Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 2 Instructor: Eric Lander View the complete course: ocw.mit.edu/7-01SCF11 License: Creative Commons BY-NC-SA More information at ocw.mit.edu/terms More courses at ocw.mit.edu
This is how science should be taught. Teaching the experiments that led to the concepts rather than just teaching the concepts themselves without any context
5 дней назад
He is the master of genomic knowledge. The legendary Eric Lander, the director of Human Genome Project.
Thank you for this wonderful class. I wish there were qualified teachers as well here in Brazil, because my molecular biology's classes were very disappointing and I could only understand the subject after seeing this video. Thank you so much, success sir Eric Lander!
I'm as jealous as i am grateful that i don't have Professor Lander asking me questions in class... I'd clam the hell up quick smart. Such great lectures
Many thanks. This series is amazingly helpful. Just a little suggestion. I turn my speaker to its highest volume, it is only at an acceptable level to listen clearly. So maybe a little improvement in recording can help.
20:14 having a stop codon in an intron would still be a problem because the polymerase would stop before finishing the transcription of the whole transcript wich is then supposed to undergo splicing
First question: where does the primer come from? Answer to the first question: insert it to a vector which has a different specific RE cut site a couple of base pair upstream to the gene of interest and use that sequence as a primer.
Second question: if we have to sequence a long gene for example has 3000 bp, we have to use a longer primer? Answer: can do primer walking or can cut the 3000 bp gene up into smaller fragments and subclone these fragments and do overlapping assembly aka shotgun sequencing
How do you know you recognize a gene: look for open reading frames (this is done for not complicated species) or we can make a cDNA and then compare the sequence of your gene of interest with that of another organism
4th question: how do you get a gene of interest from a second individual from the same species(aka you know the sequence of the gene of interest of individual A): do PCR!
Primer is just a small chunk of RNA which are complementary to the original DNA. Since we know the DNA sequence, we know the Primer sequence, type in the automatic synthesis machine and BOOM you have your Primer.
these are great but i have difficulty navigating the series, is there somewhere where these lectures are combined or sequenced such that i find the next lectures?
Is it really true that stop codons occur in introns? How would that work? Does the ribosome know that it is transcribing an intron and therefore ignore stop codons? I don’t get how this would work to be honest. Other than that I would like to say that I truly envy those who get to take Eric Lander’s classes, he is one of the best biology teachers that I have ever seen.
I think it's too late now, but anyways 😂 We know it's a sequence recognised by a specific restriction enzyme, if we know what enzyme it is and we know the sequence it recognises we just use that same sequence as the primer
The following textbook was used when this course was taught on the MIT campus: Campbell, N. A., J.B. Reece, et al. Biology. 8th ed. Pearson Benjamin Cummings, 2008. ISBN: 9780805368444. For more information see the course on MIT OpenCourseWare at: ocw.mit.edu/7-01SCF11.
his schtickt:::: REPEAT YOURSLEF ...... AND THEY ARE ALL FUNCTIONING ON THAT EXACT SAME SORRY LEVEL -----?>>>>> SOMETHING TELLING TO THOSE 'SORY' DYNAMICS
