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How To Clone Mushrooms 

FreshCap Mushrooms
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Cloning mushrooms is easy! Learn the process used to clone wild mushrooms in the lab, which can eventually be used for commercial cultivation.
In this video, I take a cultivated King Oyster Mushroom, remove a piece of tissue from inside the cap, and clone it on agar.
I'll also show you a few wild clones that I made using this very same process.
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21 сен 2018

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Комментарии : 429   
@thombaz
@thombaz 5 лет назад
Banana for scale, good old times.
@101Skywatcher
@101Skywatcher 4 года назад
I understood that reverence
@mintyfresh2620
@mintyfresh2620 4 года назад
Remote control or coke can also works lol
@nopeno2350
@nopeno2350 4 года назад
Lol
@andrewgoodbody2121
@andrewgoodbody2121 4 года назад
One of us! One of us!
@michaelhurlburt4502
@michaelhurlburt4502 Год назад
I always used a AA battery
@microska2656
@microska2656 4 года назад
This guy looks straight into your soul
@Ankh_Hippy
@Ankh_Hippy 4 года назад
Swampert D. I was literally about to post this 😭😪
@johnpilapil9250
@johnpilapil9250 4 года назад
@@Ankh_Hippy dude same here!
@imamoronand9199
@imamoronand9199 4 года назад
Or slightly above it
@skippydeenice
@skippydeenice 4 года назад
it's the mushrooms
@Ankh_Hippy
@Ankh_Hippy 4 года назад
Chip Osgood he’s seeing the souls of millions and it’s working through the lens ...mushrooms are dope
@woody9283
@woody9283 4 года назад
Thanks for showing what contamination looks like. No one ever shows what to watch out for.
@kaorumugen991
@kaorumugen991 2 года назад
Yeah, but it kind of makes sense that they don't. For every contaminant someone shows you, there's a thousand others they can't. A much better way is to simply learn what healthy mushroom mycelium looks like, then assume everything else is a contaminant.
@LauraMartinez-rf3dm
@LauraMartinez-rf3dm 5 лет назад
I'm so happy I found your videos. I'm a science teacher trying to get my students interested in outdoors and gardening. I think they would be fascinated with growing mushrooms. Also I want to learn myself :) I'm going to check out your website. Any suggestions for first time growing? Thank u!
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Awesome! Glad you like them :) My suggestion would be to start with a "kit" to get an idea for the process- then it's down the rabbit hole- happy growing...
@bananabreadyum9663
@bananabreadyum9663 3 года назад
I wish you were my teacher! Greatings from germany
@Chris-dy5fy
@Chris-dy5fy 2 года назад
Invest in doing things right. If u invest in the right substrates, sterilization procedures, flowhoods or glove box, obtaining sterile cultures... It becomes very easy. It's difficult if u cut corners, and mistakes will cost u more down the road then investing in proper procedure.
@TheMldytwkr85
@TheMldytwkr85 2 года назад
I wish I would've had you as a science teacher. My current Biology Professor down plays anything on mushrooms, in fact he gets annoyed whenever I bring up fungi or mycology. I kinda feel most of the science community has a superiority complex when it comes down to the mycology department?🤷‍♂️
@Budnbuf
@Budnbuf Год назад
Excellent. Anthony you are so good at spreading the word. Thank you and good job.
@caseywillie443
@caseywillie443 5 лет назад
About time man you needed to have done this video long ago. I have been wondering how to do this, Thanks!
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Your right, I've been slacking!
@tomfoolery8100
@tomfoolery8100 5 лет назад
​@@FreshCapMushrooms how much higher is contamination rate if i do it in a plastic box that i cut 2 holes in for handling.
@donutkillmehh
@donutkillmehh 3 года назад
Flow discipline that you were mentioning is something that never occurred to me. That's really smart keeping where you're going with your transfer/clone etc as close as you can to the hood.
@emmanuelsebastiao3176
@emmanuelsebastiao3176 5 лет назад
Thanks for Sharing Tony ... You are a genius. And i Love how you've done your operations so carefully. you are a Champion
@Filekv5
@Filekv5 5 лет назад
I love the content you put out. Keep doi g the great job!
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Thanks so much!!
@Cheddarbob32
@Cheddarbob32 Год назад
Couple questions: Is this tryptic soy agar? Incubate at room temp? How long are the plates good for when stored in fridge or freezer?
