Detection of Salmonella species in Different Samples (Part-1)_A Complete Procedure (ISO 6579-1:2017)

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Detection of Salmonella species in Different Samples_A Complete Procedure (ISO 6579-1:2017)
Detection of Salmonella spp. is a very important microbiological testing parameter for Food, Feed, Water and Environmental samples analysis. This video represents a detailed procedure of for the detection of Salmonella in a sample. __________________________________________________________________________________________
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Video Links:
Detection of Salmonella (Part-2): • Biochemical Tests for ...
Streaking Method: • Streak Plate Technique...
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Related Tag:
#Salmonella
#Salmonellatyphi
#microbiology

Опубликовано:

28 июн 2024

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Комментарии : 118

@lithujoseph3474 2 года назад

Thank you for uploading this video.. and it's really helpful and understandable too.. please do video for detection of staphylococcus spp and vibrio spp.

@MicroChemsExperiments 2 года назад

Thank you. We will make video later.

@StephanieEatsss 3 года назад

Thank you so much, this video was very helpful

@MicroChemsExperiments 3 года назад

Thank you. Stay with us

@anormalperson1284 11 месяцев назад

Very on point and interesting video.

@MicroChemsExperiments 11 месяцев назад

Much appreciated

@Niyahgigs 3 года назад

Good job, I've got a cultured plate with distinct colonies and I'll need your help in identifying the bacterium and medium used. Thank you

@MicroChemsExperiments 3 года назад

Its a long task. Its difficult to explain here. Neverthless you can subculture your colonies on different selective & differential culture media like MSA, Cetrimide Agar, XLD, SS agar, Chromogenic Coliform Agar, MRS, MYP agar, etc. After that do biochemical tests of the selected colonies for identification.

@Djofficials 2 года назад

Thank u so much mam ...its very informative

@MicroChemsExperiments 2 года назад

You are welcome

@prasadch1368 2 года назад

Excellent explanation thanks 💐👍

@MicroChemsExperiments 2 года назад

Thank you

@isayasnemera4116 Месяц назад

Please,give us the link for part 2 and do the video for bacillus cereus

@simranpal452 Месяц назад

We have to take pre-incubated plates pf XLD or BSA?

@user-nf4oj8sy8b 2 месяца назад

Thank you so much. I try to find part 2 but cannot find. Please..

@waleed2980 3 года назад

Very info .thanks

@MicroChemsExperiments 3 года назад

It's pleasure.

@khushichaturvedi3817 Год назад

Tomorrow i wll face auditor in micro lab its very helpful to me thnkyo so muchh

@MicroChemsExperiments Год назад

All the bestttt

@khushichaturvedi3817 Год назад

@@MicroChemsExperiments i will try to my best

@bokulhossain5771 3 года назад

lot of thanks

@MicroChemsExperiments 3 года назад

It's our pleasure...

@hiwa_jalal 3 года назад

thank a lot 😍

@MicroChemsExperiments 3 года назад

Most welome

@hiruchaimae1604 3 года назад

Thanks for this video ! I couldn't find Part 2, are you going to upload it ? Thanks !

@MicroChemsExperiments 3 года назад

Already uploaded. Link: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-afpSDDvdGMc.html

@SirajulIslam-vp9gp 3 года назад

Thank you....

@MicroChemsExperiments 3 года назад

You're welcome!

@RahulKumar-nu1vf 3 года назад

Thanks a lot make more videos for E. Coli, and zeldahl test protein testing and show serial dilution also

@MicroChemsExperiments 3 года назад

Okay. We will make it. Stay with us.

@arunarao972 3 года назад

This vedio help me alot

@MicroChemsExperiments 3 года назад

Thank you. Stay with us.

@swuoj3893 Год назад

Thank u so much❤

@MicroChemsExperiments Год назад

You're welcome 😊

@sharonmamabolo9258 3 года назад

Very informative. Can I use distilled instead of peptone water?

@MicroChemsExperiments 3 года назад

No. Peptone water is used for Non-selective enrichment. Non-selective enrichment can not be achieved by distilled water. So you must use Peptone Water.

@dadaprincez7996 2 года назад

Thank youuuu

@MicroChemsExperiments 2 года назад

My pleasure

@kathrinajoycemuncal9074 Год назад

Hi! I just wanna ask if it is necessary to use both RVS and MKTTn for selective enrichment? as well as XLD and BSA for Isolation? thank you so much

@MicroChemsExperiments Год назад

Yes. Both needed for selective broth. And you can use only XLD for isolation

@BappaNondi 3 года назад

Waiting for part 2.

@MicroChemsExperiments 3 года назад

We will upload soon

@reddyyesubabu1327 2 года назад

Good job

@MicroChemsExperiments 2 года назад

Thanks

@angelikaplacencia8023 2 года назад

Thanks! Is part 1 already uploaded?

@MicroChemsExperiments 2 года назад

Both parts are already uploaded.

@claudiaalcost4378 3 года назад

What are the advantages and disadvantages of this method as compared to others detection methods?

