Total Coliform Count (TCC)_A Complete Procedure (ISO 4832:2006) 

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This overlay of media is needed to facilitate the growth of bacterial cell on the surface of the first layer of the media

Yes. You can. ×10 dilution is the minimum

Yes. You can count colonies manually.

BGLB broth is used along with Durham tube

E. coli gives Blue colour colonies on TBX agar medium. So, other gram negative bacteria may give yellow colour colony. So you have to go for the identification tests to identify that bacteria

Eelam Music Production, Its soo tough to explain here to solve your question. But I'm trying.... Situation-1: You got 60 CFU from 0.1 BGLB broth culture ****Ans to the first question: 1. To get 10CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 59ml normal saline. 2. To get 100CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 5ml normal saline. 3. To get 1000CFU/ml sample, you need to use lower dilution in the same way as done for 1 & 2. ****Ans to the second queation: A single colony contains millions of CFU. First dilute 1 colony in 100ml normal saline then culture 0.1ml from the diluted colony on nutrient agar. Count the CFU on nutrient agar plate. Now Calculate and dilute the normal saline as much as you need to find your desired bacterial concentration, on the basis of how much CFU you got on NA plate. If you dont get the expected result by following my guideline then try the bellow procedure: First culture a coliform bacteria in nitrient broth, centrifuge at 4000rpm for 5 min. Discard the upper broth layer, dilute the lower bacterial cell in normal saline, prepare McFarland 1.5 turbidity and match the turbidity of your bacterial cell using a spectrophotometer. Then dilute to desired concentration of bacterial cell as needed. Thank you

@@MicroChemsExperiments can I use the distilled water instead of saline for the serial dilution?

@@Emusic1621 using diatiiled water for dilution in microbiology is not a good practice. You should use normal saline.

In first question,for 1000 CFU/ml I need to put 0.1 ml of bglb into 0.5 ml of saline right,but that volume is not enough for me,so what can I do to increase that volume without changing the value(1000 CFU/ml)

In first question,for 1000 CFU/ml I need to put 0.1 ml of bglb into 0.5 ml of saline right,but that volume is not enough for me,so what can I do to increase that volume without changing the value(1000 CFU/ml)

For Total Coliform Count, don't go for the enrichment. Enrichment is only applicable for detection method.

Yes. Those are also coliform

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Can you replace Endo Agar with VRBLA ?? Thank you

@@shazekorcari4922 Which organisms grow on this VRBL

We do not provide any pdf

Yes. You can use

@@MicroChemsExperiments thank you

Normally we count only those who have a number between 15 and 300 so the one with 0 is not countable

The maximum permissible limit of MPN in drinking water is 2.2 per 100 ml of water, as recommended by WHO

Diluent for Dilution of sample

Yes. You can use

Mention all your dilutions. Which lowest dilution you used to inoculate? Mention also the count of each plate.

Yeah, there's a Iimitation in this procedure when it comes to detection threshold. The best result you'll be able to provide is

@@Azmo477 you are correct. Thanks for the explanation.

Yes

VRBL agar medium is more specific for coliform

Pooja Bondre, Noted. We will work on it soon. Stay with us.

@@MicroChemsExperiments okk...

Yes. You can

To confirm isolated coliforms

Detection Limit is 1 CFU/ml

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10 ml

Thank you very much. Unfortunately at this moment we can't make videos in French. You can use translator or select french subtitles while playing the video

The maximum permissible limit of MPN in drinking water is 2.2 per 100 ml of water, as recommended by WHO

Your question is not clear

The ISO method said so

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Yes. The same method will be applicable for any kinds of food sample

Sorry I didn't get your request. plzz clarify

Water analysis test

@@safaafrin5391 please mention test parameter.

@@MicroChemsExperiments like aerobic colony count, listeria sp, clostridium perfringens, etc..

@@safaafrin5391 noted. We will make video for it. Stay with us.

Deep Pandit, Noted. We will work on it. Stay with us.

It is difficult to say because bacterial load depends on sample type and the environment. You will find large number of bacteria in spoiled food sample and in waste water sample

What if you serial diluted a water sample and in 1:10 you have 63 counted colonies and its number of bacteria per ml is 630, and you also have 1 colony in 1:1000 and its number of bacteria per ml is 1000. What dilution has the highest number of bacteria?

@@glacymaejacinto9451 The plate with 1 CFU should be considered in the colony counting. Count the plates with 10-150CFU per plate for coliform and calculate the total CFU of your original sample.

@@MicroChemsExperiments thank you for the info! 🤍

@@glacymaejacinto9451 you are welcome

Coliform must produce gas in durham tube. If gas production is failed the interpret the result as the count is less than 1 CFU/ml

Enumeration of coliform from food and feed samples.

@@MicroChemsExperiments this is method not for water??!!

@@aak-lm5ln Yes. This method is also available for water samples

@@MicroChemsExperiments but it is Approvalin food.

@@MicroChemsExperiments is there any method to confirm coliform in water with only petri dishes without tubes?

Yes.

Nop

Total coliform count of ice cream is same as water. If you want other parameter test for ice cream, please mention that parameter. Thank you

Salmonella spp. Staphylococcus spp. Pseudomonas spp.

@@bokulhossain5771 Noted. Hopefully we will start working with your mentioned parameter soon.

@@MicroChemsExperiments espectacular :)

@@migueth2787 Thank you. Be with us

Sorry, we use international language.

Artwork Shalini, I'm not good in English and I'm sorry for that. Please mute the audio and take help from the videos and subtitles.

@@MicroChemsExperiments Oh sorry...I didn't mean to hurt you... Anyways, the video was good. The important thing is that it was informative.

@@MicroChemsExperiments and I wanted to ask, what is MPN/100ml...I mean what is the full form of MPN?

@@ArtworkShalini No no I didn't mind for your comment. Your findings will help me to do better in future. I will try to improve my accent in future. Thanks for the comment.

@@ArtworkShalini MPN is Most Probable Number. MPN/100ml means Most Probable Number of microorganisms in 100ml of sample

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