U r awesome

Awesome!

Very helpful and clear! Thank you so much

An absolute gem! Thank you so much!

This is a fantastic explanation; thank you so much!

I can’t believe someone can explain this so simply while being detailed! Thanks Elmer! 👍

The best one I understood from him the plasmid extraction Thank you I watch you from Egypt ❤

Please make more videos on base editing andcprime editing ❤❤❤plzzz

I cantvthank you enough for teaching it in wonderful way...love from India❤

Very nice dear sir

what is the percentage of your agarose gel? I read that we need to have it at a higher concentration to see the separation

🙏

I just subscribed and binged watch alot of your videos. Please keep making more amazing videos. I'm extremely impressed and grateful that I've found your channel. Thank you very much!

Very useful and easy to understand... Requesting for more videos

Your videos are life saving! Thank you

bro How to add or remove Stop codon

Hi Jacob ' you did a brilliant presentation ' however i'm a beginner and still confused.Although it's not my field specifically but i need to choose a short dsRNA sequence of 20 or 27' 28 nucleotide sequence for application in plant but i am not familiar the specificity of the sequence if we had a genome like choloroplast genome and split that sequence into primers can we choose the shortest sequence ? Wether it will work or not??

Really great educational video

Hi Jacob, thank you so much for this video. It’s really helped my work and I appreciate your time in sharing! I have a couple of questions if you could answer them please I’d appreciate it 1. What is the vector to oligo ratio that you use for annealing and 2. how much ATP do you use?

Hi, first of all its a very helpful video. Could you please explain why did you consider only 1500 bp while adding the sequence range in Eukaryotic Promoter Database?

SIMPLY PERFECT

Very usuful and exhaustive for me. Thank you

Hi. Do you have any information on the database for Transcription factors for Filamentous fungi such as Trichoderma, and Aspergillus as PROMO doesn't contain these species?

Thank you for the informative video. My question is about ScRNA to which Cas9 binds. gRNA along the ScRNA sequence should be cloned in the vector, right ?

I liked what you showed in the V2 Galaxy. You mentioned there was coming videos after that. I can't find any so far. Is this true? Many thanks.

Wow why did i just find this channel? It seems great!

Hi Jacob why there is no signal peptide in the NCBI and no annotation available in UNIPROT too. In that case can I select the Exon 2 as a target?

Excellent explanation.

Flibe! Elysium was cyber attacked by UK into bankruptcy and Sale. Just like Rudy Giuliani and other Thorium players Norway. 🇳🇴 My neighbor , who died while I was blacklisted. This science safety excavation matches your Uranium 0.05% burn ratio without the Liquid Flouride Thermal Reactions. Basic Training-USA military does not cyber protect civilians! I’m case in point! #KING #POW18.04.2567.001 #HHVV #VVHH

such a great videos i read so many papers but nobody explained this clearly

Everyone who learned from this video should always be awared, especially those who are designing experiments related to DNA, genome or chromatin... NOT ALL the transcription start sites are in the first exon of the gene and also MOST of the genes contain intron in DNA level...

Amazing video easy to understand and you have a really nice way of explaining ! Keep up the good work

9:30 the entire transcript sequence.

3:20 Promoter active , recruiting r n a polimerates to transcribe genes, encourages binding

Thank you for awesome video. Is there any website to design gRNAfor Cas3 CISPR system?

Great video🙌🙌 I have one question? l am interested in mutating a PTM site of the proteins. In that regard can we use CRISPR to change a particular base in DNA such that the gene expression is not affected but that codon now encodes different amino acid. That new change should not effect protein structure and function Is this even possible?

This video has been a life-changer for me. I think every view of this video resulted in a scientific paper. You deserve credit for each one of them. This is a prime example of how science grow. Thankyou.

very good video. Easy to understand such a difficult approach.

So beginner friendly and helpful. Thank you so much! ♥️

anyone attempting CRISPR point substitutions? not knock-out/in :(

Thanks Jacob that was well explained and thorough.Cheers Sarah Nyondo also a Laboratory Technician.😄

Very informative video. Clear all doubts about gRNA design.

WOOWW, alot of learning. Highly appreciate it. many thanks

Thanks again for this super duper video I have an inquiry please In 6:43, you said that Cas9 is pretty faithful but it can mistakes so its best if you only use gRNAs that have at least 3 mismatches and in 16:30, you said its better to have a gRNA with no off targets and zero mismatches, its important that MM0 be zero as if this number greater than zero, the gRNA will cut my target gene and some other place in 16:50, you said that its very difficult to find a gRNA that has zero off targets with 3 mismatches I did not understand this part, sorry if its clear and I didn't get it, but I found myself confused

Thank you very much! I would appreciate if you might add a comment on how to proceed if for my gene of interest I see - strand in the EPD? Should I invert the sequence to get the correct promoter?

Thank you!

Thank you so much. The way you explain by breaking everything down makes it so easy to understand. I hope you make more videos like this. Also, your way of articulation and slides are amazing and never bores me out.

Great video! Thanks

Awesome!

Thank you ❤