How To Make Stock Solutions For Plant Growth Regulators 

Thanks!

Thanks, will do!

Powder form hormone can be used until the expiry date of the product (check the bottle to look for the expiry date), provided the opened bottle is stored in a suitable environment.

Yes, I'll add it to my list!

Thanks for your feedback!!

You really don’t want to grow cannabis with these hormones unless you really know what you’re doing. They’re great for houseplants, not so much for ingesting…

Glad to know that our video was useful to you!

1/2 MS media with 1.0 ml/l BA, 0.1 ml/l NAA, 1 ml/l PPM, 25 g of sucrose, and 3 g of gallan gum or 8 g of agar. I'll add aquatic mosses to my list.

Mosses are very diffucult. İ Tried 3 4 another ways and no result. Always dead or contamined

tissue culture is a multistep process. contamination can occur because of several reasons, including using unsterilized water, culture vessels, equipment, and tools, improper explant sterilization, stuffed culture room, through the air when the process is not performed under complete sterilized and closed conditions, etc.

Sure. We'll update you if get our hands on Homalomena lants.

Millipore filters that attach to a syringe work fantastic. That's their brand name and they come pre sterilised. just rip open and used in hood.

Great questions Danii! 1. of course you can sterilize in the microwave. However, you must know the working parameters of your microwave and the knowledge of a suitable temperature and time for sterilization. Also, be on the lookout for how much media you add to each vessel. we have a video on our channel for media sterilization using microwaves. You may find it useful. 2. syringe filter is suitable for plant hormones that heat labile and can not be sterilized. There are easy to use. You have to use a suitable filter with an appropriate filter size for the hormones. directly place them inside the needle, take the hormone and add it to sterilized media. Everything should be performed strictly under sterile conditions, mainly laminar flow hood. Unfortunately currently, we don't have any videos on it. But, we will try to make them in near future soon. Thanks!

Dimethyl Sulfoxide 60 mg/ml

@@PlantCellTechnology Muchas gracias !! 😊

Powder can be stored at room temperature and the stock solutions in the fridge.

I'll add it to my list!

The vitamins included in the traditional MS media can be autoclaved. Some more specialized media are completely made out of scratch, where some of the vitamins are added after autoclave. Some labs also like to add the vitamins, PGRs, and antibiotics after sterilizing the media in the autoclave. Some of those they have to, and the others ones do it because it is their protocol. They use a syringe filter.

@@PlantCellTechnology Thank you!

@@PlantCellTechnology What is the size of syringe filter ? 0.25microns ? I understand that Quercetin filtered through 0,45 microns become just half from unfiltered Quercetin. I do not know about other substances.

@@funny11744 yeah generally 0.22 microns is favored for plant hormones. I didn't get what about quercetin you are asking for. Can you reframe your question?

@@PlantCellTechnology I want to mean that after filtration through 0,22 microns It is a big chance that the hormone or any substance to remain lower quantity : the filter itself could retain part of that substance ! I read about Quercetin filtered through 0,45 microns - will remain only about 50% from quercetin .

You weigh the activated charcoal in the required concentration ( gram or milligram per liter, as per your need) and add it to the media. It's similar to the addition of other elements in the media.

@@PlantCellTechnology Thank You sir. Do you have any tips on how to sterilize a very small seeds of plant?

The concentration depends on what plant you are working on. It's always best to follow a specific protocol for each plant.

I recommend having a dedicated fridge or at least keeping them in a box with a lock so children can't access them. We use our own (Plant Cell Technology's) hormones. They are very effective in tissue culture. you can get them here: plantcelltechnology.com/collections/plant-growth-regulators

You can sterilize synthetic PGRs with the media. However, filter sterilization is generally required for natural PGRs. A stock solution of hormones is used to directly take the amount of hormone required while preparing tissue culture media. It saves time.

Sure! Meanwhile, you can check our blog on the same!

The PGRs are sterilized with the media in the autoclave/pressure cooker. If they are heat sensitive, with a syringe and a filter after the media has cooled down.

If I can get some seeds.

@@PlantCellTechnology You need seeds to clone a tree? I thought you would need a leaf or stem to clone a tree.

@@PlantCellTechnology Getting seed would not work because then you would be cloning a seedling instead of the mother tree. This would result in loosing the characteristics that I would be trying to replicate. If a seedling was cloned then the protocol may be different for the original mother tree correct?

@@MrWhiltetail Try FSAC: fragmented shoot apex culture. I did this in 1989 on grape vines.

Google search by name. Some companies send documents in which the right way to use the chemical is mentioned.

you need PGRs for tissue culture. They are required for plants' growth.

@@PlantCellTechnology where can I buy PGRs?

@@nikkilynick9606 You can google the websites that sell PGRs. There are many.

You can get it from our store: plantcelltechnology.com/products/indole-3-butyric-acid-iba-solution-1mg-1ml

Here are some videos that you may find useful: www.youtube.com/@PlantCellTechnology/search?query=monstera