DIY Tissue Culture: How to Get Started for Less Than $200

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I sacrificed a finger to bring you this video. please subscribe
Join the Plants in Jars discord server! / discord
Where I buy tissue culture supplies: www.plantcelltechnology.com/?...
(use code PLANTSINJARS for 10% off)
/ plantsinjars_tc
Supplies List Below!
• Scale (0.01g accuracy): amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): amzn.to/41CqJFA
• pH Up & Down: amzn.to/3pAM19r
• Spray Bottle: amzn.to/3pI3Q6g
• Plastic Gloves: amzn.to/41CBBmW
• Forceps: amzn.to/42K3VFa
• Scissors: amzn.to/3I9dwgK
• Sterile Petri Dishes: amzn.to/3nPNHLT
• mL Droppers: amzn.to/44X8cXl
• Agar: bit.ly/3o4yPcu
• Murashige & Skoog: bit.ly/3pMpAOr
• Kinetin: bit.ly/41IpXaa
• Polypropylene Containers: bit.ly/3pJrlMF
• Light Meter: amzn.to/42B1THI
Protocol for video: www.researchgate.net/publicat...
(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)
Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.

Опубликовано:

13 май 2023

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Комментарии : 1,2 тыс.

@jorgesantiago521 Год назад

I do this professionally in a research lab. I’m honestly impressed by your work. Great job and keep it going!

@plantsinjars Год назад

I really appreciate that! Thank you

@dudeleboski2692 Год назад

Great clear and easy to understand Video😊

@Izzy-fr1zu Год назад

Can you answer a question that was popping up in my mind, when watching this? The process doesn't look too different from regular plant propagation with cuttings. Wouldn't this techniqually also work without the hormones? My guess is, that the hormones just speed up the process?

@chriskennedy7534 Год назад

@Izzy-fr1zu Was thinking the same, it was curiosity for difficult to strike plants that led me here

@plantsinjars Год назад

@@Izzy-fr1zu Yes, there are a lot of plants you can tissue culture without plant growth regulators. PGR’s can speed up multiplication and rooting, but sometimes (if used in too high of quantities) they can stunt plant growth

@juandiegosoledadbarajas Год назад

I am a biotechnology engineer and it is wonderful to see this kind of micropropagation content! Let's hope that more people get their attention to this beautiful thing about science!

@noobcup 2 месяца назад

I had never heard of this until I came across this video. Now I’m freaking hooked

@ShineAqua Год назад

I got an associates degree in horticulture, but couldn't justify travelling to the land grant school for a Bachelor's, so as a certified plant nerd, this is the raddest thing ever. I remember buying a pineapple plant propagated this way from Disney's The Land ride, it made me want to study plants and hydroponics, and I love combining tech and nature. Keep this up, please, and inspire the next generation of plant nerds.

@Zayskibop Год назад

Mycologist here, been dying to learn plant culture, this is such an incredible series (especially when you have everything you need) 🔥❤️😂

@spackerinternational6131 Год назад

Hello fellow mycologist! At the start of the video she states that you can create millions and millions of plants this way. I know for a fact this is not possible in mycology as eventually genetic senescence creeps in after you've expanded mycelium X amount of times and you get diminished results and you have to start again from spores.. I'm curious if this is the case with plant cloning.

@Zayskibop Год назад

@@spackerinternational6131 what kind of fungi do you study as well?! 😌

@spackerinternational6131 Год назад

@@Zayskibop mostly? The type that bruises blue.

@rebeccaspratling2865 Год назад

@@spackerinternational6131 same. 😂

@sleepn_on_me2473 Год назад

@@spackerinternational6131it has to be. Thats why in general gene flow and gene mutation is good because you get an entirely new breed from an original population

@carolynconway3893 11 месяцев назад

It was actually great having your fiancee do it live, with your coaching and notes! I feel like you added a lot of really helpful details that someone with experience might otherwise forget.

@onepiece666 7 месяцев назад

I have worked many years in many labs doing this for strawberry and other species. The fact contamination is not a major issue for you is a miracle since for many people, working with wild plants, contamination is always a problem. Great job

@jonathanbennett1957 6 месяцев назад

I even added gloves to my still air box when I was working with liquid culture making Petri dishes growing mushrooms

@ultrasoft5555 Год назад

As a biologist, I find your work very-very impressive, the content you share here is amazing, I am stunned! And of course I've just subscribed to your channel :)

@PlantCellTechnology Год назад

Thank you for helping make tissue culture accessible!