@mattmatthews9112
@mattmatthews9112 5 лет назад
Thanks heaps. Lots of helpful information and great examples.
@davidmorrisii69
@davidmorrisii69 2 года назад
Nice video brother, i really appreciate it!!
@tarabonee8855
@tarabonee8855 5 лет назад
Great video...would love to see a video explaining cleaning up a culture also. Oh! I just got the tshirt I ordered in, and duuuudde...I love it! Super quality
@tarabonee8855
@tarabonee8855 5 лет назад
I just saw the transfer video, lol. I must have missed it before.
@lukedepina
@lukedepina 5 лет назад
Love your vids! You're the man
@obertosiciliano1243
@obertosiciliano1243 4 года назад
Great vid!! Thank you for being so thorough
@chrysovalantislamprianidis8893
@chrysovalantislamprianidis8893 4 года назад
Hi and thanks for the videos. They are really instructional and easy to understand.Maybe you answered this one already. Where can we store, what temperature and for how long both the empty agar dishes and the ones containing mycelium.
@filax4500
@filax4500 2 года назад
The amount of eye contact in this video is proportionally equal to its awesomeness!
@rushinbushin
@rushinbushin 2 года назад
The precision makes me so happy
@williamkreth
@williamkreth 2 года назад
Thanks for sharing your knowledge dude appreciate it
@steeveedeee
@steeveedeee 5 лет назад
Awesome!! Thanks brother
@DDawg1144
@DDawg1144 6 месяцев назад
You’ve got a great channel, already made grains thanks to your vid, soon will graduate to agar.
@yangchen7982
@yangchen7982 4 года назад
Love your videos man!!
@lorenzovega8716
@lorenzovega8716 4 года назад
Dude such great vids. This guy is great!
@chucky2959
@chucky2959 4 года назад
That oyster looks so yummy ~
@niranjankanamaluru101
@niranjankanamaluru101 5 лет назад
Excellent work...cheers..
@naesism
@naesism 3 года назад
Hello, thank you for the informative video. I tried the king oyster and it worked, but not for portobellos and enokis. Any suggestions for why that might be the case?
@ljamest2112
@ljamest2112 2 года назад
Thanks Tony!
@celtickitc
@celtickitc 5 лет назад
Really enjoyed your video. Thanks
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Thanks for watching!!
@overratedprogrammer
@overratedprogrammer 2 года назад
You should make a video on maintaining/storing cultures, spawns, and mushrooms
@Abundantman777
@Abundantman777 3 года назад
TY Tony. This a big help to me.
@mycoalliance1909
@mycoalliance1909 5 лет назад
Awesome! I shared this with students from my recent class. Very helpful info. Keep it up =)
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Thanks!! I will definitely keep it up 😀
@robcroyle
@robcroyle 5 лет назад
Best mushroom videos on RU-vid! Every mushroom lover and grower should watch your vids! Video suggestion; how to make a spore print/spore seringe and how to properly store them.
@abdullahzayadin4671
@abdullahzayadin4671 5 лет назад
Can you explain what is the next step in the process? And did you find any useful data answering your question?
@robcroyle
@robcroyle 5 лет назад
@@abdullahzayadin4671 I'm not sure I understand what your asking? As far as making spore prints my research has taught me, you take the mushroom cut off the stalk/stem with sterile scissors or a scalpel, making sure to cut it flush so the cap can rest on the paper, then put a drop of water on the top of the cap and place a glass over the whole thing overnight. To make spore syringes you take the spore prints and put them in the desired amount of water and allow the spores to leave the paper and become suspended in the water. Finally sucking the water up into the sterile syringe. As for storing them I'm still not sure, all I know is keep them out of light and high heat
@dg5450
@dg5450 4 года назад
For active mushrooms check out arvin fortyseven
@daraa151
@daraa151 4 года назад
jackson.wilmes Absolutely, this is the best and most useful videos on mushrooms I have ever came across! Thank you and keep the good work!
@dibdej317
@dibdej317 4 года назад
Thank for sharing. Great video
@drubarlow5017
@drubarlow5017 3 года назад
beautiful work mate
@peregrind9048
@peregrind9048 3 года назад
Thanks for sharing, very informative
@ThatBritishHomestead
@ThatBritishHomestead Год назад
if you grow them in deep multch and keep adding to in with they ever stop growing?
