With a heartrate of 55 bpm, youre already in great shape. Ill see hiw NMN works on me. Im 59 yrs old. Cell age of a 66 yr old. 23 lbs overweight. BMR of 28.8. 44 inch stomach. My NMN will be here in 4 days. I'll share some more in-depth numbers next week. This will be a 90 day, age correcting test. 😊
Thank you for these videos. They are fantastic. Since your calories and diet didn't change, do you think the weight loss relates to NMN intake? It would be interesting to see if that was LBM or fat.
Thanks Sid21. In looking at correlations for BW with these tests, probably not: BW vs: DunedinPACE: r=0.59, p=0.30 Horvath: r=0.62, p=0.27 Hannum:r=-0.73, p=0.15 It's only 5 tests, so I'll need more data, but that's what it currently looks like. To run the correlations with body composition, I'd need DXA data at every test, but I don't have that, unfortunately.
I think the challenge at this point is that your diet is so optimized, that no single change you make is likely to produce a difference larger than the margin of error of the test, making false negatives highly likely unless you test over multiple time periods. I don't actually know of a solution for an n=1 experiment, unless there is some way to spend extra money on a super high accuracy test.
Thanks John. For the 49-day period that preceded Test #3 on 4/24, there was no NMN, but instead, serine + B6. The B6 part may raise NAD, so I also sent NAD for analysis on 4/24...
I don't, but the list is short: L-thyroxine (137.5 mcg/d) Vitamin D: 1000 IU/d except for spring, summer Serine 2g/d, B6 (PLP) 10-15 mg/d (bulksupplements.com, not affiliated or sponsored)
Great video! Thanks for sharing all this great data. QQ, Have you seen any test variation data from TruDiagnostic on their various tests? i.e. using a single blood sample, divide it and test it multiple times - how close are the results across the multiple tests? The challenge I see with using epigenetic tests is that without knowing the variability range for the test you can't know if your results are significant with respect to the test's variability. The only way around this that I can see is to submit multiple blood samples drawn on the same day for testing (cost prohibitive at this point) and take an average. Would love to know your thoughts on this.
Your nad only went up only when you started doing 1g NMN as opposed to 300mg, so you effectively increased nad only a week prior to testing which maybe just isnt enough time to see changes in the methylation patterns.
That's definitely possible, but also note that there was no impact for NMN on any other blood biomarkers: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-sSnK2YzlOfk.html
you are the site that i trust most to look into nmn, and this is the video I have been waiting for, and i am delighted YOU are doing these tests. I take a 1000 mg of nmn a day and I feel good for a 75 year old. But, is it the nmn or my CR or good sleep or healthy diet etc. My gut feeling is it works, or have I just been brain washed by the propaganda from the sites that are earning a commission on sales?? Please keep up your fantastic work. One of the websites told me of a few blood test marker than improve with nmn, but mine have moved in the opposite direction. I would love nmn to be good, not just expensive snake oil. John Hancock
Thanks for this. Where is the reference that shows that the Horvath/Hannum clocks are used for intrinsic/extrinsic age results from TruDiagnostics? It’s not mentioned for TruAge Complete.
I'm not sure why they didn't mention Horvath or Hannum on their site, but IEAA and EEAA are defined as Horvath and Hannum's tests in this paper: www.ncbi.nlm.nih.gov/pmc/articles/PMC5076441/
would prefer you tried CAKG for 7 months, like the Rejuvenant study. I am currently doing the MyDnage test but just started supplementing, already sent in my blood sample for the baseline Horvath
Hey Jay, I couldn't be more bearish on AKG. Its lifespan-extending effects were not impressive (no mice lived longer than 36 months), and the epigenetic study was small, and could be epigenetic noise, especially when considering the 3 - 7y variability in my data for Horvath's test.
42 individuals is a lot for epigenetic noise . 8 years is a mind boggling amount which couldn't be explained by noise. You may be right in the end, as this is one study, but there is no other anti aging supplement on earth that has had a similar effect. I will report my results in about 6 to 7 months.
Have you considered going completely 'off protocol' for 3 months and eating Ad-libtium with no supplementation and then re-testing to get a new baseline, so you can see if these factors actually impact your results on the clocks?
