How to Make Agar Nutrient Media for Orchid Seeds - Quick and Easy Process

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In today's episode, I'm sharing how to make the agar media for growing orchid seeds. I'll share my process on how to create the nutrient solution for sowing orchid seeds. This is the germination media used in aseptic orchid seed sowing. It's the first step of several to grow orchids from seed. There are many ways to do this and many variations as well...this is what I do and what works for me. I'll also talk about the reasoning behind this procedure and why I do what I do.
I'm using Phytotech P723 Orchid Sowing Media for epiphytic orchids. Terrestrial orchids require a different recipe for agar media. For sterilization, I steamed the solution for 40 minutes. If you were to autoclave it or use a pressure cooker (121C at 15psi) with the same volume (50ml), then it needs about 15-20 minutes.
References:
- Here but Not: • Growing Orchids From S...
- The Orchid Room: • Unboxing and Flasking ...
- Orchidomania: • Video
- Growing Orchids from Seed (book) by Philip Seaton and Margaret Ramsay
- A Guidebook in Orchid Micropropagation: issuu.com/marc...
- Facebook Group: Orchid Flasking / Orchid Seed Sowing and Seed Exchange
Music: "Freedom" by Atch
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Growing Environment
Orange County, Southern California (USA)
Zone 10B: Subtropical/Hot-semi arid climate - hot, dry summers / mild winters with low to moderate rainfall
Average Summer Outdoor Temperatures: 63-83F
Average Winter Outdoor Temperatures: 48-70F
Average Rainfall: 13 inches/year
Indoor Temperatures: 65-85F
Indoor Humidity 40-60%
East/West facing windows
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THINGS I USE:
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- Sphagnum Moss: amzn.to/2FlqZVx
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- MSU Fertilizer for Well Water: amzn.to/2RfKTne
- Nutricote Slow Release Fertilizer: amzn.to/33kwQlI
- Seaweed Supplement: amzn.to/3ikIdR0
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- Miticide for Spider Mites: amzn.to/2FlAIuO
- pH Meter: amzn.to/3dq5XS9
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- Plant Moisture Sensor: amzn.to/370Bb16
CAMERA GEAR:
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- 35mm Lens - Meike 35mm: amzn.to/3ggTbHr
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DISCLAIMER: Links included in this description may be affiliate links. If you purchase a product with the links provided I may receive a small commission. Thank you for supporting Attainable Green so I can continue to make awesome content for you to enjoy! #orchids #orchidseeds #agarmedia

Опубликовано:

30 сен 2024

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Комментарии : 72

@BBQNBLUES 3 года назад

Excellent Tutorial Jess ! You explain everything in Layman's terms... :)

@AttainableGreen 3 года назад

Thanks! I wanted to keep it simple to show that it’s not hard to do. 😁

@tulsiramdagur9580 Год назад

Hello friend I needed blue berry seeds jarminetion midiya, You help me frends

@purplethumb7887 Год назад

Any success/update? I want to try just as a hobbyist as well. Thanks!

@AttainableGreen Год назад

There may be a playlist that has some updates on these flasks. So far, I have about 5 flasks growing well and the rest are still in experimental phases. I'll put up a new update in early 2023. Thanks for stopping by!

@OrchidsFynbos 3 года назад

Thanks for the update 😊🙏 really excited about where this is going. Great explanation and really good tips. Fully agree with you on starting with the basics and getting that right. The commercial guys have it so easy with their laminar flow and autoclaves 😅. I keep on reminding myself that these techniques were around way before any of the fancy equipment.

@AttainableGreen 3 года назад

Haha yea! Fancy equipment definitely helps speed up the task, but having good technique is better still. A lot can be done with simple items, so I don’t worry about having the “right” things. 😁

@germanplantguy3133 Год назад

Hey I know I am kinda late to the party but I really need to know if its possible to just boil/cook the jars/flasks? I dont have a pressure cooker but I have managed to polinate a orchid of mine and really want to continue the work.

@JustcallmeDebbieB Месяц назад

How did everything go??