@kickasscorm
@kickasscorm 4 года назад
So brilliant video. Likeable guy too. 10/10!!
@kraghu8648
@kraghu8648 5 лет назад
Excellent video Sir, thank you very mush sir for you are giving good information...
@dzdzful
@dzdzful 3 года назад
Thank you so much, Now I have to find out how to grow mushroom using this culture on petri dish...
@chanteuhtlicuauhtlatoa6115
@chanteuhtlicuauhtlatoa6115 3 года назад
Thak you so much for making these videos!! I have some queations; after you put the mushroom pieces on the agar in Petri dishes, how much days are one going to expect them to be ready, and how is it preferable to store the petri deshis, like in a dark fresh place, with no iight coming in? Then, after we have the spores blooming, can we make them into a liquid cultive?
@Al_i8
@Al_i8 Год назад
thanks Tony
@wilsonndibinze3721
@wilsonndibinze3721 5 лет назад
It's Helpful. Thanks
@woodville8873
@woodville8873 5 лет назад
Great video!
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Thanks!
@Liliisntiiriis
@Liliisntiiriis 3 года назад
Is it better than doing an spore syringe? Could you make a video about pros and cons of each? BTW... I started this rabbit hole because of your videos! Thank you!
@psychologicallyspeaking
@psychologicallyspeaking 4 года назад
Oh SNAP! What is that monster mushie! Wow!
@datnguyen-ul9vm
@datnguyen-ul9vm 5 лет назад
I love your videos but it would be nice if you making a video processing of the mushrooms grown
@poojajalindre7023
@poojajalindre7023 4 года назад
Hi, I have a question. How to now weather my button mushroom fungus is growing good or not? Because this is my first time to grow fungus.
@mihailmihaylov4974
@mihailmihaylov4974 4 года назад
Could tell me what would be the best way to preserve mycelium or spores long-term? Keeping grain spawn / liquid spores / agar dishes / print? My hypothesis would be a spore print taken in a sterile environment and kept in dry sealed jar.
@seraj.
@seraj. 4 года назад
How can you store it and how long does it last?, great video btw
@Maolo92
@Maolo92 Год назад
1. How do you store the agar plates? 2. How long can you store an healthy culture?
@liamfbules2999
@liamfbules2999 4 года назад
Could you use high proof ethanol instead of isopropyl alcohol to avoid making the mushroom inedible? Or does isopropyl just work a lot better than ethanol.
@ixcairogues3699
@ixcairogues3699 5 лет назад
i love the banana for scale
@shaimabukhamseen6451
@shaimabukhamseen6451 3 года назад
Hi, thank you so much for sharing, can I do it with button mushroom ?
@RACHELPARKER1103
@RACHELPARKER1103 4 года назад
SO COOL :D, feel like I'm a scientist!!!
@brokejuanjo
@brokejuanjo 4 года назад
Hi
@diegomartinez4451
@diegomartinez4451 9 месяцев назад
Hi, whats the composition of the agar? tried this exact method but i think i got some contamination from the mushrooms themselves, idk if its because my agar is very rich or what. The control is good so no contamination from that side.
@NicolasSanchez-xl4or
@NicolasSanchez-xl4or 3 года назад
Hi ! I have a question: Which agar you used for cloning mushrooms? Thanks for the video
@acykat2069
@acykat2069 5 лет назад
There is a tiny reishi mushroom growing on a hackberry stump in my yard, but the surface of the stump is completely white in a patch--it looks like a block getting ready to fruit. Being January, it's growing very slowly.. is it enough mushroom to cut off for a tissue culture, or do I need to wait? There's maybe a half inch of red showing on the fruit body.
@timjones5160
@timjones5160 5 лет назад
When doing this do you have to cut from the cap your can you cut from the bottom of stem?
@philq01
@philq01 Год назад
Can you reuse the petri dish? Or must you just throw it away?clean it with alcohol and or expose it to uv light?
@chenjiachen5547
@chenjiachen5547 2 года назад
Where do you store the Petri dishes for further culture? An incubator ? After the mycelium fully takes over, how to store them for long-term use?
