Inoculating Agar and Grain in a SAB (Still Air Box) 

I'm still gonna use alcohol

You still have hands?

Lesson learned the hard way myself. 2nd degree burns on my neck and my hair is scorched to the scalp in some places. Bleach works fine, and alcohol on towel inside if any. Wipe items before putting in the box.

lolll glad I'm not the only one, I just flashed my box like 2 or 3 weeks ago, my right hand is messed up but it healed now. Well, at least the box is extra sterilized from that flash. I actually thinking of a safer way to flash my box like that again because in my theory the flash will push any particles out and the heat will destroy any microbes inside.

I did same lol-on a very small scale lol

It’ll definite cause some burnt eyebrows lol

90 second mycology mentioned it i believe.

That’s one really great safety tip I’ve heard no other videos mention . Thanks man for this video 🤘🏾

using a flame in the still air box also creates air flow because of the heat of the flame (convection)… so partly defeats the purpose of the SAB

We probably need some boom videos to go viral before this info will be widespread common sense. I've only heard horror stories on the shroomery forums.

Thank you I was confused

Got confused syringe is now in my ass, what do I do now?

Freaking great tip... i was just thinking of what would work best.. thx for sharing that...

This is an awesome little hack! Thanks for sharing it

that is an absolutely brilliant tip for any newcomers that may not have a drill or hole saw. Wish I could like it twice.

Hey does it Go through pretty easy or have to reheat a Couple times to make the hole? Also what size Tub do you recommend?

I just got my sab and wiped it all down alcohol 70 same for the out side of the all in one grow bag put it an my spore syringe in I had a new in the sealed package needle so I didn't burn it hope that was not a mistake and it set it up all in my closet so it can stay in the dark and at a 70 degrees I picked a strain called butters but now I don't have a clue what to do next

That's actually a great point!

I'm going to try using a damp towel soaked in water with ~5% bleach to seal the floor of my SAB and just leave the lid off completely. I saw MycTyson using this method.

I lay aluminium foil down and wipe it down I don’t trust the lid

I usually use one of those grated cooking trays. Super cheap and available everywhere

@@bigbrotheriswatching2680 how did it work

Just make sure to not mix the two, bleach + alcohol = chloroform :)

@@urmommaahugehoe Whatever man I can handle my inhalants

@@HavelockVetinarii -how 0-0

@@emilys2849 ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-uK233oUMUsk.html

@@HavelockVetinarii lol

Yes, syringe is only marked to 10cc's

Only if you're pouring your own agar and sterilising dishes

Frig off Ricky!!

That's absolutely possible! Mycelium expansion increases exponentially. You could then turn that into thousands of pounds of fresh mushrooms given enough space and resources.

@@midnightmushroomco.2091 Thanks for the reply!

Possible, but pros no that no more than once again. Fine results. Move some to new bags...not as much production and slower. Do not move it a third time. Genetic drift and more, it is degrading with each generation. Not noticeable when going one jump.

My pleasure!

Thanks!!

Shouldn't be hard at all to make clean agar. Maybe it's where you're storing it that is making it dirty. Look at no-pour teks if you still have trouble, but put your agar in new (already sterile) ziplocks or even just spray the insides of already opened ones with alcohol or Lysol before putting in the plates.

It's been a month so surely you've used yours already. But I should add..making clean agar is easy, especially with no-pours. Ordering clean agar won't help if technique is bad, and storage is bad.

@@c.odubhlaoich2948 it's easier to keep grain from getting contaminated then agar. I think when making agar you almost have to have a flow hood, which I don't have yet. For now I have a s.a.b. Hopefully soon I'll be able to have the space for it.

@@shawnwillis7561 Are you talking about for pouring or doing transfers? You only need an SAB for transfers, but like I mentioned, you can do no-pour agar which of course requires no SAB to make because it's already sterile inside the containers.

It looks like it's around 88 quarts. You want somewhere between 80quarts to 120quarts max

he said in the video to use a 50% bleach/water solution (1:1 ratio); I highly recommend NOT doing that as it's noxious. 1:10 bleach to water ratio is sufficient. You can google around for bleach concentrations needed for sanitizing. They'll often use even less than 1:10.

They tend to be tapered and wider at the lid than the base. So flipping them gives you the most table area inside them

@@HeyHeyHarmonicaLuke Humm, thats true! Thanks

Spore syringe is easily acquired. Liquid culture is normally made from an existing fruiting body through a process that can be found on RU-vid. Liquid culture is a nutrient rich suspension medium while a spore syringe is just sterile liquid that spores float in until use...from what I have observed. I started with spore syringes simply because it was the "novice starting point". With more research I moved to liquid culture and agar transfer.

