Gram Staining

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This video demonstrates the Gram staining method for identifying unknown bacteria. The method for mounting bacteria on a microscope slide using aseptic technique is shown.
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15 окт 2012

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Комментарии : 292

@poojapareek1386 4 года назад

We can learn this with a sentence Come In And Stain C- Crystal violet I - iodine A- alcohol ( ethanol) S- safranin

@hollywoodisasataniccult4022 4 года назад

Pooja Pareek yo thank you!! Have my micro practical tomorrow. Im a Pharm d student in Pakistan 😊😊

@adnanahmed7463 4 года назад

Thank you for helping me remember this:)

@nocmemer4686 4 года назад

Pooja di thx,I have exams tomorrow it will help me

@poojapareek1386 4 года назад

@@nocmemer4686 all the best for your exam dear😊...and no need of thanks dear I'll be great full if I can help you 😊😊

@nocmemer4686 4 года назад

So sweet of you😇

@roselewis7683 4 года назад

this is one of the best representations of a gram staining procedure I have every seen. Simple and straight forward with all the precautions built it. Well done.

@raykyogrou0358 6 лет назад

Interesting! Never had to do this before, kinda wish I did. Only disappointment was at the end because I was kind of expecting to see what that slide looked like under the microscope.

@antonyshigin4977 2 года назад

Yeah...Me too disappointed......

@ProfRoofs Год назад

Here you go ---> ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-egpZ0BdJQE8.html

@nadaahmed6236 Год назад

@@ProfRoofs THANK YOU SO MUCHHH

@blackberrykathryn100 8 лет назад

Staining is to improve the contrast within a cell to show the organelles in greater clarity and detail. The cytosol is transparent there is little if any contrast. Using positively charged dye it attracts to the cytosol within the cell and you can distinguish between the different organelles. Using a negatively charged dye it repels the outside of the cell and stays outside the cell and distinguishes the cell from the background. The positive dye could be Methylene blue and the negative dye could be Congo red. Differential staining is used to differentiate between two different organelles or organisms. Gram stain technique is used to show gam positive bacteria and gram negative bacteria whilst the acid fast technique is used to show mycobacterium form other bacteria present.

@haleyknedeisen8108 3 года назад

I have a gram stain lab tomorrow and this was really helpful!!

@loeyexo1685 7 лет назад

thank you for this. i hope i'll do well on my practicals tomorrow 😨

@gargimahajan2273 7 лет назад

ᄏᄏ아라 So, how did it go?

@firephoenix8192 6 лет назад

I have praticals tomorrow as well

@iqrailyas2097 6 лет назад

i have practical tomorrow too

@hollistercal888 5 лет назад

same lol

@rvsingh4538 5 лет назад

Mine too

@nafisaeiramnoor3428 Год назад

I have microbiology exam next week. This video is so helpful for students of medical schools like me. Thank you for making it👏

@sugarcoder 4 года назад

Thank you for making this video! My lab partner hogs all of the lab activities and doesn't care about other people, so I could not learn it well sadly. There is a lab exam on Gram staining, so I am glad that you made this video so I can learn how to do it!

@jellykin7161 7 лет назад

Nice video! I wish I had seen this *before* doing Gram Stain for the first time as a high school student. Instead, I had to rely on an ambiguously worded worksheet for instructions. I ended up washing my slide completely clean of all cells... That was spectacular, I suppose. ah ha ha :)

@stookiegrace1253 6 лет назад

Joules Kin same bit somehow my e. Coli cane out gram-positive which was correct!

@sajanvincent1111 3 года назад

Thank u for the information

@haninasofeafaizal3600 4 года назад

nooo i wanna see the results :'(

@srija_7890 3 месяца назад

😭

@KevinRaizada 2 месяца назад

Me too

@arian138 2 месяца назад

Thank you for saving my time, not to see the video. You lost but you saved many.

