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Designing PCR Primers for Restriction Enzyme-mediated Cloning 

Jacob Elmer
Подписаться 7 тыс.
Просмотров 21 тыс.
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How to design primers that will amplify a promoter from the human genome, which you can then clone into an expression plasmid using restriction enzymes and a DNA ligase.

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1 окт 2024

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Комментарии : 9   
@danieltito1234
@danieltito1234 3 года назад
Ok man your video was cool, but i was expecting more about primer design for restriction sites. I came up on this video looking for the difference between linkers and Oligos designed to add restriction sites of our interest's insert, and to be honest i didn't find it in your video. Good quality tough. Regards
@lolohsn2515
@lolohsn2515 2 года назад
Good video Dr. elmer ! but how did we design primers with R.E sites for new promotor
@nadaj.9943
@nadaj.9943 4 года назад
That was really helpful, thanks!!
@adwoabiotech
@adwoabiotech 4 года назад
Exceptional walk through - thank you⭐⭐⭐⭐⭐
@elissaalam6724
@elissaalam6724 4 года назад
That was great, thanks!
@rahelehroudi7379
@rahelehroudi7379 3 года назад
My aim is to replace the EF1-A promoter in the pT3-EF1α plasmid with CLEC4F promoter. Please guide me in this regard.
@cacevedo07
@cacevedo07 2 года назад
This Man should have a million subscribers! Many thanks for your videos.
@Milo-tl9oq
@Milo-tl9oq 2 года назад
you are awesome. Thanks for your time!
@aryarezayi8622
@aryarezayi8622 10 месяцев назад
Thank you ❤
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