Long-term fungal culture storage in water vials. 

It was super epic the way you lit that burner! I don't know why that stood out to me so much but it looked like it was from like an action movie.

Hi! I wanted to rewatch your tutorial on long term storage and was pleasantly surprised to find you've got multiple videos on the subject! Thanks again for all the useful info. I followed your tutorial a few months ago, and was wondering if you've got a recommendation for how long I should wait to test the samples for viability so I know I didn't screw up the whole lot. I also noticed that you said the goal is to create an anerobic environment inside the jar so was wondering if filling the jar with more water would help to achieve this? With more water the samples would also float closer to the opening and (I suspect) it might be easier to retrieve them without contaminating the jar. Would you recommend using more water? Incidentally, I have a few old LC jars that I haven't touched in months that have a thick layer of mycelium floating on the liquid. Could jars like these be considered similar to water vials? There is plenty of liquid in the jars and I suspect the water has little of the original sugars left. Finally, what would you say longest a water-stored sample could remain viable? Thanks again for all your help!

Oh awesome here’s what I’m trying to do! I just have to get some bottles!

5:04

This might b a dumb question sry in advance lol. I use blue food coloring an it changed the water in vile bule is that a problem. Ps. Thank you for all the information love your vids

amazing video!

Do you think a spore syringe would survive after a half decade? I’ve heard spores germinate in the syringe, that would be almost the same thing minus agar nutrients?

Love the content! 🙏🙂

You could do a voiceover in order to film while the flow hood is on.

this video is gold :) Thank you. now I'm your psycho fan...

Great video!

What's that wrap you're using? Is it better than parafilm and cheaper? Can you please let me know what it's called or where I can buy it from? Thank you in advance 😊

Fascinating. I've never seen that before.

Wow ..awsome 👌 video

digging your videos man, good stuff! I try to comment on all of them for the algorithm, to help get them in front of more 👀

Thanks Ed, love you 😁

Great! keep em coming!

Will this work for any agar plate mushroom for long term storage? Great videos

Not sure if you mentioned it…is it recommended to put the culture to water agar and then to the sterile water agar? Or would it be better to use put an agar slice with a little nutrition in it?

is it possible to use sealed plastic water bottles? like ones sold in the stores

all that "auger", Youre killin me smalls 🤣

That seems like a simple method to do. Thanks. I did have a question. Like when we AI heifers on the farm we just have a select sires guy show up which his tank carrying semen we’ve bought and he can store that stuff for years. Do mushroom farmers ever store mushrooms long term in a tank of liquid nitrogen I believe it is. It’s kind of late or way early. Ya cause some bull semen I remember reading have been kept for decades. If mushrooms degrade over time after many propagations you’d think growers would be like storing cultures frozen in a tank so they’d really last.

Hi Edward, very live and educational video. Since you have a science background, how about glycerol stock of spores rather than water and keeping in regular freezer? This is just as a backup for purchased culture and for selective breeding (taking the biggest mushroom and saving its genetics). Like you, I have sterile bench experience, and I would hate to buy again a culture that I bought in the past and lost it due to contamination, drying out or flooded by condensation, labels coming off when spraying alcohol :), etc... My OCDs whisper to have a backup parachute... A dormant frozen glycerin stock was golden standard in bacterial cultures not sure fungi are the same. Like subscribed, thanks for sharing effective tricks that are not kicking science in the nuts. Cheers from Canada.

How many time one of those last viable?