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1) PCR (Polymerase Chain Reaction) Tutorial - An Introduction 

Applied Biological Materials - abm
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This PCR introduction will demonstrate that PCR is a fundamental technique used to amplify fragments of DNA, frequently using the Taq polymerase to facilitate the amplification during the thermal cycling process.
There are many variations of the PCR process including RT PCR for reverse transcription from an RNA template, qPCR for quantitative real time PCR to quantify the amplification during the chain reaction, and many more techniques to optimize the process.
In this PCR video animation, we will give you an overview of the polymerase chain reaction steps as well as the role Taq DNA Polymerase plays in the PCR process. You will learn about PCR applications and factors important in the design of your PCR experiment.
For more information on Polymerase Chain Reaction (PCR) and for a list of the sources used, please visit:
➜ Knowledge Base: info.abmgood.com/polymerase-c...
Thank you to all of the amazing community contributions towards translating this video into other languages including:
➜ Indonesian
(To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.)
Check out our other video series:
➜ Polymerase Chain Reaction (PCR) - An Introduction: • 1) PCR (Polymerase Cha...
➜ CRISPR Cas9: • CRISPR Cas9 - A Brief ...
➜ Adeno Associated Virus: • 1) Adeno Associated Vi...
➜ Cell Culture: • Cell Culture
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
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➜ Twitter: goo.gl/gGGtT9
➜ LinkedIn: goo.gl/kSmbht
➜ Google+: goo.gl/5bRNwC