Ha, no way. While that would prove some points, I'm more interested in getting to my best biomarker profile and staying there. While that won't prove causation in my case, I'm ok with that.
Hi, I would like to see you take a small bloodsample to test if NMN has an effect on PBMCs, as health indicator for immune cells mitochondrial respiration and glycolysis.... I would really appreciate it.
My personal experience with epigenetic age testing tells me it's not very reliable. At the beginning of 2021, before I started testing the effectiveness of NMN on epigenetic age, I took a test on Jan 10th 2021 with Epiagingusa. the result came back with epigenetic age of 40.7(I was 49.3 y at the time). I started taking 1g of NMN on everyday, half a year later, I took the same test July 10th 2021, but the result is quite different with a epigenetic age of 44.2 - a 3.5 years difference in the wrong direction. I didn't change other aspects of my life beside taking NMN, so I sent an email to Epiagingusa questioning about the big difference. They responded by giving me another test, about 20 days later, and it came back with an epigenetic age of 45.7, even older. I was quite disappointed by the results, and don't know what conclusion I could draw from them, but because of the experiment, I hence have stopped taking NMN, but I cannot stop wondering if I should have trusted the results for my decision to stop taking NMN
Hey Andy, there are lots of factors that can impact epigenetic age, which is I showed data for diet and activity levels at the end of the video. In terms of reliability, the epigenetic age range is relatively tight for me over 5 tests, as mentioned in the video for each of the 3 tests.
@@conqueragingordietrying123 Also, as someone mentioned here before, cyclic viral loads (wth this new possibly lab-developed gain of function RNA to apply stress on humanity (what they think are needed for crisis management in financial control and governing)) influence the test results a lot. Our endogenous antioxidant systems go out of balance possibly, an immune memory keeps it going for a while.
Oral NMN is not very bioavailable according to the literature. That is why an oral dose of less that 1g is not effective, supposedly. Most interesting would be the data markers indicating your cellular NMN levels prior to supplementing, then after supplementing for a couple month, what is your new NMN levels. Your gut biology may not allow any NMN...or the opposite. Recently Hyogo Medical University in Japan showed that NMN by IV massively increased NAD+ and reduced blood triglycerides. I am not advocating IV route for NMN, but for methods to increase oral bioavailability...such as taking NMN with combinations of lucine metformin or arginine or other methods that facilitate better absorption.
@@conqueragingordietrying123 That change from 25 to 39uM is nice. I'm very happy seeing htat. Maybe some of the markers, also epigenetic, is somewhat tied to some immune imprinted patterns, so it takes some time the change the memory (tons of antibodies, auto-antibodies and antibodies to auto-antibodies etc in play there).
I don't think NMN has ever been shown to increase lifespan - rather healthspan - so perhaps this makes sense. Though IMO a week at the higher dose may not be long enough to detect anything meaningful.
@@N330AA Yep, but even if NAD was only higher for 1 week, I'd expect some biomarkers to change. Nothing did, including epigenetics. I may have to go way higher (25 - 80 uM, instead of 39 uM) to see an impact. Let's see how B6 impacts NAD-that test will be tomorrow.
@@conqueragingordietrying123 Maybe the microbiota in the gut that metabolizes NMN have not come along yet :). Though I think so many species probably metabolize it that it shouldnt be a bottle neck, hmmm
Is there a chance you are not NAD deficient so therefore are seeing no impact with supplementation? You may be a bad test subject to establish a proper correlative study as you are already on the extreme end of the curve similar to the way lab mice with long telomeres skew drug safety testing.
That's definitely possible for most of my data, but when considering NADPH's role in DHEAS production, I think that NAD has a role. The question is, how high will I need NAD to bring DHEAS back to youthful levels? We'll see, i send blood for analysis yesterday...
Nope, 2g/d, which was a 50% increase over my average intake. ~7g/d was given to older adults with suboptimal biomarkers, which currently isn't the case for me. Also, the goal was for glycine to be converted into serine, to then help with conversion of homocysteine towards the cystathionine-glutathione pathway.
