Hello, Welcome to quick biochemistry basics. The videos in this channel are short and to the point, with few animations, that allows you to grasp the basic concepts of biochemistry and molecular biology quickly.
Thanks for breaking these steps down in a ways I can understand. I truly need to understand very well in this enzyme process-I cannot thank you enough.
Quick Summary: The Meselson and Stahl experiment provided experimental evidence for the semi-conservative DNA replication hypothesis proposed by Watson and Crick. Meselson and Stahl's 1958 experiment proved DNA replication is semi-conservative by using N15 and N14 isotopes, showing heavy DNA as a template for new strands.
Ion exchange and SEC both are HPLC instrument just connecting size exclusion column and cation exchange or anion exchange column we have to use. Is it correct?
Every one Read this please. Doubt clear Ion exchangers are either cation exchangers, which exchange positively charged ions (cations), or anion exchangers, which exchange negatively charged ions (anions). There are also amphoteric exchangers that are able to exchange both cations and anions simultaneously. The resins consist of aggregations of big insoluble molecules carrying fixed negative charges, which loosely bind positively charged ions (cations); these readily exchange with cations in the fluid environment to an extent that depends on their affinity for the resin and their concentration. In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.