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10 дек 2015

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Комментарии : 75   
@electronic5metallica
@electronic5metallica 3 года назад
Well, that background music is going to be stuck in my head all day lol
@anhpham6988
@anhpham6988 6 лет назад
Why all my professors failed to explain simply like this? Thanks a lot
@abmgood
@abmgood 6 лет назад
Hey Anh, thanks for leaving a comment! Glad to hear that the video was helpful :)
@ukkepukkie2882
@ukkepukkie2882 3 года назад
Very well animated video! It makes everything much easier to understand. Thank you for posting this!
@abmgood
@abmgood 3 года назад
Hello Alyssa, Thanks for watching! Glad to hear that you found the video helpful! :)
@mosesomungo6540
@mosesomungo6540 5 лет назад
Hi, the lecture is quite nice and very clear thank you for the excellent PCR tutorial link.
@DrAbdelmoneem
@DrAbdelmoneem 7 лет назад
Really great presentation . A huge thumb up from Egypt :D
@akinyemikindnessoluwole3352
@akinyemikindnessoluwole3352 8 месяцев назад
THis is my first clear understanding to PCR Am very impressed Thanks a bunch
@jawabtvdigital
@jawabtvdigital 4 года назад
RU-vid is becoming a teaching tool more advanced than previous
@Mwahidah
@Mwahidah 5 лет назад
This was very helpful. Thank you!
@abmgood
@abmgood 5 лет назад
You're welcome, Zay! Glad to hear that the video was helpful.
@andiehangula
@andiehangula 4 года назад
this video is everything
@nathanrumpf5660
@nathanrumpf5660 8 лет назад
Great video. Thank you
@abmgood
@abmgood 8 лет назад
Thank you Nathan, glad you enjoyed it!
@buharimuhammad5989
@buharimuhammad5989 6 лет назад
Awesome video tutorial!
@abmgood
@abmgood 6 лет назад
Hi Buhari! Thanks for the comments and glad you enjoyed our video. Let us know if you have any questions - we'd be happy to help!
@kenethiceland5271
@kenethiceland5271 6 лет назад
Thanks for the extra notes....quality control points...priceless...Hokudai
@abmgood
@abmgood 6 лет назад
Thanks for watching, Keneth!
@filipbuczynski9905
@filipbuczynski9905 5 лет назад
Thank You, it is really useful
@abmgood
@abmgood 5 лет назад
You're very welcome, and thanks for your comment!
@violet12327
@violet12327 6 лет назад
Great explanation!
@abmgood
@abmgood 6 лет назад
Hi Koem, thanks for watching and leaving a comment! Glad you enjoyed our video - please let us know if you have any questions and we'd be happy to help answer them.
@sindyayukirana3508
@sindyayukirana3508 4 года назад
Hi Applied Biological Materials Thank you for this great video. I'm from Indonesia
@marwamokhtar8469
@marwamokhtar8469 5 лет назад
Great video , many thanks 😅
@abmgood
@abmgood 5 лет назад
You're welcome, Marwa. Thanks for the nice comment!
@gaylenailo713
@gaylenailo713 6 лет назад
very helpful, thanks
@abmgood
@abmgood 6 лет назад
Hi Haley, thanks for leaving a comment! Let us know if you have any questions!
@dongwu8499
@dongwu8499 4 года назад
thanks a lot! this video is quite helpful the picture is vivid. Hopefully, more and more great instruments will be invented by your company.
@abmgood
@abmgood 4 года назад
Thanks for watching! We are really glad to hear that you found this video helpful. More interesting videos are coming so stay tuned!
@RB-nr8rc
@RB-nr8rc 4 года назад
Ditching school and learning from RU-vid
@abmgood
@abmgood 4 года назад
Thanks for watching! :)
@user-rg6up7co4j
@user-rg6up7co4j 7 лет назад
amazing presentation
@abmgood
@abmgood 7 лет назад
Hi Deepthi, thanks for your comment and watching our video! If you want to learn more about PCR, we do have a knowledge base that explores it in more depth: goo.gl/OAuLRV
@shivamlal8636
@shivamlal8636 7 лет назад
awesome video .
@abmgood
@abmgood 7 лет назад
Hi Shivam, thanks for your comment! Glad you enjoyed the video!
@snehajpraj9100
@snehajpraj9100 5 лет назад
Thank you for the video....
@abmgood
@abmgood 5 лет назад
You're welcome :) Hope you found it helpful!
@naginakhan1ify
@naginakhan1ify 5 лет назад
Thank you so much
@abmgood
@abmgood 5 лет назад
You're welcome! :)
@user-cx3dy5ud8h
@user-cx3dy5ud8h 7 лет назад
amazing! i'm a student from Korea, and I'd like to watch this video in Korean, too. i'll very thank you if you try to make this great video about pcr in multiple languages. XD
@abmgood
@abmgood 7 лет назад
Hi there! Thanks for watching and leaving such a nice comment! Yes, currently our videos are only officially translated into english and mandarin. However, youtube has an automatic translation of captions (if you click on the gear icon in the bottom right corner of the video > click "subtitle" > click "autotranslate" > select your language) which you might find helpful!
@maxdadax
@maxdadax 5 лет назад
Thank you :)
@abmgood
@abmgood 5 лет назад
You're welcome :D
@panorama85
@panorama85 Год назад
I like your unique style of teaching. Nice and detailed explanation. Thanks from Bangladesh 🇧🇩
@abmgood
@abmgood Год назад
You are most welcome :)
@sophaagitations3339
@sophaagitations3339 6 лет назад
lit.
@abhilasha9608
@abhilasha9608 4 года назад
This is better than the modules they show at school
@abmgood
@abmgood 4 года назад
We are really glad to hear the video was helpful. Thanks for watching!
@waqassalim3530
@waqassalim3530 7 лет назад
fantestic video
@abmgood
@abmgood 7 лет назад
Hi Waqas, thanks for watching! Let us know if you have any questions!
@GladiaTheDark
@GladiaTheDark 5 лет назад
I wonder what’s the name of the music track in the background. So funny!
@abmgood
@abmgood 5 лет назад
Hi, it should be a track from RU-vid's audio library.
@herrmanselcher1632
@herrmanselcher1632 3 года назад
Number 15 Polymerase Chain Reaction
@santhyjulia5032
@santhyjulia5032 4 года назад
Thanks. I'm from Indonesia
@abmgood
@abmgood 4 года назад
Thanks for watching!
@dxlabs
@dxlabs 4 года назад
Bisa saya kontak?
@santhyjulia5032
@santhyjulia5032 4 года назад
@@dxlabs iyaa ada apa pak?
@aliahzar8240
@aliahzar8240 3 года назад
Question: how can we know if a sample is more rich in GC or in AT?
@abmgood
@abmgood 3 года назад
Hello Aliah, There are tools such as a GC content calculator to analyze the % in a given template sequence. Simply Google "GC Content Calculator" and paste your template sequence into the tool to find out the percentage.
@MediaBuster
@MediaBuster 4 года назад
Why don't you show actual video of all of this?
@lintaali6784
@lintaali6784 4 года назад
How we get our desired product in pcr if long ends are produced?
@abmgood
@abmgood 4 года назад
Hi Linta, Thanks for watching and leaving your question! Would you mind clarifying your question or if the question is in response to a section in our video? If you would you like to amplify long PCR amplicons there are DNA polymerases specifically engineered to do this with high specificity (such as abm's Long-Range DNA Polymerase)!
@lintaali6784
@lintaali6784 4 года назад
Thanks for replying .long fragments are produced but in low quantity and that's why they don't show on gel
@abmgood
@abmgood 4 года назад
​@@lintaali6784 Yes, you are correct - in low quantities, they won't show up on the gel. To amplify them, you can use the DNA polymerases specifically engineered for long PCR amplicons, as mentioned in my last comment. Let me know if you have other questions!
@aesthetic8307
@aesthetic8307 3 года назад
Just came beacause of module
@hifzamehak4211
@hifzamehak4211 3 года назад
nice vedio,,, but background music is effective
@abmgood
@abmgood 3 года назад
Hello Hifza, Thanks so much for your feedback! We have made improvements on our recent videos. Please check them out too ;)
@user-kk3xd6jr6z
@user-kk3xd6jr6z 4 месяца назад
link error: 404 not found
@dellaroccia
@dellaroccia 3 года назад
great information and presentation, still - audio background sucks
@abmgood
@abmgood 3 года назад
Thanks so much for your input!
@isolderayman-moore7891
@isolderayman-moore7891 3 года назад
yall may be here from biology but im here from forensic science ;)
@rooroo3199
@rooroo3199 3 года назад
Great video but please remove background music, very distracting!
@abmgood
@abmgood 2 года назад
Hello there, We are well aware of this issue but unfortunately we couldn't replace all the old videos. However, we have made improvements on our latest videos so please check them out! :) As an alternative solution, you can always mute the video and turn the caption on if the background music bothers too much :) Thank you!
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