Full description of how to measure protein CD spectra and perform thermal melts: Startup: 0:38 Setting up & taking a spectrum: 1:34 Analyzing %Helical Content: 7:56 Preparing a thermal melt: 11:37 Analyzing Tm: 13:41 Shutdown: 16:04
your video on this instrument is very helpful for me. i want to ask you one thing, what is the formula of this instrument to calculate the difference in absorbance which is plotted here as you shown? difference in absorbance = Absorbance of left circularly polarised light - Absorbance of right circularly polarised light or may be opposite of that.
The operation protocol is clearly understood except for variable temperature. I want to measure a CD spectra for my sample whose absorption is 350nm and I could see a bisignate cotton effect. I want to measure temperature dependent CD from 20 to 90oC using JASCO J 815 please help with notes or vedio manual. thanks in advance
Here is a webinar that should help: jascoinc.com/learning-center/webinars/applications-in-fluorescence-spectroscopy-webinar/ Also several videos that cover software details including temperature ramping: jascoinc.com/training-video/video-category/spectra-manager-for-cd/