@Conquer Aging Or Die Trying! plan on taking gylcine in the future? I have Nafld. I'm hoping a nac/ glycine combo 1.5 grams each , excersize, and diet will help
@@Nando_lifts2021 Constant NAC consumption possibly not ideal (?) - as in non-human tests it tilts the redox balance (not enough ROS left to signal the endogenous antioxidant pathways)
Would two weeks on NMN be sufficient to show any major effects? I took 900mg (3 tabs) of Tru Niagren NR daily for a while, but recently got a couple of hundred grams of MNM and have switched to that. I do not manage to maintain as rigorous a dietary or supplement or testing regime as you manage, and thank you for carrying out this research. I have relied mainly on calorie restriction for the past 30+ years, which I began in 1990 and which is (I believe) why the genetic-based hyperhomocysteinemia I inherited from my father didn't also kill me in my early 50s. As hyperhomocysteinemia basically causes accelerated aging my main therapies against it are senolytics and 'longevity supplements'. I have two intertwined battles - homocysteine and aging. What has really surprised me in my research is the possible massive numbers of people potentially with undiagnosed hyperhomocysteinemia, due either to minor genetic polymorphisms or poor diet or buggered gut microbiome, especially if you go by the most current research and see 10 umol/L rather than 15 umol/L as the normal/safe upper limit. It is just so under the radar of practicing physicians though, possibly as early trials lowering homocysteine in heart patients didn't lead to a drop in heart attacks or strokes and because for treating elevated homocysteine you use mainly B vitamins and not pharmaceuticals such as metformin for raised blood glucose or statins for raised blood cholesterol. Also lowering blood homocysteine is a preventative medicine action, with conventional western medicine more interested in treating the conditions such dysfunction causes rather than stopping them from developing. Unless there's a buck in it I suppose, as evident by the mass prescription of statins to those who had high cholesterol but had no sign of heart disease.
Maybe NMN (and CR) does not affect those metrics of those tests that attempt to measure epigenetic age. Look for changes outside of those tests. e.g., My white hairs are growing out black again, etc.
Outside of these objective metrics, I haven't noticed any differences for hair, skin, nails, sleep quality or duration, or workout performance with the 6-week NMN supplementation period.
@@N330AA Ha, I haven't spent much attention on hair loss or skin aging, those are weaknesses in my approach. But, those aspects won't kill me-I'm going after that stuff 1st
@@conqueragingordietrying123 I've been taking 1000-1500mg of NMN per day for 5.5 months and about 1-2 inches of my white hairs have grown black from the root. Given the low risk factors, if that's the only change for me, that's enough. Those epigenetic age tests are based on data on the "normal" population. Those tests do not take into account the few people doing age-reversal protocols. (There's a guy optimizing his epigenetic test results and he looks like a freak. It's like trying to ace the SAT's vs being usefully intelligent in society)
There is a private funded NMN human study taking place in Europe right now (Afega) but it seems that it will not give satisfying results, probably because of the impact of Covid19 on us. People who had an even mild Covid infection experience an accelerated ageing process, measurable with the epigenetic tests, and they also have significantly higher levels of senescent cells then before (there is a scientific study confirming this). I am one of many, had two times mild Covid and developed Long Covid and probably also an autoimmune disease (analysis are still being processed) after the second infection last year. The three vaccines l had got before have not really helped me, they also might have counted as three mild infections for my immune system. Now l have to get rid of this🤬
I agree with all of this. That was actually the first thing that came in mind, that anyone doing the aging tests at this point in time need to count for the fact the both vaccines and possible viruses age us a lot quicker. Vaccines actually did raise the viral load on everyone of us, even for those not vaccinated - it allowed more proliferation of viral particles and more of these in environment, more sickness around us. BTW, can you please give us the title of the research paper discussing the covid accelerating the aging measured with epigenetic tests (I think you cannot put links here, not sure, so the title will do, if at hand).
For me, maybe not. There's a possibility I didn't go high enough, but that experiment is on the back seat while I try other methods (B6, for example-testing again tomorrow).
@@conqueragingordietrying123 oh it is lot oi money investing on NmN and no results .. but the Man In The RU-vid vlog THe PULSE defends NmN use and DID YOU TEST THE QUALITY OF THE NMN BECAUSE THEY CHEAT TOO
I'm purposefully trying to get leaner, not heavier! Around 140 lbs, I'll be close to 6%BF-that's the goal, for now (unless biomarkers start to look bad in the process).
