How to design primers that will amplify a promoter from the human genome, which you can then clone into an expression plasmid using restriction enzymes and a DNA ligase.
Ok man your video was cool, but i was expecting more about primer design for restriction sites. I came up on this video looking for the difference between linkers and Oligos designed to add restriction sites of our interest's insert, and to be honest i didn't find it in your video. Good quality tough. Regards