@nee3029 Год назад

Your channel is the best on TC! 👍

@PlantCellTechnology Год назад

@@nee3029 Thanks for your support! And, you like our content.

@greensage395 Год назад

I am 60-year's old, and a Life-long plant Lover.....when I was around 13, I went into a Woolworth's that had a luncheon area/kitchen/diner. I found a Product Stand with Test-tubes containing clear gel and a small portion of plant growing in it. The Cardboard backing it was attached to advertised colorfully as "Grow anything in this medium", and I was captivated. To me it seemed like incredible Science to do such a thing, ...Cloning! I really wish I bought it, but knowing Regulations in those days, it was probably best I didn't considering the Ingredients you are talking about! :) I might of grown a few extra fingers, and then some! LOL

@cannabisscience8969 Год назад

Let's go! Great video series. In my plant pathology grad student years, I did tissue culturing to remove viruses from fruits and vegetables in a lab with probably $50k+ gear. Getting this down to $200 to bring the science to the masses is just awesome!

@2010dragonclaw Год назад

I'm sorry you got injured! I'm a surgeon and i remember the early days of putting blades on scalpel handles in class. Blades were flying everywhere!(especially with brand new handles, they were very difficult) 😂. Nowadays, nurses put them on for me in the OR or we use disposable scalpels in the clinic. Hope you are healing well, glad to see it didn't stop you for long. Keep up the good work! Thanks for sharing.

@jenny-DD 6 месяцев назад

She's already got a man 😂

@darreneast4443 Год назад

One of the best tutorials I've seen, regardless of subject. No relevant info left out, and only relevant info kept in. Both concise and complete. Thank you for taking the time to both create and share this, it's very much appreciated. Oh, and all the best for your approaching nuptials. May you both have a long and happy life together. Cheers!

@awnage Год назад

26:06 "Plants are Science". This reminded me so much of one of my grad school classes from 20 years ago. Well done making it accessible to the world.

@OrderUpASeven12 8 месяцев назад

I know y are too young to have seen a movie called "Green Fingers" But when I saw your arm and of course the subject matter of this video; I thought about that movie. Good stuff, I used to do a lot of micro for work and one particular company refused to buy a laminar flow because they6 said it was too expensive, so I was forced to do all the culturing in a small closed room just using basic aseptic techniques. Despite losing many cultures (fungi) to other non specific ones, the usual culprits, we did indeed succeed with "jam jars" and no "proper equipment" LOL.

@debbiefox6846 Год назад

Omgosh!! I am so excited your video showed up in my feed. I love plants and own about 140 houseplants. I love studying botany & science. You really broke this down into such an easy to follow tutorial. Loved this! I have always wanted to do this with orchids. I'd probably also do this with all my Orchids, Hoya, Dischidia, some ferns, Jungle cactus, Zig Zag Cactus and my philodendrons. Thank you for sharing and I am honestly so sorry you got injured badly. A 3 day hospital & severed tendon are a huge deal. I hope you don't have nerve damage & your rehab goes well. So thank you for your sacrifice for Science. I can't wait to binge your other videos abd my inner nerd is super stoked! Take care & get well soon.🫶💚🪴☘️🌵🌿🌱🍃

@plantsinjars Год назад

Thank you! I have a video on my channel about cloning orchids from stem propagations if that helps! It's an older vid so the quality isn't as good but the info still holds up. I am also an orchid lover :) Someone gave me a great idea this morning to make a video about growing orchid seeds in vitro.

@ripoffrecords Год назад

Nodes are generally the best plant material because they contain an accumulation of meristematic cells ~ the 'building blocks!'. I'm an arborist but I grow everything including mushroom and fern spore cultures. I just started tissue culturing birds nest fern prothalli because I need them to grow and multiple much faster. This was helpful thank you.

@TiberiusCowgill Год назад

This popped up in my recommended feed, so glad it did. This content is truly amazing, and I'm shocked you haven't hit 100k let alone 10k subs yet. Keep up the great work, truly amazing and educational!