@@Redweezle Thanks

@@Redweezle Is the reason people use a substrate for price reasons? Or is there another reason people don't just use a huge bag of grains for example and fruit using the spawn medium?

ideally the cut sides of the parafilm never come close to the opening of the containers... They stay closed, you can contaminate the outside surface all you want, the parafilm keeps it closed anyway. Never had a problem of contamination going across or through parafilm if that's what you're worried about

Alcohol sanitises it's doesn't sterilise.

@@k-lab3824 Thank you. Sanitize not sterilize.

you can build one yourself for around $180 son. Start saving your allowance, pick up a paper route this summer, and when you're ready some nice strangers on the internet will offer you some assistance

Mixology

I’ve never had that be an issue. The moisture is drawn into the grain in about a day but I use them as soon as they cool down.

Great Informational video! Thanks

It’s from a lab equipment company called GermFree. I bought it on auction from a university

Thanks definitely go to

Actually, a lot of people unknowingly set themselves up for failure installing thick gloves into the box. Gloves create a billows effect that pulls in air from around the lid of the SAB. So when you move your arms around with the gloves installed you’ll literally pulling in dirty air from outside the box. Having no gloves avoids this all together.

@@midnightmushroomco.2091 What if the lid is air tight? Does this still apply?

Thanks!! I'm working on some more shorter videos about techniques and procedures I use. Simple stuff that keeps my processes going without too many issues.

Yeet

It’s highly dependent upon species. Some spores germinate quickly and others take a while.

So you just spray water inside?

@@burymebelowawillowtree9243 I spray isopropyl inside... Keeps it moist and clean... If I'm gonna work with flame then Clorox because I don't want a boom lol

@@miguelsuarez-solis5027 thanks!

@@miguelsuarez-solis5027 What mix of Clorox and water do you use?

@@carlfranz 2 tsp of Clorox to 2 cups of water

#dontstabyoself

Yes, the SAB minimizes the amout of airflow though.

That’s exactly what you want it to do. Molds have spores. I’m not getting the bleach in the agar or bag. It’s simply to kill any mold or bacteria in the outside of the plates and bags.

I see. Still learning here. I would be worried about it dripping from my gloves or rolling off the lid or something.

That’s a very valid question. In the open air there’s still air currents that carry spores and things around. Inside the still air box we try to let things settle and hopefully keep contaminants at a minimum.

You would then cut up the agar with a sterile knife or scalpel and put into grain.

Hey How much of the agar would you use for a 3lb bag of Grain? I’m in the middle of Putting an Order together on you site..Thanks

Correct! They are typically gamma irradiated by the manufacturer. That’s why nurses don’t flame sterilize before giving you a shot. 🙂

You know him he doesn't like extra steps.😄

He used normal tape there, I believe his bag had an air exchange port up at the top but I’m not sure if it’s needed or not.( my guess is yes)

It's literally just regular packing tape. I've used injection ports but this honestly works just as well.

I can't see why it would be an issue, with bleach, more the better

Frig off Ricky!!!

😂

A flow hood is much better and you can likely use alcohol in it. The reason alcohol is bad in a SAB is the alcohol evaporates and sits in the box. Add a flame and BOOM! Well a gush at least. In a flow hood the vapor is pushed out.

Working inside a SAB you want the surfaces wet to hold down any potential airborne contaminates, in front of a LFH you want dry surfaces so airborne contaminant blow right by.

Smokes let’s go..

We 100% guarantee the quality of all our products. If they came in the mail contaminated then we’ll absolutely replace them. Please reach out with your order number. Thanks!

Lysol is more flammable.

I get my from an online store, they got shroom, psychedelic and microdosing products well refined, also ships to anywhere.

Mycopete.

You heard right and he is a bit off on a couple things. f you look at the label on any cleaner it will give you the optimal ratios. If someone does not believe the label, ask yourself this...why would they lower a ratio when increasing the raito would have you using more product and earning them more revenue. It makes no sense. Generally they will give you the best mix for the job at hand, One change to his video would be to let the bleach mixture dry. For the most part, none of these compounds kill on contact, they need time to penetrate the organism to kill it. Just like alcohol, you need to let the material sit for a bit before you wipe it down...if you do at all. In SAB work and when it comes to contam, slowing down a bit will go a long way.

rare roachdoggjr spotted

You could but I never do.

A SAB with attached gloves are actually worse. The gloves create a billows effect and will suck in air around the lid of the box. This completely defeats the purpose of a still air box.

@@midnightmushroomco.2091 thanks

Glad you enjoyed it!

It’s sterilized. Check my channel for a video on how to easily make grain spawn.