Antibacterial Activity Test by Disk Diffusion Method_A Complete Procedure (Kirby and Bauer Method) 

Your channel is very nice I am thankful to you as i recalled all the methods i forget, by watching your videos.

Very Helpful. Thank you.

Thank you for videos such as these! You are the best. Learning a lot from your videos. Can you do Antimicrobial protection (Preservative efficacy) test for cosmetic samples? Thank you so much and keep up the good work. More power to you.

Thank u so so much for such an amazing information

Nice handling...

For thoes who don't know we can use either one Nutrient agar or muceller hinton agar or Tryptone soya agar for in the petri dish

Really very good very easy and simple way

Excellent demonstration and explanation. Pl continue with nanobiotics

Awesome thanks

Well done 👍

wonderful

Thank you

thank you

SUPER COOL

Very good

Sometimes it happens that the zone of inhibition isnot very clear, 2 shades are present,qhy does it happen and what should bedone,how should we measure the zone of inhibition me

Hey I'm working on a fabric treated with copper nanoparticles (turns out I actually synthesised copper *micro*particles but let's not talk about that). Do you think I can use this method to test the antibacterial activity? I've been searching for methods and was thinking between ATTCC 147 and JIS L 1092 but I've figured out my fabric samples were not fit with aatcc 147 and I couldn't find enough information about jis l 1902. I would appreciate some help :)

Educative

Hi, thanks for the great video. I am trying to culture Pediococcus acidilactici on MRS Agar and see its resistance to a certain compound. How can I make a paper disc specific to the compound I want? Do I just dip the paper into my mixture?

This video was very helpful. However, I wanted to ask about the sample used and if you know any other samples that can be used in this kind of experiment.

Hi, Nice video. I am totally naive in this. I want to check the antibacterial activity of silver thin films and small scaffolds. Will you please guide me? Is it necessary to use Amoxycillin antibiotic disk along with our sample?

Share Antibacterial activity of CuO nanoparticles plz

Hi! I am still quite naive in this topic. I have two questions I wanted to ask : 1. if I am testing an antifungal activity of a substance (garbage enzyme from citrus peels to be specific), can I use well diffusion method? 2. do I inoculate the fungi in the same way as shown how to inoculate the bacteria in the video (by streaking the entire surface of the plate)? Or is there a better way to do it with fungi (ascomycete)? I am using Botrytis cinerea as the fungi btw. From what I can understand so far (by researching the topic on the internet), the standardized/more reccomended method for testing against fungi is to use broth dillution, but I am also under the impression that well diffusion can also work to test antifungal activity againts Botrytis cinerea, from a study I had found titled "A Comparative Study of Antimicrobial and Antioxidant Activities of Plant Essential Oils and Extracts as Candidate Ingredients for Edible Coatings to Control Decay in ‘Wonderful’ Pomegranate" Sidenote : thank you so much for the content you post here, as a student I find them extremely useful. Please know that is perfectly understandable if you don't find the time or need to respond to my very specific questions, and sorry for taking your time 😄

Could one use a block sample of a filter paper treated with a sanitizer

Hi,, your video is so nice.. im here to know what method should i use to to test the effectiveness of anti bacterial hand soap that we are to going to make. Can you please share me a tip or method that i should use to determine if the antibacterial handsoap that im going to make is really effective for hands and how would i make it.(school research purposes only) your reply would really be appreciated... Thank youuu

Hello! I've got a fabric treated with silver nanoparticles. Do I need to sterilize my samples before I test the antibacterial activity? If so, how do I sterlize them?

Hi there, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?

how about antifungal activity for seaweeds against m.fijiensis can be the same method? also the agar will be PDA

@MicroChem's Experiments How can someone do the Antibacterial Activity Test for a powdered sample for example termite sample?

how did you made the pure culture plate of staphylococcus aureus?

How to do antibiotic susceptibility test for Onion..can you make a video

Dear Madam..u r doing well job but if u will explain addition of chemical.i.e why we are doing this chemical and reason behind it than it will be more helpfull to all..tx a lot

Hi can u please show any video for antifungal activity by well diffusion method

Hi! where did you use the TSA?

Dear ma’am waiting for fungal susceptibility test especially for black fungii

Ma'am how do you prepare the pure culture colony of bacteria?

What was the purpose of preparing Trypton soya agar? Or I'm missing something. Plz help! Overall video was very very useful. Thanks a lot and keep going.

Nice video😍 one question though What if the sample is liquid ?pls help me out 🥲🥲

Can I perform the same test for anti-microbial activity ?

Plz phasforus test in laboratory plzz

Maam why we are using 2 types of agar here...and what is a nutrient agar ..are these examples for nutrient agar? I belong to chemistry background and dont have any idea about this method..but i need this method for my project .

Hello! I know this comment is kinda late, but can this study use for testing of the effectiveness of antimicrobial gel of band aid?

Is it necessary to use MUELLER HINTON AGAR

Maam, how much agar that we can make with 38g of MHA medium ? in the video, i only can see 1 complete agar plate? if i need to make about 8 agar plate, how much do i need to use? Thank you in advanced

Maam I have made antimicroboal herbal handwash and now howI will check its antimicrobial activity

Pls can TSA be used as the pure culture instead of TSB

How to make a streptomycin disc? Please help me

Ma'am, can you please let us know how the block of the sample was prepared?

I still waiting for analysis video Of sugar percentage in any samples Please 🙏🏻🙏🏻🙏🏻🙏🏻

Can we use 100 microliter of bacteria solution instead of using swap??

can someone tell where TSA medium used?

What is that sample

THANK YOU MAM , BUT ONLY ONE WORD CAN CHANGED YOU CAN WRITE (DISK) THESE IS NOT RARE WORD THE WORD IS (DISC)

Plz remove background music

lol nothing about this video is sterile.

I still waiting for analysis video Of sugar percentage in any samples Please 🙏🏻🙏🏻🙏🏻🙏🏻