@irregretableyblessed2581 5 лет назад

quick and straight to the point explanation

@_Dr.P_ 3 года назад

I have my practical exams tomorrow And today I am watching 😂😂 So thankful to you for explaining each and every step perfectly Hope I will do well tomorrow 😂😘❤️😂

@A9poj 2 года назад

armyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyy

@_Dr.P_ 2 года назад

@@A9poj ARMY 💜💜💜💜

@himanshurao8668 11 месяцев назад

@@A9pojstupid armyyy

@fatmaahmed832 9 месяцев назад

Thank you for this! you saved me from the embarrassment of asking my lab partner who likes to gatekeep information to himself

@jansherkhan5835 2 года назад

Awesome. Passionate to perform this on lab.

@SurvivalSquirrel 11 лет назад

Where is the observation?

@blackberrykathryn100 8 лет назад

So gram staining is to differentiate between gram positive and gram negative bacteria and acid fast technique is to differentiate mycobacterium from other bacteria

@JessM20_ 7 лет назад

Kathryn Russell yup!

@firozahmad1653 4 года назад

Acid fast different iate Mycobacterium tuberculosis. And m. leprae

@twilighteclipse7337 3 года назад

Oh! This is the greater Graim Strainig procedure I've ever seen. It's so interesting.

@scholasticasonia3806 2 года назад

Thanks I've understood the technique properly thanks to this video.

@jelenajeca4585 3 года назад

This is amazing, so interesting, I like it!

@KeyannaMoss 6 лет назад

Can you post all your lab experiments...lol I learned so much through this THANKS!!1

@Kidsent567 5 лет назад

If it is time i will post it dr

@BlackFlagHeathen 4 года назад

Ugh I hate lab practicals. These RU-vid vids are a godsend!

@MelomaniacEarth Год назад

I did it yesterday in my internship. I'm so happy.

@Jeene269 2 года назад

My histology exam after one day! This vdu was so informative thanks ✨

@sara-bz3cy 6 лет назад

thanks that was SO helpful 💖👏👩‍🔬

@edidiongekpo 5 лет назад

Thank you so much, I learnt a lot from this video!

@Cccvgd13 2 года назад

Thank you so much ,I learnt alot from this video!

@joybulan1800 4 года назад

Thank you helped me for my bacteriology subj

@CutiePie-qo7zu 3 года назад

Very clear explanation keep it up sir

@jhoannadipay5594 2 года назад

Thank you for making this video!

@gayatrisawant8127 9 лет назад

nic and straight technique

@niqabyousafzai6525 7 месяцев назад

thankyou, its all cleared. having exam of Gram staining tomorrow 🙌🏼

@souravsupakar6858 3 года назад

That was so interesting I am not a science student but it was like I can understand everything by watching this video

@pekhom6660 2 года назад

Interesting and helpful episodes.

@ashutoshprasad8494 4 года назад

simple and easy, thank you.😊😊😊

@Samuitsuki 3 года назад

Rinsing in a beaker seems a lot more controlled than using spray bottles as they seem to do in the lab I'm taking classes in.

@zemenuachamyeleh45 4 года назад

tank you for this good presentations.i got it good experiance.

@zebullon99 10 лет назад

Very clear and informative tutorial.

@sulimankhan621 5 лет назад

good

@MED.Laboratory 3 года назад

Its wonderful understand the Subject after this video saved me Thanks very much

@joodalzahraa6993 3 года назад

Thanks 😊 that was so helpful ☺

@nourassani5589 3 года назад

Wow so amazing your explain 😍

@nursepotassium 2 года назад

I have a gram stain skill exam in a few days…can’t wait lol

@anutamang9930 5 лет назад

Thanks that was helpful ☺

@rishabkumarmodi5959 5 лет назад

Post more helpful videos on equipments and related solutions which are used and how can they be used

@JoeMikeTennis 9 месяцев назад

Thank you so much for this video. ❤

@alipohsem1781 2 года назад

Amazing video

@reflexoplays6451 6 месяцев назад

we wash the slide with a bit of detergent before use to make it grease free which helps alot in spreading the water droplet and giving a smear of bigger surface area.........idk if it's right but it's a mandatory step in our university and it has become a part of staining procedure

@Rishidev2922 4 года назад

very useful video.tommorw my practical xam.

@mildredhart9510 2 года назад

Thanks a lot, would really be helpful in my defense

@yosnachatterjee7210 6 лет назад

Helpful...

