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Histological staining: hematoxylin & eosin 

BioVitrum
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The most popular and one of the principal stains in histology is hematoxylin and eosin stain. It gives us an overview of the tissue and its structure. Hematoxylin binds with basophilic structures - for example DNA and RNA. So we can observe nuclei stained in blue or purple color. Eosin binds to acidophilic substances such as positively charged amino acid side chains. So as the result cytoplasm is pink or orange. All samples in laboratory are stained with H&E. There are several different types of hematoxylins and eosins used in histology which will give us different results.
In this video you will see, how we stain slides with different types of hematoxylins and eosins. Finally, we will compare the results.
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3 окт 2024

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Комментарии : 35   
@GonzalesDalidien
@GonzalesDalidien 5 месяцев назад
Hello, thank you for the video Can you please show us how to filter the stain?? We used to get ( impurities, dirty particles, bacterial or fungal like elements) in our hematoxylin stain after a while of using , can you please show the solution and the method of filtration ?
@brkhra.k.a.legatus3183
@brkhra.k.a.legatus3183 5 лет назад
It is a very help video. Thank you so much for your efforts.
@يارافخري
@يارافخري 5 месяцев назад
great! Thank you very much
@mohamedroshdy882
@mohamedroshdy882 3 года назад
Thanks you very much for your show
@unsaltedegg
@unsaltedegg Год назад
Thank you! This helps me a lot!
@Valentina-he2uu
@Valentina-he2uu 4 года назад
Bella zi, mi hai salvato l’esame di istologia 💪🏻
@brennanconner171
@brennanconner171 3 года назад
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@marcelsincere9933
@marcelsincere9933 3 года назад
@Brennan Conner Instablaster :)
@brennanconner171
@brennanconner171 3 года назад
@Marcel Sincere Thanks for your reply. I got to the site through google and I'm in the hacking process now. I see it takes quite some time so I will reply here later when my account password hopefully is recovered.
@brennanconner171
@brennanconner171 3 года назад
@Marcel Sincere It did the trick and I now got access to my account again. I'm so happy! Thanks so much you saved my account !
@marcelsincere9933
@marcelsincere9933 3 года назад
@Brennan Conner Glad I could help =)
@nooriafghan7636
@nooriafghan7636 4 года назад
Please can you tell us combination of hematoxilin and acitic acid mean's how much acitic acid can add to 500ml hematoxilin
@kwabenaadu-twum50
@kwabenaadu-twum50 2 года назад
How would you determine the end point of fixation?
@MohsinAli-cn5wr
@MohsinAli-cn5wr 2 года назад
@BioVitrum, What will be the procedure for detecting the microneedle effected area with H and E staining?
@arshaankhan2564
@arshaankhan2564 3 года назад
Explain is good 🙏
@ernestdutch3586
@ernestdutch3586 4 года назад
Helped me a great deal
@abdifatahfarahhamud6426
@abdifatahfarahhamud6426 4 года назад
Good presenter, i preferred how This cute girl presented with Dignity ! 👏👏👏
@mirraju3744
@mirraju3744 2 года назад
Thanks
@goldenheart1667
@goldenheart1667 3 года назад
Thanks a lot
@muhammadmoazzam2662
@muhammadmoazzam2662 Год назад
Hi, how to make our sample stable on the slide?
@begum2079
@begum2079 3 года назад
Does the time we use for hematoxylin and eozin make big changes?
@NicksLocker
@NicksLocker 2 года назад
Yes, it does
@russ9445
@russ9445 3 года назад
THANK YOUUUUQ
@sachchajay5506
@sachchajay5506 3 года назад
Could you please explain how xyelen make the smear transparent ?
@russ9445
@russ9445 3 года назад
because xylene is a fluid with a property that has high index of refraction giving it a transparent look hence the name clearing, hope this helps :)
@jowairiady1931
@jowairiady1931 4 года назад
Why do we need to dehydrate again after staining?
@lajoswinkler
@lajoswinkler 3 года назад
Because it needs to be cleared and embedded into a mountant with similar refractive index as the glass slide and that mountant is nonpolar.
@jowairiady1931
@jowairiady1931 3 года назад
@@lajoswinkler this is quite late, but thank you for answering 😊💛
@militaryenthusiast1929
@militaryenthusiast1929 Год назад
​@@jowairiady1931woow you came back a year later
@jowairiady1931
@jowairiady1931 Год назад
@@militaryenthusiast1929 HAHAHAHAHHAHA ikr 🤣
@devarapallisubbareddy1139
@devarapallisubbareddy1139 3 года назад
Lab technician work super
@tr4or
@tr4or 11 месяцев назад
❤❤
@_abubakraljelly7343
@_abubakraljelly7343 2 года назад
I like you very match 😍😍😍
@fairy-pc6xo
@fairy-pc6xo Год назад
А на русском слабо????
@냥집사-x1o
@냥집사-x1o 2 года назад
Спасибо 👍
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