@remyvelunta715 Год назад

How to contact you... where could i get the phyto tech sowing medium ?

@paulzwartepoorte4149 Год назад

Hello im looking for media whar can buy in Europa?

@bryandompig5183 Год назад

I don't have this ,can I use ager from the supermarket.

@peacefulearth4us 2 года назад

Thank you for taking your time sharing your knowledge. 🙏 Very interesting information!

@AttainableGreen 2 года назад

You’re welcome! Glad you’re enjoying the videos! 😁

@LAWeather Год назад

Amazing! Thank you for the helpful information 😊

@Graemewalatara Год назад

Hi, Jess thank you for your video about How to Make Agar... Please advise me on where I can purchase Germination media P723? Thank you,

@yennguyen2951 9 месяцев назад

Where do you buy Orchid seeds-phytotech p723? Thank you

@dehawklin 5 месяцев назад

Thank you.. Can I use ordinary nutrient agar instead of this agar... Do u think it wld work?

@mariatheresabalfermosobono2993 2 года назад

Hi, may I know if P723 is good for Phalaenopsis? Thank you.

@AttainableGreen 2 года назад

Yup! P723 can be used for phals. 😀

@dalewinters8701 Год назад

Where can you get this media?

@dontknowwhataplantis 2 года назад

Hey Jess! Thanks for making this video. I've been watching some mushroom cultivation videos and I wonder if I can use mason jars with self-healing injection ports to cut my risk of contamination? Let me know what you think! I think I can instantpot-sterilize my agar solution in a self-healing mason jar, then inject the dry seed + peroxide solution via the self-healing port. Do you know which gauge needles would be large enough for orchid seed?

@AttainableGreen 2 года назад

It might work, I haven’t quite considered it. Some orchid seeds are small, about the size of mushroom spores so those might work. For larger seeds like from complex Dendrobiums or other types, they might be too large for a typical syringe. I had trouble getting some seed out with a plastic 2ml syringe with no needle tip…really just depends on the size of the seed

@dickusmaxximun8126 Год назад

The best way to cut on contamination is to use green seedpods, they come sterile from nature. I flame a lancet, divide the seed-pot and use a sterile tool to pour few seeds in the flask. Peroxide, syringes, Clorox... are noise in the system if you have access to a laminar flow hood.

@HappyDayRoots 10 месяцев назад

😂 was thinking the same thing

@annavoeggia816 3 года назад

Very cool and exciting! Looking forward to seeing your updates on this project.

@AttainableGreen 3 года назад

Thanks! I’ll have a video out tomorrow about flasking the seeds 😁

@cesarb6780 Год назад

Where to buy the powder mix?

@FernandaNascimentoOrchids 3 года назад

Very interesting info. I do not intend to do it but I still like to know how it is done and so I appreciate your vídeo 🙏

@AttainableGreen 3 года назад

Yea no problem! I don’t think it’s for everyone but it’s cool to see how it’s done 😁

@krisaaron5771 2 года назад

Excellent information -- I may give starting orchids from seed a try!

@AttainableGreen 2 года назад

Yea! They’re definitely worth trying and there’s lots of different techniques and ways to go about it. If you’re not successful with one method, there’s many more to test out. 😁

@surajsagar6935 11 месяцев назад

Hi sis i am nepal i want orcid farming nepal can u help me patnar bignes

@yennguyen2951 9 месяцев назад

Thank you for your great information.

@spaideman7850 Год назад

Why don’t u just tell us the ‘powdered solution ‘ name?

@yeoldsniper4767 5 месяцев назад

1:37

@senemkozhokmatova467 Год назад

Öncelikle teşekkürler ederim bi süredir araştırma yapıyorum, sizin sayenizde öğreniyorum😊 kırgızistan Kırgız kızı yım türkiye de yaşıyorum İngilizce öğrenmeye çalışıyorum sizden ricam Turkçe alt yazı bazı yayınlarızda yok lütfen sizden ricam Turkçe alt yazı oluşursa sevinirim sevgili saygıyla goruşmek üzeri 👋

@janswift331 2 месяца назад

You lost me a powdered media? what is that?

