Thanks a lot ma'am. I hope you would also consider demonstrating how is antimicrobial testing being done for extracts derived from plants or animals. Thank you
Ma'am gusto ko sana malaman kung pano gawin yung broth culture at home. I researched na need pa ng autoclave. Is it possible na may alternative way po to autoclave it? I need this po kasi to make my research feasible.
Broth culture medium can be done similarly minus the gelatin as solidifying agent. Alternatively, you can boil meat preferrably beef to get extract for better medium. You may use pressure cooker to replace autoclave.
Pwede naman buksan pero dapat sa laminar flow or Basta sterile environment para hindi macontaminate. Tapos dapat may gloves at mask kasi baka pathogenic Yan.. pwede naman iprolong ang incubation para lumaki colonies..
As a hypothetical: What if you were stranded on an island and had to make penicillin from scratch …? How would you make your medium (if there were no Supermarkets)…?? THAT would be an interesting video….if you were rebuilding civilisation and had to make penicillin from scratch, could you?
Interesting. It would probably be possible considering that I know what to do, but it will take ages in the absence of materials and equipment. I would probably go for herbal plants as a source of antibiotic..
@@jhonamiemabuhay1886 Herbal salves/compresses would work for local infection - I've read that certain tribes used certain mosses and raw honey to dress wounds but as a treatment against something such as sepsis where the infection is throughout the body, would Herbal remedies be effect. Even Penicillin today has become ineffective against many infections. ...Also if herbal remedies WERE THAT effective you would think that they would already have been widely used during the many wars preceding the discovery of Penicillin in 1928 and it would have been redundant, right?
Hi ma'am, thank you for this video. May I ask po what contaminating action did you do on the sample E (the one in the jar lid) for it to have that visible colonies compared to samples A to D? Thank you.
Thanks alot ma'am! I've been searching procedures to do a bacterial culture at home for months now! this helps alot on our research project Thank you po!
Maam may I ask po if we can use an Egg incubator to incubate the bacterial culture? the incubator also runs on 35-37C but not that stable po since bumababa and tumataas, can we use it po as a substitute incubator
Good day po maam, bakit po pala bawal siya hawakan? so bawal mo po siya itransfer to any part of your body? And moreover po, when it comes to culturing good bacteria, same procedure lang po ba as shown in the video?
We are not sure kasi if what we are growing is bad or good bacteria. No way to tell. Mas okay mag-ingat. Same procedure lang although cannot separate bad from good bacteria kasi general culture medium sya...
thank you so much po I'm really using this for our home based research testing, but i have 1 question po, how do you dispose the agar plates properly??
@@jhonamiemabuhay1886 oooh thank you po, ito nalang po yung need ko isagot sa research adviser namin, tysm po naperfect ko po yung performance task ko.
If used at home pwede na rin boiling water lang. If you have uv light you can use that too. In the lab, plastic petri dishes are disposables. You can buy it sterile then use it only once. It cannot withstand autoclaving.
Good day po, Ma'am. Can I ask if by using this way of culturing bacteria will it be possible to grow Propionibacterium Acne Bacteria? And if yes, how to do it po? I'm a SHS STEM student and I need the bacteria for my research po.
Isolating bacteria needs selective culture media. It is possible to grow bacteria from acne but to determine which one is your target is very difficult to do
Difficult to do it at home. You will need selective culture media for isolation. If allowede kayo gumamit ng lab, doable naman sya sa lab. You can use culture media like EMB agar, TSB or BHI
@@jhonamiemabuhay1886 okay po, Ma'am. Thank you so much for answering, this is a great help since I've been struggling with this po. I'll just edit my paper and do what you did. I'll also give credits to you po. Thank you so much ulit
Medyo mahirap po yan sa characterization ng colonies. You poured your culture medium too early. Kung sobrang mainit pa sya magiging wet talaga yan. Wait ka until medyo warm na lang ang medium before mo ipour sa plates.
Hello po Ma'am! We are tasked po kasi to take picture po after 2 days, 4 days and 7 days po tapos I may have pour the gelatin too early sa mold and as a result, the cover is slightly burry. Is it okay po to take picture without the lid off during those days? Or is it very dangerous to do so?
It is dangerous. You can devise a contained environment before opening it. Wipe the surface of the lid then put back before taking photo. Opening it without containment would mean contamination.
