🧈 How to do Lipid Extrusion (Nanosizing Liposomes from LMV to SUV) || Practical Biochemistry

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if your lipids are in chloroform, evaporate with nitrogen gas. freeze dry overnight, hydrate with water, freeze thaw cycle a couple times, freeze and freeze dry to get my starting lipid form.
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8 сен 2024

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Комментарии : 18

@sridevib8195 2 года назад

You are a goddess. You dont have any idea how much of help and service you are doing to research fellows like us. Wish you all the health and success.

@biochemistmelo 2 года назад

Thank you 😊. I really hope they are helpful.

@ayahjabateh6339 2 месяца назад

Do you store lipid stocks by aliquoting them to prevent melting the entire bottle before weighing? Does this method help maintain the integrity of the lipids? Alternatively, can they simply be stored at -20°C?

@samuelwamwere4867 Год назад

It was just yesterday I heard about this technique and now I will understand what I will be reading. Thanks

@biochemistmelo Год назад

Glad it was helpful 😊

@chirasmitadas4494 2 года назад

Great explanation. May you please make a video. How to prepare liposomes by thin-film hydration method.

@biochemistmelo 2 года назад

Thank you. Yes definitely.

@PoonamYadav-gh3fy 11 месяцев назад

Can you please share the details (product details and catalogue number) for the extruder you used?

@aishaar2726 Год назад

How much is this hand extruder in dollar please, and where can i get it online with cheapest price? Also is there a plastic one that is cheaper?

@biochemistmelo Год назад

I think around $1000 but they are standard and have a mix of metal and plastics in them. Ours is from Avanti Polar Lipids (extruder kit)

@chirasmitadas4494 Год назад

I am using same extruder but it is new,I have brought it 6 months before.but leakage in one syringe,any tips? Please maam

@biochemistmelo Год назад

Hi dear. Leakage is usually from syringe. Is your syringe new too?

@nadakhaled8839 Год назад

I am using the same extruder but one problem I am facing is that the sample keeps leaking from the side I am pushing it from it doesn't pass to the other side, any tips?

@biochemistmelo Год назад

This often happens when older glass syringes are used. It happened to me a lot too. Replacing the syringe for us fixed the problem.

@mahmoudaltweasi3652 2 года назад

What is the purpose of this step?

@biochemistmelo 2 года назад

This is extrusion. Converts liposomes from large multilamellar vehicles to small unilamellar vesicles. Any sort of study involving proteins bound to or inserted into lipids requires this to be done first to the lipids. Otherwise there will be multiple lipid bilayers like an "onion".

@user-un2sn2yb6r 9 месяцев назад

I would highly advise you wearing gloves at all times and following a more detailed cleaning procedure. From my experience this will ruin you quite some work you are doing here.

@biochemistmelo 7 месяцев назад

I've been doing this for 8 years and always check my liposomes with TEM. I wear gloves when I'm working because if I didn't I would see it on the TEM. You should worry about yourself.