@Lunala97 2 года назад

Where did you by the agar mix, was it online and what is the link?

@AttainableGreen 2 года назад

I got it from Phytotech Labs in USA: phytotechlab.com/orchid-seed-sowing-medium-p723.html

@sagartamang7176 8 месяцев назад

Mam how to order agar P723

@woojaepark9915 Год назад

How do l get p723media

@alexandratsy 3 года назад

What an amazing video!

@AttainableGreen 3 года назад

Thank you! 😁

@Running_Putter 5 месяцев назад

Where did you buy Phytotech P723 ?

@AttainableGreen 5 месяцев назад

From the Phytotech website.

@johntee4728 2 года назад

after you planted the seeds whereby you incubate the jar? and for long?

@AttainableGreen 2 года назад

After seeding the jars, I leave it alone in a slightly lighted location. It took about 3 weeks for strong germination and it still continues to grow in flask. I am waiting for the first leaves and roots to grow before I think about reflasking into a larger space. I believe the plants stay in flask anywhere from 1-2 years.

@dickusmaxximun8126 Год назад

If you are doing solid media, there is a germination media and a replate media. The germination depends on the species of orchid. Some take a few weeks, some take much longer. The species and the medium you are using makes the difference. I like to move my protocorns to my replated media and let them to growth there. Sometimes you have to replate your replate if all your protocorns made it to orchids.

@pookahmonster 2 года назад

Nicely done 💝

@AttainableGreen 2 года назад

Thanks! 😁

@eddy8828 Год назад

Excellent video. Thanks for the tips. 👍👏

@wandashaw3551 3 года назад

Very interesting! 🐈😍

@AttainableGreen 3 года назад

Thanks! 😁

@leopillay8939 3 года назад

How can I make my own powder medium we materials that are easy accessible

@AttainableGreen 3 года назад

I have not made my own agar powder medium so I cannot vouch for the efficacy of homemade media. There’s recipes in this link that have been tested and used in lab settings: issuu.com/marcoacuna/docs/a_guidebook_in_orchid_micropropagat/30

@leopillay8939 3 года назад

@@AttainableGreen hi do u have a recipe that requires ingredients that is accessible for growing orchid seeds plz

@dickusmaxximun8126 Год назад

You can search peer review papers. There are some specially in Brazil and Peru using krystalon orange fertilizer, sugar and cooking agar and seems to work fine.

@varolturkyilmaz1588 2 года назад

O kullandığınız siyah toz veya sıvı nedir

@AttainableGreen 2 года назад

Kömür ve diğer besin maddeleri içeren agar agardır.

@varolturkyilmaz1588 2 года назад

Diğer besin maddeleri nelerdir

@AttainableGreen 2 года назад

Bu web sitesindeki besin listesi: phytotechlab.com/orchid-seed-sowing-medium-p723.html

@bryandompig5183 2 года назад

The media leaf it out side for 3,day

@AttainableGreen 2 года назад

The media is put away for 3 days to check for contamination. If it does not grow mold or bacteria, it’s good to use. Not for outside.

@YnseSchaap 3 года назад

I sometimes get plants (seedlings) that could need some more tlc would agar be a good idea to grow them on ?

@AttainableGreen 3 года назад

If the plants are small enough, it may benefit from a little more time in agar. It would need more nutrients than the P723 media since that is usually used to germinate seeds. P748 or something similar (replate or maintenance media) would be better since it has more nutrients to sustain a larger plant.

@YnseSchaap 3 года назад

@@AttainableGreen I will give it a go. Searching for the media I found a website that shows step by step what you did in your video including building a flowhood to keep it all sterile really interesting and how to propagate from nodes as well 😁

@AttainableGreen 3 года назад

Super cool! 😁

@matwhite6481 3 года назад

I couldnt follow the end because her voice is extremely irritating!

@anornpath1587 2 года назад

Aww boohoo

@redbutterfly88 Год назад

I had tried this before but i fail. But its my fault not sterile properly it gets moldy since then i didnt try again. This instant version maybe ill consider.