Gene cloning is the process in which a gene of interest is located and copied (cloned) out of all the DNA extracted from an organism.
In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) Selection of organisms containing vector sequences & (7) Screening for clones with desired DNA inserts and biological properties.
6 сен 2024