Benedict's Test - This is the video on benedict's test which done for the detection of reducing sugars present in the urine.
Video contains Principle of Benedict's Test, Procedure, Discussion of viva questions and Demonstration of benedict's test.
Reducing Sugars - 00:28 minutes
Benedict's Test Principle - 00:52 minutes
Benedict's Test for Glucose, Sucrose and Fructose - 01:49 minutes
Color changes seen in benedict's test - 02:13 minutes
Composition of benedict's reagent - 02:38 minutes
Benedict's test procedure - 03:06 minutes
Benedict's test is a semi quantitative test - 03:27 minutes
False positive benedict's test - 04:55 minutes
Demonstartion of benedict's test - 05:33 minutes
All reducing sugars give positive benedict’s test.
Principle - Reducing sugars under hot alkaline conditions tautomerize and form enediols which are powerful reducing agents.
They reduce the cupric ions of Benedict’s reagent to red cuprous oxide.
Enediol is the enol form of sugar because two OH groups are attached to the double bonded carbon
Color Changes from Blue to Green to Yellow to Orange to brick red if there is presence of reducing Sugar. Sucrose will give negative benedict’s test
Benedict’s qualitative reagent contains:
1. Copper sulfate: Furnishes cupric ions (Cu++) in solution.
2. Sodium carbonate: Provides alkaline medium.
3. Sodium citrate: It acts as a preservative.
Procedure -
5 ml of Benedict’s reagent + 5-8 drops of solution
Apply heat to it for 1-2 minutes and observe
the color changes
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18 сен 2024
