I prefer to cultivate resilient mycelium...need to keep them exposed to the real world a bit to build up their immunity! lol but yeah, sometimes I feel really lucky for how few cleanliness precautions I take and how good the results are.
Lol if you master whatever sterile technique that works for you, you really dont need gloves his channel is proof. As long as your clean your good and your work speeks for itself in my noob experience. Obviously gloves are better then no gloves but if your smart you can be successful without them
He hover his hand over the lid of the mother jar. He cleaned his hands and tools well but.... that's bad technique if you ask me. Movements should 100% be from the side of stuffs never hover over openings as gravity will take any contamination particle he got left on his hand down to the clean spawn.
Have you ever recorded the percentage of contaminated specimens from the SAB? I've seen others saying that the rate of contamination with a laminar flow hood was between 5-15%
Interesting... most people, especially sellers, keep saying that a laminar flow hood stops any and all contaminants, by way of how filtered air flows out from, though some say there almost as many as sabs.
@@stephendekoning802 That also is interesting. I know each unique environment has so many different variables. Since posting this comment I've learned that many "laminar flow hoods" are not actually providing true laminar flow. They should be calibrated or dialed in with a variable speed switch each time they're used. I would venture to say that the people that experience the higher rates of contam with a flow hood, probably had turbulent flow and/or maybe had too much confidence leaving their cultures exposed too much
@@ilovehihatsIt may be, but if you are growing mushrooms to market them you should never stop cleaning and sanitizing everything daily and do a deep cleaning once a week. Since, beyond the fact that losing a mushroom harvest due to contamination is an unfavorable situation having a potentially deadly bacteria in your mushrooms, such as listeria which has a high mortality rate, it's way worse, many people could die.
Yo….. I have a 3LB spawn bag probably a few days away from being fully colonized and just got some new jars… I’m gunna try this. Is there any risk of contamination for the donor spawn ?
Huge risk for donor spawn, don’t understand why people do grain to grain at all. If you’re gonna put anything directly to grain that isn’t liquid culture it should be clean agar under a flow hood. If you have spores, agar then first then go agar to grain or agar to LC the LC to grain. Grain to grain is one of the worst methods out there for the donor and receiving grain.
@@danielodonnell6334 get a liquid culture syringe. There’s a lot of equipment and steps needed to go spore to agar to liquid culture under a flow hood but if you can get a liquid culture vender that’s all you need