@LaurenJacksonlocs29 Год назад

You can also use baking soda to up the PH and distilled white vinegar to lower it to save some extra bucks 😊 I love your videos! Thank you for sharing thorough easy to follow info on PTC✌🏽🌱✨

@plantsinjars Год назад

I love this tip! I wish I had mentioned it in the video

@nolongerabandoned Год назад

@@plantsinjars baking soda adds alkalinity but not pH. You could use a diluted NaOH (lye) or liquid calcium hydroxide. You did an excellent job on this video! I am looking at growing giant bamboo here in the Philippines commercially, and your video is the best explainer I have seen yet. Thank you!

@TheDuckofDoom. Год назад

Vinegar is a very poor choice it has chemical similarity to the auxins. Normally phosphoric, sulfuric, or nitric acid would be good choices in hydroponics as they are all plant nutrients, citric acid may also be good and does not effect the nutrient balance(food grade is often found with canning supplies at low cost). Phos is the most common hydroponic pH down. Non-adultrerated ammonia is a fair choice for pH up (it will convert to more stable ammonium as it grabs protons) but KOH or potassium carbonate are also good (not bicarb. aka hydrogen carbonate). Most other bases are either poorly soluble or will interfere with the biochemistry; sodium is generally tolerable in very small amounts though a few species are quite sodium tolerant. Nitric acid is a tricky one for normal hydroponics with large active plant growth, plants love to absorb both -NO3 anions and +NH4 cations in much larger quantities than other nutriants. The plants exchange -OH ions for nutrient anios which raises the pH and with nitric you can end up chasing the pH if not aware of the bigger picture. Likewise ammonium cations +NH4 get exchanged for +protons and this lowers the pH. So the two must be balanced, and that balance varies with plant specie. The situation is slightly different with tissue culture because of limited nutrient uptake, and the media is a gel so the bulk solution cannot readilly buffer local changes in pH.

@LaurenJacksonlocs29 Год назад

@@TheDuckofDoom. wow, lots of info! But the whole reason I mentioned vinegar was the very affordable cost lmao because that was the point of the video? Yes, if you have a bigger budget and access, of course get the best of the best. All I know is vinegar is like 3 bucks for a gallon 😂 and my cultures have turned out great if I even had to use vinegar. As you know, before adjusting PH, it tends to be in the low 4 range. Vinegar isn’t needed unless someone went too far over, which is less likely. Btw, back on topic of budget, how much do the options you mentioned cost?

@LaurenJacksonlocs29 Год назад

@@nolongerabandoned it literally changes the PH. Have you tried it? The meter don’t lie 😂 But baking soda is a great option for someone starting out on a budget. And if you do need to use it, you use a pinch of it. It doesn’t take much at all to raise the PH.

@IronAlien Год назад

Good on you for not giving up.

@Lemurai Год назад

This is actually great, when I went through my biochem major, part of our senior project was industrial produce research & production then up scaling the process. We even did plasmid insertions for specific trait expressions. It was good stuff, we got to work with the Chemical Engineering class in teams to scale the process. I like things like this, it’s good to see younger people on RU-vid doing productive things.

@SansNeural Год назад

One thing that struck me here is the quality of the comments... says a lot about the quality of her content!

@thehuntfortruth Год назад

I looked into this years ago because I wanted to do this and it looks like now the technology finally exists!

@plantsinjars Год назад

The technology has existed since the mid-1900’s- it just hasn’t been very accessible! Hoping to change that

@user-od7iq9kf6s Год назад

Thanks, I have propagated plants for years. This is WAAAAY beyond anything I have ever considered! Ver exciting!!!!!

@tenekevi Год назад

Really nice explanation. BTW, scalpel blades can be inserted manually, you just have to know the technique. During surgery, we place blades manually but remove used blades with a tool. Only to avoid slicing ourselves with a used blade.

@logospathos3202 Год назад

I am a lover and a collector of rare succulents and this is a cloning technique that interests me a lot. In recent years I have collected a lot of information but your video is a perfect synthesis of everything that is needed to carry out this project. Great job and thank you so much! 🙂👍 P.s. I subscribed to your channel even before seeing that the channel is all about this technique! Fabulous! Bravissima! 👍👍👍

@jenny-DD 6 месяцев назад

I'm a lover too ❤

@jpdel4391 Год назад

I can't believe how much this taught me, great content and super informative! Thank you so much.