@SunnieDIY
@SunnieDIY 3 месяца назад
How long do they last and where to store them once it has developed on the new dish
@mahsas9547
@mahsas9547 4 года назад
I'm trying to grow white button mushrooms at home. I washed everything (jar, knife,...) before I used them, but didn't sterilized them completely. So far so good. No sign of contamination in the jar of cooked grains and the mushroom sample, and the baby mushrooms have started to show up. does this mean I've done everything right?
@user-jn7nf6xu8l
@user-jn7nf6xu8l 2 года назад
after complete colonization of the mycelium on the entire surface of the agar, where and at what temperature and how long can the mycelium be stored?
@emeteriogomez3748
@emeteriogomez3748 2 года назад
youre dope man, thanks
@dariuszwesierski1928
@dariuszwesierski1928 2 года назад
Can i clone mycelium from fruit body straight to sterilized grain? Like You on agar plate, take 3 pcs and throw them to jar
@haangloose
@haangloose Год назад
Great video! Where Do i have to store the Agar plates with the tissue inside?
@OmniClimHazard
@OmniClimHazard 4 года назад
Love these vids! Question. How long does an agar culture last/ whats the best way to store it?
@aleksgregorcic3868
@aleksgregorcic3868 3 года назад
Fridge. I am not sure how long can they last tho
@creatodayin4924
@creatodayin4924 3 года назад
Great helpful video but sir how I store the plates and how long I use a culture for Micilium growth? Lots of love from Asia
@lesliewubbel9157
@lesliewubbel9157 4 года назад
how did you make your set up with air filter HEPA?
@bryanadz
@bryanadz 3 года назад
Hi, How long time you can conserve the Liquid culture and the discs and where ? Thanks
@kezza10mail
@kezza10mail 5 лет назад
Hi, I have a few questions about equiptment, and RE USEAGE of materials, the glass agar jars, and the plastic grow bags, are you able to clean them and re use them ? maybe you could do a video about that, letting us know about what we can re use, and what we need to use new again, thanks again, great videos.
@ethanvoissem9346
@ethanvoissem9346 5 лет назад
I can answer this, most stuff in biology is designed to be disposable, the agar dishes if glass you will wash out media and sterilize them in an autoclave before using again. Most petri dishes like this are plastic and disposable. Most plastic stuff is not designed for reuse even certain metal items like scapel blades are disposable. As a general rule of thumb if you can buy the particular item in bulk (agar dishes, scalpel blades, micrscope slides, syringes, empty grow bags, etc) they are designed to be disposable, I'm sure there are exceptions but I can't think of any. Its a lot more ecomical time and money wise to just buy plastic agar dishes then get glass ones and reclean sterilize and make the growth media each time, off course I recommend having glass ware that is reusable that way if you cannot order supplies you can still work, but when you can go disposable.
@pattybb2823
@pattybb2823 3 года назад
Thanks for the video. Are you scraping from the flesh or gills? Does it matter if it’s either?
@tasteslikepennies2549
@tasteslikepennies2549 5 лет назад
Great video! Cloned!
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Thanks!
@tasteslikepennies2549
@tasteslikepennies2549 5 лет назад
@@FreshCapMushrooms I just watched it for a second time and wanted to point out one thing. Your workspace has a flow Hood. A laminar flow hood is built differently. Just to be super anal and Technical about it. But still super amazing video. Totally filled in some blanks for me in-between the reading and the actual practice of taking tissue samples. Thank you very much for taking the time to make this video!
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Your right, technically this is a "flow cabinet" or similar, not a hood. But I have always called it a flow hood, so old habits I guess. Thanks for watching the video and glad you could take something from it!
@sarajohnson6855
@sarajohnson6855 5 лет назад
Would it be possible to clone polypores with this method? I'm nowhere near your skill level but I hope to get There one day. There are wild strains of chicken of the woods in my area and it would be amazing if I could.
@chrismartin5010
@chrismartin5010 3 года назад
How many times is it possible to ”reclone” ? Meaning you manage to get the fruiting bodies an repeat the operation indefinitely?
@benjaminmance882
@benjaminmance882 3 года назад
How did you get the king oyster long and yellow like that? fantastic.
