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Alkaline Lysis Miniprep 

Bio-Rad Laboratories
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This video demonstrates the alkaline lysis method of purifying plasmid DNA from a bacterial culture. The small scale purification is also known as a mini prep. This method utilizes a microcentrifuge as part of the purification.
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12 сен 2024

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Комментарии : 7   
@abdulrahmanibrahimdansadau6774
@abdulrahmanibrahimdansadau6774 9 месяцев назад
This is 11 years this vedio Was made, I find it very useful today. Thank you bio rad
@bryantchan4251
@bryantchan4251 4 года назад
very useful, thank you!
@ashutoshkosariya191
@ashutoshkosariya191 3 года назад
Could you plz provide protocol for this experiment
@tiggertiggatiger
@tiggertiggatiger 11 лет назад
wow! this is super detailed! thankyou!
@scottfabricant7140
@scottfabricant7140 10 лет назад
The washing solution used came from the bottle saying 5x, and also says "alcohol added". Was the washing solution diluted down to 1x before use? Why the (unnamed) alcohol, is this (presumably 100% ethanol) used to make it 1x?
@scottfabricant7140
@scottfabricant7140 10 лет назад
Also, try saying "elution solution" as fast as you can ten times. See how far you get.
@thestormcraft4012
@thestormcraft4012 6 месяцев назад
most of the time the ethanol content used for these is 70%
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