@gabrielhamilton2880 Год назад

You give off big Mad Plant Scientist energy. Subscribed.

@IntenseVisuals Год назад

This has to be the most interesting topic and video I've been recommended in a long time.

@loicpage5640 Год назад

Congrats on the views for this video! Your passion and hard work is very inspiring . Keep up the great content.

@squishy_madness Год назад

thank you so much for this video! it was the best video i have seen so far on getting started in tissue culture. i appreciate you considering budget, depth of detail, showing the procedures, and for making it seem so approachable!

@navturn Год назад

I had no idea this was possible. So amazing !

@Bondismo Год назад

This was fascinating! Thanks for showing that the barrier to entry is doable at home to this super cool technology

@timturk1899 Год назад

Great video! I've cloned plants, and always thought Tissue Culture was only possible through highly trained scientists in a sealed lab..?! You sure busted that myth, lol! IDT anyone could have broken it down into a more simple, understandable method. A new channel to subscribe to! Many thanks, and much respect!👍💯✌️🙏

@plantsinjars Год назад

Thank you!

@mahyarshokraeian Год назад

I'm so happy you finally made this video! It helped me a lot! 🤓🙏

@plantsinjars Год назад

Thank you! I'm glad it was helpful!

@strayargus9382 Год назад

I love this! Don’t think I will be doing tissue culture but I love watching the science behind it! Thank you so much, both!

@Highlander.7 Год назад

Well put together video!! Very concise. Blessings and may you recover quickly

@TheSkyHive Год назад

I found you today and I am happier for it. I have been growing mushrooms for 25 years and have always wanted to tissue culture. I have lab grade equipment thanks to my fungi obsession so I will only need to buy the media. I am ordering supplies from your links and getting started. Thanks for prodding me into action...PLUR

@thecloneguyz Год назад

We use UVC bulbs to STERILIZE our growrooms 5min intervals x 3 times per day LITERALLY KILLS ANYTHING ON CONTACT - Spores, bacteria, Insects. We use it as a PREVENTION TECHNIQUE NO CHEMICALS Make sure you're not in the room when the lights come on because it's highly cancerous to human skin but the plants love it and you can accidentally kill your mushrooms or your good bacteria with it if you're not careful

@bobnewkirk7003 Год назад

Fantastic Video, it was very informative and well put together. I'm a professional Microbiologist and can verify that your doing everything as well as you can given your limitations; still air boxes are great. I have long wanted to setup my own BSC but haven't dedicated the space or time to it yet. You may not want to introduce other contaminants into your space but this skill set translates almost 1-to-1 into other forms of Micro work. If you or Robert have any interest in home Fermentation or Mycology you could easily expand your setup to include them. Additionally, some of the ingredients you mentioned (like agar) may be available cheaper from a supplier outside of the dedicated tissue culture space.

@sleeplessinthecarolinas8118 Год назад

Thanks for creating this helpful video. I hope your recovery is swift and uneventful!

@lemonlizard1 Год назад

this is such a great video! Can't wait for part 2

@jeremiahbullfrog9288 Год назад

This is amazing, I have been doing amateur mycology for some 2 years, had no idea you could do this with plants. Thank you for sharing your science.

@sdqsdq6274 7 месяцев назад

? this has been done in the aquarium plants hobby for such a long time way before house plants

@jeremykenward3082 Год назад

As a plant person (mostly edible/medicinal) that also dabbles in mycology, this video is great! So much cross over skills/equipment/techniques. Never even considered tissue culture at home as a thing...but I just may start exploring that this year, or over the winter. I already have a big chunk of the stuff. Thanks so much for sharing this!

@working2bselfsufficient724 Год назад

I was thinking samething, same equipment used for growing mushrooms indoors.

@d.jensen5153 7 месяцев назад

@@working2bselfsufficient724 Yup! Back in the day I bought a HEPA filter that worked _so_ well at creating a sterile field for nutrient agar inoculation w/o contamination. I'm sorry I threw it away! I didn't imagine I'd be doing green tissue propagation in 2023, or that it would even be useful for such a thing.

@gurkengamingpvz21 Год назад

this will be needed by me in the next few years. thanks a lot

@Curixq Год назад

Yep, a new project to add to the list! Thanks :)

@hotdubtime 8 месяцев назад

People say without sterile air pressurization there's a risk of jar contamination. And that it's problematic to sterilize the medium without autoclave. Can we see the results?

