as well as your mRNA. So the RNA 6000 NANO assay kits are for characterising total RNA samples in the nanogram range. E.g. RNA extracted from micro dissections. If you’re working with small RNA species, there is a dedicated kit that you can use for that.
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This video is about Bioanalyzer 2100, Agilent Bioanalyzer, Electrophoresis analysis, DNA/RNA analysis, Protein analysis, Microfluidics technology, Lab-on-a-chip system, Gel electrophoresis alternative, Bioanalyzer protocol, Software walkthrough, Data analysis software, Quality control in genomics, Nucleic acid sizing, Automated electrophoresis and
Lab workflow optimization
15 янв 2024