@@jhonamiemabuhay1886 additional question maam hehe, is it ok if i'll put the whole bouillon beef cubes? im afraid baka mafail yung homemade agar ko po.. and what's the proper incubation po? thank u maam!!
@@baemax5769 hi. If you will use the whole cube it will darken na color of your medium. It will be difficult to see other colonies and maybe too much na ang trace elements like salt that may inhibit growth. Incubate at room temp not higher than 35 degrees para hindi magliquify
The laboratory grade nutrient agar usually contains peptone, beef extract, and agar primarily as solidifying agent. In addition to these nutrients, some vitamins and some trace ingredients necessary for the growth of bacteria are also added. The peptone is the source of nitrogen or protein. The beef extract is the primary source of carbon which is essential for the formation of carbohydrates in the bacteria. It also contains other components like some vitamins, different trace minerals, organic compounds, and salts, which further enhance the growth of different organisms. Because I was only trying to make use of what is available at home, in the kitchen, I thought of using gelatin as it called (but really agar as they are made from 100% algae extract) to be the solidifying ingredient. I used beef cubes which is a replacement for two ingredients, the peptone and beef extract including different trace minerals, organic compounds, and salts for the purpose mentioned above. Protein is very important in culture media because it acts as a source of amino acids for the bacteria, necessary for gene expression.
Gelatinase break down peptide bonds, which is present in protein-materials like the broth cube. But, agar is a carbohydrate, it is not a protein. How does the gelatinase produced by the bacteria degrade the culture medium? Is it because the broth cube/protein have been mixed well together with the agar solution that breaking peptide bonds also physically damage the agar? Thanks.
In my humble experience in laboratory, very few bacteria can degrade agar because it is cellulosic, made from algae extract. Gelatinase can work usually on gelatin made from animal extract which is usually 98-99% protein so you are right that it breaks down peptide bonds so it can solubilize gelatin.
Hi po ma'am! isa akong farmer rice specialized nag reresearch ako ng mga microorganisms na makakatulong sa organic farming ko po. Nag aaral din ako kng ano ba talaga Ang kilangan ng bacteria and fungi to grow properly. At Ang isa Kong nalaman ay sa pamamagitan ng protein. Sana po meron akong maraming makuhang idea para makatulong sa pagsasaka.😊
@@jhonamiemabuhay1886 ma'am bakit ? Molasses Ang nilalagay na pagkain ng microbes po? Kc Ang sugar kapag subra Ang baba ng pH level nito. Meron po bang alternative para sa sugar or molasses? Ang ginagamit ko po kc ay kunting sugar lng mas marami Ang salt. Saging na hinog at kamote. Hnd po ba bumababa Ang pH ng lupa kapag molasses?
@@lazygardeneasylife2774 Okay naman ang molasses. Kelangan lang tama ang amount na maconsume sya ng microorganisms para walang excess. Marami sa microbes, esp fungi need ample amount of sugar for metabolism. They thrive better in slightly acidic environment. Magandang experiment yan kung gusto mong gawin. pwede mo imonitor ang pH para makuha mo tamong amount ng ammendments mo
Ok po salamat po ma'am 😁 karagdagan lang po na tanong. Nabubuhay po ba Ang fungi sa anaerobic environment? Para kasing kadalasan nasa topsoil lng Ang fungi po. Sana masagot🙏 Para magkaroon po ako ng better understanding 🙏🙏🙏❤️❤️❤️
@@lazygardeneasylife2774 meron din fungi sa sub soils or kahit sa medyo malalim pa na area. Meron pa rin amount ng oxygen. Pwede ang fungi sa anaerobic envi kung spores sila.
Hlo madam can we prepare culture media for bacteria using natural ingredients plzz reply I'm doing project on it and suggest some natural ingredients plzz 🙏
Yes you can. You can find something like seaweed as solidifying ingredients, boil meat like chicken and beef as source of protein. You may add natural extracts.
It is better to use plain and unflavored coz flavoring might disrupt growth of microbes but if you want to try, why not. Then pls let me know the results. Microorganisms can grow just about anywhere...
I've tried before po using agar agar powder. Then overnight ko po sya nilagay sa ref, nagliquify pp agad sya 5 minutes after ilabas ref. Bakit po kaya?
@@suialekzandriamhai3328 try mo idouble concentration. Make sure natunaw ng husto ang powder. Kapag hindi sya natunaw, mabilis yan magliquify. Sa video, ang ginawa ko triple dose un...