@DustyQatun 7 месяцев назад

There is definitely an increased risk of contamination, but with proper sterile technique, we can lower out error margin to the point that it doesn't matter to non commercial people. It doesn't really matter to us if we lose 2-3out of ten jars (Not even that much once you get your technique practiced) but for someone making 1 million jars it really adds up

@neila.4289 5 месяцев назад

Pressure cooker can sub for autoclave…

@larkbox8427 Год назад

Hi, Great vid. Your clean box is very similar to the one i use for my brewing yeast culture play.. However i use a pane of glass on the top of my box instead of inverting it onto its lid, which provides much better vision, and also helps stop inadvertently breathing into the box when peering in through the arm holes tho that could be just me..

@spencerwilliams4734 11 месяцев назад

I love how you explain this I love plants 😮

@mikehammers4800 Год назад

Such a geek and LOVE it! Keep doing what you do!

@bkm8707 Год назад

And for your next video, can I suggest teaching us knife throwing? I kid, I kid! Excellent video, I really enjoyed watching it and learning about home tissue culture. The last segment was quite entertaining watching you try to teach us about aseptic technique while Robert fumbles around with his hands in a clear box, lost, but trying his best like any good man in a mall shopping experience. Kudos to you, Robert, for being supportive and a great sport about the task at hand!

@jeffravage3829 Год назад

Presterilized scalpel blades come in a foil pouch with a cardboard insert to allow attachment to the handle without removing the blade. Peel back the foil so the slotted shank is exposed. Grasp the pouch, with your gloved fingers tightly on the flat. This will allow you to insert the handle while the blade is secured, covered and still sterile. Dont ever take the blade out of the pouch without the handle attached. That way lies madness(or great ouchieness). Excellent video, easy for newbies to follow. Keep it up!

@nick42star 10 месяцев назад

Thank you, your attention to detail & simplified short cuts are truly inspiring along with your enthusiasm in doing something you enjoy, I grow a huge amount of perennial & annual garden plants & this year I bought in some tissue cultured delphinium & my knowledge in this is..& was limited but truly find your explanation a joy & considering setting up some basic laboratory myself. I currently have a ever growing collection let alone the ones I sell, it's such fun & I have so many happy plants & ever more happy customers... 11 years in & so different from my training in carpentry.. Keep up the good work & love your posts, thank you 🌻

@danielharrold1075 Год назад

Absolutely love your video good on you.. Great to teach people how to do basic TC...❤

@mikioashi3038 Год назад

Great video!! I started a tissue culture at home for 2-3 years. Here in Thailand, I'm trying to keep it low on the budget and spend less than 50$. I was scared of the surgery scalpel too. I using the X-acto knife, which uses the same style of blade but easier and safer to attach. Using a knife will make the wound on the ex-plant smooth and less susceptible to infection (from my experience). And I am afraid that the media will be contaminated so I heat them up in microwave and drop some bleach into each jar while still liquid and leave it till there is no smell of the bleach before using(for a couple of days), the result comes out great.

@thecloneguyz Год назад

I would be very careful with bleach since it's residual and it doesn't just wash away and it sticks around for years

@1DwtEaUn Год назад

As far as first plants to tissue culture strawberries because they are easier-ish and there are a few rarer varieties that I'd like to incorporate in breeding Premier aka Howard 17, and Catskill. Next would be peach, to preserve rarer types like Triogem.

@plantsinjars Год назад

I’ve been wanting to try strawberries! Let us know how it goes

@d.jensen5153 7 месяцев назад

This is gold! Exactly the info I was seeking delivered in a most pleasant way! Thank you, thank you, thank you!

@chrisb.5570 Год назад

What a great basics video! So sorry to hear about your finger. I'd like to try some chimera African violets since they can't be grown from leaves. I'm also interested in meristem culture to clear violets of INSV but that's a bit advanced for now.

@plantsinjars Год назад

Thank you! Clearing INSV through culturing meristem tc sounds extremely interested. Keep us updated on your progress!

@linzyweber7247 Год назад

Thanks for this! I have been reading a lot of books about getting started, and the visual representation is really helpful. Sorry to hear about your injury. Best of luck on healing.

