Elispot assay ( assay to determine cytokine producing cells) 

Really nice video. It let us know both, things common between ELISA and elispot and also what makes them different from each other. Such animations in your videos are always helpful to remember things in a better way.

U r a blessing to the biology exam aspirants 🙏... Thanks a lot sir

Very insightful, helpful and nicely done! Thank you for your work, aand I hope you are feeling appreciated.

Very good animation. U forgot to mention abt stimuli. U need to add stimuli so that it will stimulate the cell to produce cytokines.

Very helpful & easy to understand

Brilliant video, extremely helpful

very well explained helped me a lot thank u

Serum and plasma are acellular. You need to use PBMCs for this assay.

So in ELISPOT, no of spots indicates no of cytokine secreting cells, however, I am not satisfied with this theory because I feel that one cell can secrete multiple cytokines, for example the IFN cytokine represents TH1 cell, and since TH1 cells secrete multiple IFN cytokine, wouldn't it be wrong to state that each no of spots indicates no of cytokine secreting cells. please help me clear this doubt

What is the advantage here in comparison to flow cytometry? In flow cytometry we do not look for "shadows" after cells, but we can actually count how many Th17 cells are there and it is much easier and faster than Elispot. Also in Elispots we do have SFUs often and it is hard to say how many cells were really there. I just don't understand why not just using flow cytometry? If someone uses PBMCs they won't know which cell really produced IFN (many do it) so Elispot will show in this case only shade of a cytokine and not the producing cells (we won't know which cell produced IFN in such case). An honest question, because I am new to Elispots and old to flow ;) I found my answer: "Flow cytometry can determine the number of cells that produce IFNγ, but not their secretory activity and the amount secreted by them. ELISPOT quantifies the frequency of IFNγ-secreting cells, but not the number of producing cells nor does it measure the amount of IFNγ secreted from cells."

Can you please clear a doubt? One cell can produce many molecules of cytokines which can subsequently bind to more than one capture antibody, and hence produce more than one spot. So why is it that one spot equals to one cell?

Very nice explanation!

Very Nice video, thank so much 🇩🇿

keep on going,we support yoy

Very nice explanation sir ☺☺

Sir if i want to analyse that which cytokines are release from the particular immune cell in respnse to a particular infectious agent which approach i can apply?

very informative

You are gem Arpan fantastic

Helpfull👍✨️

Thanks! Will done!

Plz send some research area for elispot cytokine secreting for therapeutics and vaccine

GOD bless. Too satisfied

Sir....could u plz clear my doubt ELISPOT is used for measuring cytokines or the secretory cells like T-cells??

Sir in which diagnostic center this test is avaliable sir

Ah, Biorender :)

Love ya

jiayous

Unfortunately I do not understand the English from Indian people

you are reading not explaining brother we are getting clarity on the topic 🤨