1) Adeno Associated Virus (AAV) - An Introduction

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Adeno Associated Virus (AAV) is a new gene delivery system that is ideal for gene therapy. They are small and relatively simple viruses that have broad host range, and their apparent lack of pathogenicity and low immunogenicity increase their potential as viruses for gene therapy.
Adeno-associated viruses offer numerous advantages over Lentivirus and Adenovirus gene delivery systems. The numerous AAV serotypes allow viral infection in virtually all tissue types, and its ability to infect both the dividing and quiescent cells makes them a great gene delivery tool for genome editing applications, such as CRISPR-Cas9 knock out. In this video, we will introduce some key features of adeno-associated viruses and briefly and go over its pros and cons as a genome editing tool.
For more information on adeno-associated viruses (AAV), please visit:
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➜ Next Generation Sequencing: • 1) Next Generation Seq... .
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Опубликовано:

30 сен 2024

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Комментарии : 33

@kathyc3005 7 лет назад

Great explanation, thank you so much! I wish it was possible to mute the music.

@abmgood 7 лет назад

Hi Kathy! Thanks for your comment! We do have closed-captioning if you wish to mute the music. Regarding the music sound level, we have taken your comment into consideration - our newer videos have less intrusive background music.

@TheGanjam 7 лет назад

Excellent video. Many thanks. Could you please also add a video on how to quantify AAV by real time pcr step wise. Cheers ;))

@abmgood 7 лет назад

Hi there, thanks for watching our video and commenting! We can certainly put your request on our list of future video plans - thanks for the suggestion!

@izzaimran9419 8 лет назад

itd be really nice if there were subtitles for this video!

@abmgood 8 лет назад

Hi Izza Imran, thanks for watching our video! If you click on the "CC" button (bottom right of the video), you should be able to switch on closed-captioning for the video (the button will appear with a red line underneath it). Thanks for your comment and hope you enjoyed the video!

@tinacole1450 Год назад

excellent

@sangeethvaheesan3173 Год назад

Great video, very simple and to the point, makes researching further a simple process without complicating the process. Thankyou.

@abmgood Год назад

Thank you for your feedback!

@goes. 5 лет назад

So is the transgene delivered in the adenovirus or the AAV? is the only genetic material in the AAV the rep and cap proteins?

@abmgood 5 лет назад

Hi Gabriel, the video describes AAV as it was discovered and its interactions with Adenovirus. When we are looking at using packaged AAV virus carrying a transgene for transduction, the genetic material carried by AAV consists of the payload (typically a promoter and a transgene) flanked by the ITR sequences. The Rep and Cap proteins are expressed from another plasmid during packaging, but this plasmid does not get packaged into the AAV itself because it lacks the ITR regions which are only on the plasmid carrying the transgene. This provides an additional level of biosafety, requiring the packaging plasmids to be present in order for the virus to replicate.

@LingCamillo-e9p 20 дней назад

Charity Grove

@mauriziocelentano7658 Год назад

The background music is annoying and covers the speaker's voice.

@bdhome2010 5 лет назад

I want to explore genetic engineering from basic to research level. Can you provide me a guideline?

@abmgood 5 лет назад

If you're interested in exploring genetic engineering using CRISPR, we have a CRISPR knowledge base that starts off with introductory material (www.abmgood.com/marketing/knowledge_base/CRISPR_Cas9_Introduction.php) and later goes into more details regarding experimental design. We also have a CRISPR Crash Crouse (a 4-week online course) that's free to join at info.abmgood.com/crispr-crash-course, and the materials are geared towards prepping you to performing your CRISPR knockout experiment.

@NamasenITN 2 года назад

Audio is suboptimal and English pronunciation is not always very clear.

@dramirfarhadbahreini6477 3 года назад

Waw I appreciate the luciferase enzyme!!!!!!! Dr Farhad Bahraini from Russia academy of medical sciences

@aswang7890 5 лет назад

In the tropism slide, you verbally mention AAV 9 but did not describe its tropism.

@abmgood 5 лет назад

You're right - sorry! We cover it in our AAV Knowledge Base though: www.abmgood.com/marketing/knowledge_base/Adeno_Associated_Virus_Introduction.php#TAS (See Table 1 - Table of AAV Serotypes and their respective Tropisms.)

@aswang7890 5 лет назад

@@abmgood Thanks for the link. You have a typo on Parvoviruses in the Intro.

@abmgood 5 лет назад

@@aswang7890 You're welcome, and thanks for catching that. We've fixed it now :)

@孙梦茹-o9j Год назад

Great video

@hxzllj 4 года назад

Who else is here because the odd1sout

@calebthepro2676 3 года назад

@xzaviormilton4111 Год назад

Can you do gene therapy with influenza a virus vector ?

@vitorboico7050 8 лет назад

Its very nice!!!

@abmgood 8 лет назад

Hi there! Glad you enjoyed our video -- thanks for the comment! Let us know if you have any questions!

@userou-ig1ze 4 года назад

please mute music. please don't confuse shavers and microphones. Thx, the internets

@abmgood 4 года назад

Hi there, thanks for watching and leaving your feedback! Yes, this was an older video we produced and we have since taken your feedback and others' and improved our sound quality. For this particular video, you can also mute and turn on the captions for a slightly better experience.