@plantsinjars Год назад

Thank you! I’m glad you found it helpful

@gavinmiller3637 5 месяцев назад

Wow! I'm so glad this video just appeared on my recommendations. 🙏

@edgarsmittenheighnjenkson9226 Год назад

Definitely trying this.

@marcfruchtman9473 Год назад

A really great resource. I do however urge caution with regards to any 'math' done by LLM AI such as chatGPT... it is very prone to error, so just be careful, and for important calculations, double check the calculations (at least in 2023)... BTW, awesome info about the recycling symbol. Just discovered this channel [subscribed]!

@metatechhd Год назад

🔬🌱👏 Your professional work in a research lab is truly impressive! It's amazing to see your dedication and the results you've achieved. Keep up the great job and continue pushing the boundaries of your field. 🌟💪 Your passion and expertise are inspiring, and I look forward to seeing more of your groundbreaking work. 🚀🔬 Keep the innovation flowing!

@duleepgoonewardene Год назад

Greetings from Srilanka. Very practical and informative. " The couple that works together , stays together."

@paigemaloney3216 4 месяца назад

amazing explanations, thank you so much!

@NagashiChidorii Год назад

Wow smart and GORGEOUS TOO! I love how thorough and attentive you are in all aspects of doing tissue culture at home. I've already been doing it at home so its nice to see what I have figured out already and compare it to your suggestions. Amazing. and thanks for the tendon sacrifice for this video lol

@NagashiChidorii Год назад

Holy crap just finished the full video and you even give ChatGPT tips (which was a great idea!!) + have good edits like showing yourself crying and trolling yourself lol

@sparklessparkles728 Год назад

Great video! Plants are science but you make plants fun as well!

@plantsinjars Год назад

Thank you!!!

@pedrosanchez702 Год назад

Been waiting for someone to post this exact vid, thank you!!

@kopasdupas Год назад

This is awesome!

@joegurt4782 Год назад

:O ROBS IN THE VID!?!

@tim3780 Год назад

Actually planning to build a laminar flow hood, just will take a while to acquire all the fitting parts

@plantsinjars Год назад

That is awesome! Keep us updated on how it goes

@flapajawea Год назад

Gordotek has a great video on laminar flow hoods 👍 I build a similar one and it works great

@flapajawea Год назад

ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-lInfdAVvBts.html

@timothykramer1856 Год назад

I can second the GordoTek laminar hood easy to build and it’s working for my mycology. I also use a instant pot instead of a pressure cooker. thanks for starting me on a new hobby never thought of home tissue cultures. Thanks great video. ❤

@zeb33369 Год назад

I have never subbed faster, thanks for being a whistle blower on big tissue culture 😁

@imikla Год назад

Awesome video! You are really getting better at editing and creating with every video! Since I've done tissue culture many moons ago in a lab, things appear to have gotten a bit easier (we actually had to make our own MS Medium from scratch with the individual chemicals!). Now I'm working to get back into it in my home 'lab'. I have quite a few of the supplies, but I'm still not satisfied with any of the culture containers I can find. I do however... *really* like (dare I say ❤) those jars with the PP lids you are using. Can you please share where to get those?

@plantsinjars Год назад

Sure! They are the 240 mL culture vessels from Phytotech. Thanks for your comment! phytotechlab.com/culture-vessel-glass-70-mm-8-oz-240-ml.html

@imikla Год назад

@@plantsinjars Awesome! Thank you so much! And they have them with filter vent tops!

@danieljheelan5256 Год назад

Hi Laur, Thanks a lot for sharing. I tried several times but each time the cuttings got contaminated. You don't get problem when you leave your tools in bleach? After my TC, I wash all tools with water and wipe them but all my tools get corroded each time I use and sterilize in bleach. Working with anthuriums these days. Daniel Jheelan from France

@plantsinjars Год назад

@@danieljheelan5256 You may want to try sterilizing everything (both the tools and your tissue culture media) in a pressure cooker or insapot before doing the transfers if you're having issues with contamination. I usually pressure cook everything at 15 PSI for 15-20 minutes. I have a few videos about this subject on my channel :)

@blakeamiller Год назад

Do you have any recommendations for how to work with plant hormones that come in powder form? These are the only kinds I can find that are readily available in the UK!