@MicroChemsExperiments 3 года назад

1. Advantage: This method involves double enrichment steps which increases detection possibility and facilitates to detect Salmonella more selectively, thus this is the more accurate method compared with other methods. 2. Disadvantage: MKTTN media, frequently, becomes not available in local market and there is no replacement media of it.

@rambabu7652 Год назад

Very useful

@MicroChemsExperiments Год назад

Thank you

@ramdewarmanjistha8122 Год назад

hi your video is very resourceful but can we use selenite broth instead of RVS and MKTTn broth? and also another question, what is the name of this method used? thank you

@MicroChemsExperiments Год назад

Yes you can. Method reference is mentioned in the video title

@benedictagifty6097 6 месяцев назад

Do you have video on testing of pseudomonas Aureginosal. Please share

@MicroChemsExperiments 3 месяца назад

We will make it soon

@olabisiolatundun3696 Год назад

Well done my teacher. Please can I use this method for pharmaceutical samples?

@MicroChemsExperiments Год назад

Yes. You can

@ishagoswami6182 2 месяца назад

Mam what sample do you use?

@arzoosundrani1415 3 года назад

Please make a video on Bacillus cereus as per ISO 7932:2004 and Staphylococci aureus as per ISO 6888-2:1999 If possible.

@MicroChemsExperiments 3 года назад

Ok we will work on it soon.

@gustavohenrique2614 2 года назад

Can you name simpler tests for detection of Salmonella with close accuracy?

@MicroChemsExperiments 2 года назад

No other simpler method to be known with close accuracy.

@SnoooooowMan 2 года назад

Do you have a video for preparing xld agar plate? Because xld agar can not autoclave

@MicroChemsExperiments 2 года назад

We will recheck

@SnoooooowMan Год назад

Do you have a xld agar plate preparing? I don't know why my xld agar always in a liquid

@fariyahnaseem5184 3 месяца назад

Don't cover the lid of plate until medium is solidified N make sure the agar is completely dissolved in Duran bottle while heating in microwave This can be achieved when u see the medium is boiling clearly i.e bubbles formation.

@ritikachauhanvlogs175 2 года назад

Ma'am plz make a video on yeast and mould determination

@MicroChemsExperiments 2 года назад

Noted.

@SnoooooowMan 2 года назад

With the exception of RVS/MKTT, any proposed salmonella enrichment broth? Thank you

@MicroChemsExperiments 2 года назад

Not at all

@abhijitmukherjee441 Год назад

Salanite broth

@SnoooooowMan 2 года назад

Can we substitute XLD with SS agar

@MicroChemsExperiments 2 года назад

Yes. You can.

@worlujoy8490 Год назад

Is it compulsory to use all the reagents mentioned?

@MicroChemsExperiments Год назад

Yes

@zarqaarshad411 2 года назад

Can I prepare buffer peptone water from peptone

@MicroChemsExperiments 2 года назад

You can prepare according to the composition of Buffered Peptone Water.

@bolarinwaobasoge2716 Год назад

Pls why did u use 1ml of peptone in mkTTn broth and 0.1ml in RVS broth

@MicroChemsExperiments Год назад

Method said so to do that for the selective enrichment of Salmonella spp.

@exclusiveshow9721 2 года назад

Wauu. This is really a good video. Can anyone share with me a full document where I can find medium types used to enumerate bacteria founds in food. Please help if possible. Have a nice weekend

@MicroChemsExperiments 2 года назад

We can't provide any document. We are sorry. Thanks for your appreciation

@fariyahnaseem5184 3 месяца назад

Plz read bam manual

@thanith6432 Год назад

Excuse me, may I ask? What type of another sample that is easy to detect salmonella ?

@MicroChemsExperiments Год назад

Test poultry feces, that has the high possibility to detect salmonella

@maandancel5223 Год назад

What sample did you use??

@MicroChemsExperiments Год назад

Food Sample

@rehanullahofficals718 2 года назад

Can please confirm 6:16 mints its 0.1 ml or 100ml its confusing

@MicroChemsExperiments 2 года назад

0.1ml (100 microliter)

@achbiotech890 3 месяца назад

Mam, what kind of sample you r using?

@MicroChemsExperiments 3 месяца назад

Animal feed sample

@StephanieEatsss 3 года назад

What was the sample used?

@MicroChemsExperiments 3 года назад

Animal feed

@NONAME-yv3lq Год назад

2:42 what sample is that??

@MicroChemsExperiments Год назад

food

@istiakmostofa7167 Год назад

Why does solid foods need 24hrs for incubation after homogenized with buffered peptone water???..Not only for salmonella detection but also for enumeration of TPC??

@MicroChemsExperiments Год назад

24 hours incubation in buffered peptone water is needed for the enrichment of salmonella. But this enrichment is not applicable for TABC or TPC

@istiakmostofa7167 Год назад

@@MicroChemsExperiments then what to do for TPC after homogenized with buffered peptone water???

@MicroChemsExperiments Год назад

@@istiakmostofa7167 ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-IlTnLEgivts.html