@revolution5547
@revolution5547 9 месяцев назад
Would it be possible to use a still air box instead of laminar flow hood
@tatanleanduck
@tatanleanduck 11 месяцев назад
Is it true that cloning from fruits that aren't fully mature (still growing) will provide you more aggressive mycelium on agar? I read that somewhere, but it doesn't seem to make much sense to me. The aggressiveness of the mycelium should be based on the genetics of the fruit, not its life stage, right?
@nuflix320
@nuflix320 Год назад
Hi. I've never tried growing mushrooms before. I don't have access to most of the scientific stuff in my country such as petri dishes. Is it possible to clone a store bought mushroom just by using stuff like jars, pressure cooker and homemade still air box. I just want to start growing on a small scale to enjoy the process at home. I also saw some guy grow store bought mushroom on coffee grains in a Jar, idk what was the process called exactly but something like that would be great. I think it would also be a great idea to make a video about it for kids and people with same problem I have.
@rustyshackleford6341
@rustyshackleford6341 4 года назад
Good video man. How long does the culture last in the dish?
@bcleeanderson
@bcleeanderson 4 года назад
So straight forward and concise! The best in the field!
@nahceil
@nahceil 4 года назад
How about smaller mushrooms like pan cyan? Can you still get a tissue on it for agar and get mycelium spores?
@braddishner8597
@braddishner8597 Год назад
I was trying to clone one of my gt and just kept getting stringy cuts. Couldnt seem to get a solid chunk from it. Still took to agar tho🤷‍♂️
@SAMI-gc1hx
@SAMI-gc1hx 2 года назад
How do you prepare the agar mixture? Apart from agar what else do you have to put into it?
@and7barton
@and7barton 5 лет назад
Information is very good - Music is not good. I've been growing mushroom for a year or so. I'm going to try the cloning process.
@klean8198
@klean8198 2 года назад
What type of conditions do you leave the agar plates to grow? Like temp, lighting, etc.
@matthewkbikes6845
@matthewkbikes6845 10 месяцев назад
Temp is about 21 - 27 degrees C, not sure what it is in Fahrenheit
@alexsutton9985
@alexsutton9985 3 года назад
Funky beats brother
@MrKoKo-sb4gu
@MrKoKo-sb4gu 4 года назад
Hey where do I get that stainless steel rack? The rack that ur doing the agars on. I cant find it anywhere. Looks like urs is for agars with the flat wires.
@quickchop1098
@quickchop1098 2 года назад
Are you able to do the same Process but instead of an agar dish, can it be straight into a Liquid?
@daraa151
@daraa151 4 года назад
You are my hero now! Can you please show us how to prepare liquid cultures?
@aleksgregorcic3868
@aleksgregorcic3868 3 года назад
You need to get mushroom spores and mix them with sterilized water
@H3dgi3
@H3dgi3 Год назад
i love the music
@ramonlarson6806
@ramonlarson6806 3 года назад
When you do transfers do you just try to cut the good pieces out?
@mirzarashidbaig
@mirzarashidbaig 5 лет назад
Love your videos. Just curious to know that how many days does it take to fully colonise.
@FreshCapMushrooms
@FreshCapMushrooms 5 лет назад
Thanks! Depends on the species, anywhere from 1 -2 weeks is typical.
@joshbailey7986
@joshbailey7986 2 года назад
Would this work with store bought mushrooms that have been cut days prior and kept in refrigeration? I'm currently doing exactly that and it's been about a week and no changes. By the way I'd still eat that mushroom that you put the alcochol on lol
@fredrikschubert2692
@fredrikschubert2692 3 года назад
Such great videos! Could you put a tissue sample straight into liquid media and expect mycelium growth or is that more likely to result in contaminations?
@dylanjones7798
@dylanjones7798 Год назад
Old comment, but if you're still wondering... It's entirely possible, yes.
@mikehunt4797
@mikehunt4797 3 года назад
Wouldnt more pieces per dish also increase the chances for contamination vs 1 piece?
@crackaassedcracka
@crackaassedcracka 3 года назад
How long are these cultures good for? After the plate is populated, can I put them in the freezer to preserve the culture?
@ye11oman
@ye11oman 4 года назад
I like the banana scale
@gitachaulagain6585
@gitachaulagain6585 3 года назад
is replicating mycelium of oyster from agar to agar many times degrade mushroom yeild
@Aethelvlad
@Aethelvlad 2 года назад
how much agar powder/water do you use in a dish this size?
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