This method is comparable to what can be described in the article referenced below. Water agar appears to be a great alternative for laboratory filter paper. www.ncbi.nlm.nih.gov/pmc/arti...
Just water and agar. Regular recipe, just no malt extarct or whatever nutritional source you might use would be. It allows the original transfer to be the nutrient source and for the mycelium to expand into the water agar that contains no nutrients.
Soooooo, have you changed your mind bout spending the cash on those well colonized [old] plates? Would you order from them again an would you specify your growth preference? Cool video
Absolutely, you won't find fresher genetics of this quality beyond maybe mycelia or amycel, and those are international orders that are even more expensive. These are the only genetic vendors I'm aware of in the US utilizing cryogenic. And this is very important in commercial production. What do you mean growth preference? I use 20g extra light malt extract 20g agar per 1000ml of water. Colonize at 70 degrees F. Backup on water agar in sterile distilled water. Thank you friend.
@@mycomunitymushrooms I'm enjoying your channel, I'm kinda new to things. I just meant in the video you were kinda humming & hawing (weird expression, I'm in NY so it might be an east coast thing) but how advanced the plate growth was.... so I was wondering that the next time you ordered you was like "yo brah, don't give me none them overgrown monster petri dishes". I don't know anything so ignore me, it's my boy an brother that were the growers, I'm just figuring it out. I'll keep watching.
@@fatkorn haha no you caught me. Why I mentioned it was because, the reason your paying for their cultures, in my opinion, is because they're at their youngest state from the clone culture they were taken from. So when I pay 100 bucks for a plate I'm hoping it's at its youngest state, and a over grown plate suggest it may have been in registration longer than my liking. Ideally it would have been just a week or two out of cryo, never refrigerated after take out of cryo. But I wouldn't expect them to make every order necessary like that... But more than anything I'm suggesting that even vendors working with cryo, I hope they ouldnt cut corners by just making a bunch of plates and keeping them refrigerated until orders come months out, as that would defeat the purpose. It can get a little complex, esspecially if your new. But all these commercial cultures are specific strains, that over time lose their vigour at least.
really enjoying your content. I think you would benefit immensely from hiring someone to help edit your videos a little bit! the music is a bit repetitive also. appreciate you sharing your flow and ideas. love what you do! also it appears to be metabolites in my eyes
Ya I have considered bringing someone on as a volunteer. I'm saving all the files so I can rerelease some even. I'm just trying to push out what seems most essential at first. Still have like 10 more I really want to get out. Thank you for the feedback!
I don't understand why you are paying so much for these plates instead of buying liquid culture and growing out your own. You can get really good genetics and avoid contam using your own plates. Why pay twice as much for a disposable petri dish?
Awesome stuff! Thanks for putting out these videos. I just stumbled upon them today. I've also been looking into long term storage and found a very interesting paper as well. Id love to know what you think about it as it covers a couple of different storage methods. One that interested me involved mineral oil and was said to be viable 3-8 years depending on temperature stored at. It does require an additional transfer at the end to bring it back, but seems to be an easy way to store some mycelium for a long time. This particular one (water agar) seems to be very nice if you need something
Very interesting! Saved! Thank you. I believe this could maybe have benefits because of the lack of oxygen. I bet we see more info on this approach in the future. May have to see if there are other studies like this.