@plantsinjars Год назад

Sure! For BAP, Kinetin, and 2iP, you would weigh 25 mg of the powdered cytokinin, add 3 or 4 drops of distilled water, then add 1 M HCL solution a few drops at a time until the cytokinin is dissolved. Then add 250 mL of distilled water and transfer to a sealed container for future use. The process for making auxin stock solutions (NAA, IBA, and IAA) is the same, but you would substitute either 1 M NaOH or KOH instead of using 1 M HCL. Wear proper PPE and work in a well ventilated area with the powders :) They are banned in certain countries for a reason

@blakeamiller Год назад

@@plantsinjars thanks so much!

@filisildaanino5725 Год назад

Are you a Horticulturist???

@plantsinjars Год назад

@@filisildaanino5725 No I'm just a lady who really likes plants :)

@user255 Год назад

@@plantsinjars The PGRs are banned for large scale (agricultural) usage. Not for lab usage. Most of them are harmless unless very high prolonged exposure. You are more likely to mess up yourself with NaOH/KOH or HCl.

@kaylarogle 11 месяцев назад

Wow this is the coolest thing ever!

@glennnile7918 Год назад

Absolutely fascinating and indepth detail. Thank you.

@Skirmitch Год назад

Ok, I'll stop using my bare hands to set up my scalpels.....

@joegurt4782 Год назад

UNDER 200 dollars!?!?!?

@TechplantChannel Год назад

That's a good price especially when you look at how expensive it can be without a great guide like this!

@walterperrell4898 Год назад

Monsteras (decent ones) start @ $50

@nikkiknight6936 Год назад

Can be cheaper if you already grow mushrooms

@scinerd11 Год назад

@@lazy5373 considering a mushroom is more closely related to a newborn baby than it is to a plant, i struggle to see the relevance of this comparison. Obviously the needs of all three organisms are radically different

@constantinosbou Год назад

Very VERY good content! Thank you for everything! 🙏

@ROBBANKS666666 Год назад

It’s great to see a you beautiful woman who can be so smart and fun and not just take selfies.

@zephyrwinkle6552 Год назад

Fun fact that symbol on your plastic is not a recycle symbol. It's a plastic classification symbol. This was a ploy by big plastic they made a symbol similar to the recycling symbol to trick ignorant customers into thinking that all plastic is recyclable. While more than just numbers 1 and 2 are recyclable, 1 and 2 are almost always the only ones recycled because the cost of recycling the other numbers is way more expensive.

@raffaelerispoli1590 Год назад

Love it. Brilliant!

@urbanfarmacademy Год назад

Very happy I came to your page. I am a mycologist .. doing mushroom tissue culture for a while now and been really looking forward to plant tissue cultures and now I saw your page. Welldone and thank you for sharing

@h1234e1234 Год назад

Yes! I loved your video!

@skeletonboxers7336 Год назад

omg plants and tissue culture was THE hyperfixation i was doing before the tech layoffs started rolling through last year, sold off some clones and been job hunting since, but ur channel made me really happy to see this type of hobby again, honestly super excited to get back into it again after seeing this video. keep it up!

@mtz767 11 месяцев назад

Nice Work! That's exactly what I looked for!

@panpeter2969 Год назад

I am really impressed by the clarity of your video. And I look forward to see a follow up video how you separate those shoots and root them. Thank you!

@juliotrasferetti Год назад

Thank you so much for all this information!

@CrispenPayseur 8 месяцев назад

This is great inspiration. Thank you

@michealkish2902 11 месяцев назад

After watching other tc channels, and reading plants from test tubes. I like yours the best. In my opinion they do things that seem odd in process. 🤷🏻

@jonathanbigger4199 9 месяцев назад

You go girl and thank you I love growing and this is my next level

@dylancarter4928 Год назад

this was a hidden gem for me. dont regret clicking on this video

@socorrogonzalez5891 10 месяцев назад

Excellent tutorial!

@randyrodgers7848 7 месяцев назад

Awesome video was exactly what I was looking for. Thank you very much!

@lorlimann Год назад

Ok wow, very comprehensive! Love the tutorial ❤ I hope you recover quickly from your injury 😢

@ian11hendri03 3 месяца назад

Fantastic and very impressive Ms Laur. Thank you so much for the great video. lots of work and time needed to do editing like that etc. Hope your finger has recovered. God bless you.