I don't know why you would trust a random robot voice for pronunciation advice. Your original pronunciation was correct. Wikipedia is a better source. Mouse over the IPA text in case you don't know IPA. I guess using the wrong pronunciation is a way to boost engagement though... Lol ok just got to the "pippetTOR". I get it, definitely having some fun to boost engagement
Quick question! What would you personally use yourself?...8 Oz biocoupler or a 16 Oz biocoupler? Just looking to get one and didn't know which one to get...
I don't think the 16 oz would fit into a pressure cooker, but the 8 oz definitely does. I'll be using both in my livestream tomorrow night if you wanted to see a comparison side-by-side
"Why would your plants do all the work when you can instead micromanage their entire existence?" Haha, brilliant. I was looking to do this about 10 years ago for Aquatic Plants , but with the lack of info back then and supplies I decided to cultivate different exotic Mushroom species Now your making me get the itch back for the Aquatic Plant species as I have finally got some rare plants that I don't want them to be rare anymore to the hobby. Like yourself I started with the No Pour and Box with 100% Success. I think I might get back into it again as there's finally a club starting up soon and this would be a great opportunity to share genetics with plants.
Hello! Have you seen the research of lab-grown plant materials generated from Zinnia elegans cell cultures ? can you tell me if it's possible to replicate something like this?
For comercial growers TC is a big thing in the germination of many very small and high cost seeds, such as orchids, maybe you could try to germinate store-bought vainilla seeds or something like that, just for fun
What do you do with them once they are made? Are they able to be planted in potting mix or are they still needing to be grown out in sterile environment at this point?
The mushrooms they took the genes from glow about the same amount (or maybe even less) so even if you get it fully acclimated it probably won't glow more than you saw. They have the exposure turned up like crazy in the promo images and I'm pretty sure some of them are straight up photoshopped. But hey, it does glow, and that's pretty neat
Cool channel! Couldn’t you pressure cook in an old vodka bottle? I use to do this with my agar for mushroom cultivation. Then pour in a still air box or laminar flow hood. I would recommend making it in a glass alcohol jar then using isopropyl alcohol to spray down the containers. Could even use a few minutes of uvc exposure on them to kill even more.
I'm coming from the world of mycology and had no idea that tissue culture was a thing. You have earned a new sub. Excellent content. I'm looking forward to adding this to my new list of hobbies.
Typing in "Oh I hit my finger so hard".....Hmmmm, not the results I was expecting LOL Stumbled upon your channel, love the videos, not I'm gonna end up spending money for a home lab i didn't know I needed, but now really really want 😂
I tried mixing media before, but none of my explants callused or formed shoots. Though a few did eventually grow tiny new buds and leafs after 3 months.
Ask for a small scales and mention "its for use in my lab" the looks aee priceless... PH probes are great... make sure you keep the batteries fresh or they'll give incorrect readings, I use PH paper.
I would like to see cymbidium orchid seed pod sowing, as I will be having 10 seed pods ready in 9 months time in which I will be sowing myself. Try to approach a orchid society in your area and ask some growers if they know how to obtain seed pods.
TheGardenGirls on Etsy are the absolute worst! Just trust me on this. I have purchased multiple times from every Thai Con seller but the girls. One time, 20 plants, every one died. I make the others work using info I learned here from this wonderful girl. But those Etsy GardenGirls are thieves
I once did a guide on making no-pour agar on the shroomery forum, many years ago. It was almost exactly the same as what you do here :) What a blast from the past hah, we even have the same pressure cooker. That thing cost me an arm and a leg to ship to the EU.
As far as I know, you pronounced cytokinins correctly. (All of my professors in the past have always pronounced it the way you did the first time, not the way that the AI voice pronounced it). Thanks for the cool video. Oh, 1 question why sucrose, and not fructose? Thanks!
Sucrose is the most commonly used sugar in plant tissue culture, probably because it is the most common sugar in phloem sap. But it's not the only sugar that works, for example, glucose is used in many protocols for micropropagation of _Quercus_ spp., especially during root induction.