@zeenatbashir68701 2 года назад

Good job 👍

@user-qc5nf2lh6x 3 года назад

Thank you for this useful video

@BioRadLaboratoriesCorp 3 года назад

You're welcome! If there are basic biology and life-science laboratory techniques you'd like to learn more about, email the Bio-Rad Explorer education program at explorer@bio-rad.com

@user-tl1kb2nd7e 2 года назад

Good job

@NobleTenz 5 лет назад

wish me luk on my practical :)

@bryanvelasco5567 2 года назад

this is interesting to watch goodjob 🎉

@pamelabitar9558 5 лет назад

If the stain of crystal violet came inti my hand how do i take it off

@martasoria6122 2 года назад

Thank you . Is very interesting

@DrAtifaAmbreen 8 месяцев назад

Great video 🫶🏻

@littlesneets8026 Год назад

i understand this video is 10 years old, but i just want to be sure. is the gram decolouriser Ethanol solution? because i'm sure that's what we used for our gram staining methods to rinse the stain, but maybe i am just mixing up the wrong reagents

@rashisahu15 3 года назад

thank you for this..i hope i'll write the same in my exam👍👍

@chandansarma9033 3 года назад

Thanks that was helpful

@yadneekadam5833 6 лет назад

The video was nice, but I have a question, u marked a circle with wax pencil , and made a smear on the same side???? As I'm a new student, my proff taught me to mark the slide , invert it n prepare a smear on inverted slide.... Why y did so?????😶

@Notsowell123 Год назад

haha I was waiting for what it looked like under the microscope

@isharamadushanka8736 8 лет назад

Useful one

@YoyoBear12 8 лет назад

loved it except for the smear. it was a little too cloudy in my opinion.

@erikhernandez6450 7 лет назад

i think it may have been agar residue

@pratibhagavit7857 6 лет назад

Tnks for this usedful video.....

@harshadasonawane7445 6 лет назад

Nice video

@thilinaalagiyawanna3680 2 года назад

Thank you very much

@VanillaSnake21 5 лет назад

How come when I apply the alcohol most of my specimens come out red, even something like gram positive bacillus subtilis comes out half red half blue and sometimes completely red. Professor said that could happen if I'm applying too much alcohol but I do it as instructed, until it runs clear. Could it be that my school has such old specimens that all the peptidoglycan is long gone and alcohol just washes everything away?

@VanillaSnake21 5 лет назад

I can answer this now that I've finished the semester, by the end I was able to do perfect Gram stains. The reason it could sometimes comes out mixed color or the blue color washes off completely is mostly due to uneven alcohol coverage. You have to place the drop of alcohol all the way at the top of the slide and let it slide down on it's own, also don't follow the directions "until it runs clear" once you see that it's light blue it's fine to stop. It's just a matter of getting the amount and the coverage right. By the way, got an A+ in this class, due in part to videos such as these. Many thanks.

@nalabagam544 5 месяцев назад

What are the other counter stains other than safranin can be used ?

@dr.hannibal8338 2 года назад

Better than my professor explanation

@ayeshaash6571 3 года назад

Thankuu so much it really help me thankss.

@narayanannarayanan317 3 года назад

Thank you for this uploading vedio

@shine9796 5 лет назад

thank you

@zaynmalik879 4 года назад

So awesome thnku so much

@Rahil_Ahmad980 2 года назад

Interesting your videos good amazing

@ruaa9598 4 года назад

Thank u soooo much ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️

@raphaelleteissedre7615 Год назад

I'm here because one of my character is a scientist and I wanted to describe part of the procedure for Gram straining!

@mehmethanyildirim1353 4 года назад

Ooo very difficult but i sure , i will do in this time 😊

@syedibrahim570 4 года назад

Tqs good information sir

@leekspinner 8 месяцев назад

i'm just a random person who thought that gram-negative means something about the weight thank you for education!

@destinybasedoll 4 года назад

how long does it take to perform and analyze a gram stain ?

@heyitsrayrui 2 года назад

Like 15-20 mins?

@khgnew763 2 года назад

at very fast if you add drop of water to bacterial colony, the cells may swell and lyse due to osmotic gradient. better use a drop of isotonic normal saline