Viruses are suggested to be the most abundant and diverse biological entities on our planet, with an estimated 10^31 particles on Earth.
While it's been demonstrated that the human body hosts vast microbial communities (termed the microbiome), the fact that the human body also hosts vast numbers of different viruses, is less well known. The collective community of viruses in a given environment is called the 'virome'. mammalian viral families in rodents
Virome refers to the assemblage of viruses[ that is often investigated and described by metagenomic sequencing of viral nucleic acids that are found associated with a particular ecosystem or organism . The word is frequently used to describe environmental viral shotgun metagenomes. Viruses, including bacteriophages, are found in all environments, and studies of the virome have provided insights into nutrient cycling,development of immunity,[6] and a major source of genes through lysogenic conversion.
Some scientists are interested in understanding viral communities present in wildlife, as well as the prevalence, genetic diversity, and geographical distribution of these viruses, as it could be valuable for prevention and control of wildlife-origin Emerging Infectious Diseases. Comprehensively studying the faecal viromes of laboratory rats under different dietary conditions could also be useful in understanding how the virome affects health.
In this video, we go over the preparation of viruses (bacteriophages) from enteric bacteria, followed by DNA extraction and next generation sequencing for studying metagenomes, viromes, viruses, gut virome, viral infections, etc.
Typical enteric viruses found in rodents include hantaviruses, mammarenaviruses and coronaviruses.
16 окт 2024