thank you so much sir....your videos are very useful for research work......if possible please make a video on malondialdehyde and lipase of flour samples
Do you have any video in which you explain the calculation for the analyzed compound's concentration based on the sample amount I used? This is because I should consider the amount of plant same I used for analysis as well as dilution factor etc..
I have seen your DPPH, phenolic,methanolic extraction of plant sample and FRAP.All are very good.How beautifully you explained everything.It is commendable.I am also doing my work on plant sample. If possible could you make a video on SDS-PAGE. Especially on sample preparation using plant sample.Thank you
Sir.. total volume from: stock+D/w = 1000 micro litres (or 1ml) Or stock+D/w = 500 micro litres (or 0.5 ml) ... You told to make , total volume should be 500 micro litres (or 0.5 ml). Is should be 1000 micro litres (or 1ml)?? Please reply
Sir, I'm doing research on pigment extraction using different organic solvents from flowers . Can I use the pigment extracted directly for phenol estimation without drying to powder form?
Thanku sir for this video...but I m confused how should we decide the exact concentration of our sample in methanol or ethanol which needs to be estimated. Kindly help
After evaporating say for eg. U get 0.01g of extract that is 10mg. Add 10 ml of solvent. Now ur conc is 1mg/ml. Like this based on what u get u can add to get 1mg/ml, 10mg/ml 5mg/ml. Everything is crct
Sir will you plz tell me that if i am preparing Gallic acid reference standard solution for standard curve preparation for phenolic content determination then if i have taken 10mg Gallic acid in 10 ml distilled water and then taken 6 concentrations from that 10mg dissolved in 10 ml distilled water solution for taking absorbance for making standard curve then is it correct way of making standard curve . And sir there is no need of adding working solution in standard solution for making standard curve?? Pls help this is for my research work
There are 2 methods, you can do the method i said its easy. The other one with working solutions and all is bit complicated. Yes 5 concentrations are enough. You r doing it correct. All the best 👍
Sir, you added 1 gm of gallic acid to 100 ml of distilled water. Then how can the concentration of 1mg/ml make? please explain this and rectify me if I am wrong here.
I am using choline chloride:urea deep eutectic solvent for extracting phenolic compounds. The problem is that when I add folin reagent to (gallic acid+deep eutectic solvent) standards prepared, it form precipitate or colloids which settle down at bottom after some period of time. It doesn't happen when I use water as solvent. Do You know why it i happening?... Also I use 7.5% NA2CO3 for estimation.
Can you please tell me how to make a plant extract? I mean can we use oven-dried plant material at 60 degrees C to make an extract? If so, what solvent can we use?....any help would be appreciated.
Stock soln of Gallic Acid and water is prepared, now I hv to prepare standrd solution for stndrd curve, so may i know wht compunds we use to make standard curve other than stock solituon?? Please reply...means alot
Sir, how to make dilution of folin ciocalteu reagent (from sigma Aldrich) , it's written 2N on the bottle ... What is the desirable conc. For standard preparation for phenolic estimation.?? Thank you
1:1 or 1:2 both are ok. I preference 1:1. For standard you need to do trial and error. We can't follow strictly or I can't just say some concentrations.
@@Dr.Rakesh.B sir, I have tried making standard for phenolic estimation, i used all the reagents and their conc. in the same ratio as you have mentioned here, but every time getting high absorbance value while making standard.... How to resolve this... 🙏
@@Dr.Rakesh.B Thank you, sir. Sir if you don't mind can you please share the references for phytochemical analysis (quantitative estimation) & for methanolic extract preparation?
Actually you are supposed to use methanol only. Thats logically correct. In some cases we do slight modifications like this if we are having shortage of chemicals
When we are taking the absorbance in Spectrophotometer then if we are taking samples and all the chemicals by multiplying 10 to the volume you suggested in this video then is it correct or not?
1:1 dilution i did. I have read papers which showed 1:10 dilution also but it depends on the concentration of the chemical , purity and brand. I suggest you to do 1:1
You can get my papers in research gate or Google Scholar. I have made a separate video on how to get my papers for references. Please watch thT from my channel.
Sir, I have a question, I have standard graph of pigment prepared in Petroleum ether. But for other assay, I have to solubilize in another solvent like methanol, In that case if I dry Petroleum ether extract of volume 1ml with conc 50ug/mL and solubilize in 1ml methanol will the concentration be same but OD will vary? Please send me some suggestion
It's mentioned in video if I'm not wrong. Kindly watch without skipping. Or watch videos of other biochemical assays to understand blank and control concepts
@@Dr.Rakesh.B thanks 4 reply but sir concentration per ml or per 100ml would be changed. during calculation after calibration curve which concentration will be taken into consideration with respect to only gallic acid or total solution concentration
Sir when I apply in ethanolic extract there are some turbidity problem in my different samples and spectrophotometer increase the O.D. how to deal with that
Sir, As I did same but I got precipitate in the blank as well as In all samples after adding Sodium carbonate Do u know the reason if so kindly tell how to prevent from this My 2nd question is If we take 1 ml FCR reagent then we have to simply add 1 ml of distilled water or we should dilute it 10 times
I took 4, 6,8,10,12,14,16,18 ul from stock solution (0.5mg/ml ) n draw standard curve 1 got 10.40 of my Sample after applying y= mx+b I took 10 ul from Sample (which is having 10 mg/l) So my question is what will be the unit of my Sample of which I got value 10.40 Will it be 10.40 ug/ml ? Or does it mean that in 10 ul of my Sample there is 10.40 ug ?
